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6.1.1.6: lysine-tRNA ligase

This is an abbreviated version!
For detailed information about lysine-tRNA ligase, go to the full flat file.

Word Map on EC 6.1.1.6

Reaction

ATP
+
L-lysine
 +
tRNALys
=
AMP
 +
diphosphate
 +
L-lysyl-tRNALys

Synonyms

Ap4A synthase, BBA_03037, class 1 lysyl tRNA synthetase, class I LysRS, class I lysyl-tRNA synthetase, class II lysyl-tRNA synthetase, cyto-LysRS, cytoKARS, cytoplasmic lysyl-tRNA synthetase, FOSTERSO_4045, GsLysRS, hKRS, KARS, KRS, KRS-1, L-Lysine-transfer RNA ligase, lysine aminoacyl-tRNA synthetase, Lysine translase, Lysine--tRNA ligase, Lysine-tRNA synthetase, LysRS, LysRS-I, LysRS-II, LysRS1, LysRS2, lysS, lysS2, LysU, LysX, lysyl tRNA synthetase, Lysyl-transfer ribonucleate synthetase, Lysyl-transfer RNA synthetase, Lysyl-tRNA synthetase, lysylphosphatidylglycerol biosynthesis bifunctional protein, mito-LysRS, mitochondrial lysyl-tRNA synthetase, mitoKARS, More, Msk1p, MSMEG_3796, MSMEG_6094, MSMEI_3707, MXAN_4731, PF3D7_1350100, PfKRS, preMsk1p, Synthetase, lysyl-transfer ribonucleate, tRK1

ECTree

     6 Ligases
         6.1 Forming carbon-oxygen bonds
             6.1.1 Ligases forming aminoacyl-tRNA and related compounds
                6.1.1.6 lysine-tRNA ligase

Cloned

Cloned on EC 6.1.1.6 - lysine-tRNA ligase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a Bacillus subtilis strain NF54 expresses the Bacillus cereus class I LysK under the control of its natural promoter and T box regulatory element in single copy at the amyE locus and the endogenous lysS gene is partially deleted, the transfomred strain is viable
-
COS7 cells are cotransfected with a plasmid containing HIV-1 proviral DNA and a plasmid containing cDNA coding for either full-length human LysRS or a truncated LysRS variant in which the N-terminal 65 amino acids have been deleted (DELTA1-65 LysRS). N-terminally truncated LysRS, which binds poorly to tRNALys, does not increase tRNALys packaging into viruses, while C-terminally truncated LysRS (DELTA452-597), which binds to but does not aminoacylate tRNALys, still facilitates an increase in tRNALys packaging into virions
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DNA and amino acid sequence determination and analysis, expression as T7-tagged enzyme in Escherichia coli BL21(DE3)
DNA sequence determination
DNA sequence determination and analysis, genomic organization, expression of cytoplasmic isozyme in Escherichia coli strain BL21(DE3) as His-tagged enzymes, expression in human osteosarcoma cell line 143B
DNA sequence determination and analysis, genomic organization, expression of mitochondrial isozyme in Escherichia coli strain BL21(DE3) as His-tagged enzymes, expression in human osteosarcoma cell line 143B
expressed in COS-7 cells, expressed in hSOD1 transgenic mice
expressed in Escherichia coli
expressed in Escherichia coli and in Zea mays
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3+ RPC) cells
-
expressed in Escherichia coli Tuner (DE3) cells
-
expressed in Escherichia coli XL1-Blue cells
-
expressed in Novablue Escherichia coli cells
expressed in the bacterial strain BL21 (DE3) CodonPlus using the vectors pET20b, both DELTAS70-T584 and full length LysRS
expressed in TOP10 cells
expressed in yeast
-
expression in Escherichia coli
-
expression in strain BL21(DE3)
-
expression in strain BL21(DE3) as His-tagged protein
-
expression of His-tagged LysRS in Escherichia coli
expression of the C-terminally His-tagged N-terminal extension of the enzyme in Escherichia coli strain BL21(DE3)
-
gene KARS, DNA and amino acid sequence determination and analysis, genotyping
gene KARS, recombinant expression of Strep-tagged enzyme in 293-F cells
-
gene krs or BBA_03037, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis
gene lysS, constitutive expression in Escherichia coli DH5alpha, functional complementation of a class II-enzyme-deficient Escherichia coli mutant only with addition of exogenous lysine
-
gene lysS, constitutive expression in Escherichia coli DH5alpha, functional complementation of a class II-enzyme-deficient Escherichia coli mutant up to 40°C growth temperature with or without addition of exogenous lysine
-
gene lysS, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in conditional expression strains of Mycobacterium smegmatis strain mc2155
gene lysS, recombinant expression of His-tagged enzyme in Escherichia coli
gene lysS, sequence comparisons, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain C41(DE3)
gene lysS2, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in conditional expression strains of Mycobacterium smegmatis
overexpression in Escherichia coli
-
overexpression in strain JM101
-
overexpresssion of His-tagged enzyme in Escherichia coli
-
recombinant expression
recombinant expression of enzyme LysRS in Escherichia coli strain BL21(DE3) that also contains pEVOL-p-benzoyl-L-phenylalanine (Bpa) expressing the orthogonal suppression system, namely Methanococcus jannaschii tRNATyr,CUA, an amber suppressor tRNA derived from Methanococcus jannaschii tRNATyr, and Methanococcus jannaschii TyrRSBpa a mutant of Methanococcus jannaschii tyrosyl-tRNA synthetase that specifically aminoacylates Methanococcus jannaschii tRNATyr,CUA with Bpa
transfection of RBL-2H3 cell
-
wild-type and mutant enzymes, expression in Escherichia coli BL21(DE3)
-