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6.1.1.6: lysine-tRNA ligase

This is an abbreviated version!
For detailed information about lysine-tRNA ligase, go to the full flat file.

Word Map on EC 6.1.1.6

Reaction

ATP
+
L-lysine
+
tRNALys
=
AMP
+
diphosphate
+
L-lysyl-tRNALys

Synonyms

Ap4A synthase, BBA_03037, class 1 lysyl tRNA synthetase, class I LysRS, class I lysyl-tRNA synthetase, class II lysyl-tRNA synthetase, cyto-LysRS, cytoKARS, cytoplasmic lysyl-tRNA synthetase, FOSTERSO_4045, GsLysRS, hKRS, KARS, KRS, KRS-1, L-Lysine-transfer RNA ligase, lysine aminoacyl-tRNA synthetase, Lysine translase, Lysine--tRNA ligase, Lysine-tRNA synthetase, LysRS, LysRS-I, LysRS-II, LysRS1, LysRS2, lysS, lysS2, LysU, LysX, lysyl tRNA synthetase, Lysyl-transfer ribonucleate synthetase, Lysyl-transfer RNA synthetase, Lysyl-tRNA synthetase, lysylphosphatidylglycerol biosynthesis bifunctional protein, mito-LysRS, mitochondrial lysyl-tRNA synthetase, mitoKARS, More, Msk1p, MSMEG_3796, MSMEG_6094, MSMEI_3707, MXAN_4731, PF3D7_1350100, PfKRS, preMsk1p, Synthetase, lysyl-transfer ribonucleate, tRK1

ECTree

     6 Ligases
         6.1 Forming carbon-oxygen bonds
             6.1.1 Ligases forming aminoacyl-tRNA and related compounds
                6.1.1.6 lysine-tRNA ligase

Molecular Weight

Molecular Weight on EC 6.1.1.6 - lysine-tRNA ligase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100000
-
sucrose density gradient centrifugation
109000
-
gel filtration
112000
-
equilibrium sedimentation measurement
114000
-
small-angle X-ray scattering
120000
129000
-
gel filtration
138000
-
equilibrium sedimentation measurement
150000
240000
24700
-
recombinant N-terminal extension, gel filtration
270000
calculated mass, alpha2:alpha2 tetramer. This tetramer is probably functional for aminoacylation, because docking of tRNA molecules to the tetrameric LysRS seems not to cause steric clash
57700
58000
62000
-
2 * 62000, N-terminally truncated recombinant enzyme, SDS-PAGE
62000 - 63000
-
isozyme packaged into virions, proteolytically truncated, gel filtration
64000
predominantly as the 64 kDa processed form in the mitochondrial fraction, Western blot
65000
2 * 65000, SDS-PAGE
66000
-
2 * 66000, SDS-PAGE
68000
recombinant His-tagged enzyme, gel filtration
69000
unprocessed form (i.e. still containing the N-terminal mitochondrial targeting signal) in the cytosolic fraction, Western blot
69700
-
SDS-PAGE
70000
-
cytoplasmic isozyme, gel filtration
70600
recombinant enzyme, gel filtration
72000
-
1 * 72000 + 1 + 62000, SDS-PAGE
79000
-
2 * 79000, SDS-PAGE
82000
wild-type CAT and CAT mutants, SDS-PAGE
8608
-
recombinant N-terminal extension, mass spectrometry
8741
-
recombinant N-terminal extension, DNA sequence determination
90000
-
gel filtration
9104
-
recombinant N-terminal extension, centrifugation sedimentation equilibrium