6.1.1.5: isoleucine-tRNA ligase
This is an abbreviated version!
For detailed information about isoleucine-tRNA ligase, go to the full flat file.
Word Map on EC 6.1.1.5
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6.1.1.5
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synthetases
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aminoacyl-trna
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aminoacylation
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isoleucylation
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valyl-trna
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misactivated
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methionyl-trna
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leurs
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mischarged
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pseudomonic
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post-transfer
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noncognate
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valrs
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mupirocin-resistant
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misacylated
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anticodons
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aarss
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kmsks
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glnrs
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trna-dependent
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pretransfer
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lysyl-trna
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molecular biology
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medicine
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drug development
- 6.1.1.5
- synthetases
- aminoacyl-trna
- aminoacylation
-
isoleucylation
- valyl-trna
-
misactivated
- methionyl-trna
- leurs
-
mischarged
-
pseudomonic
-
post-transfer
-
noncognate
- valrs
-
mupirocin-resistant
-
misacylated
-
anticodons
-
aarss
-
kmsks
- glnrs
-
trna-dependent
-
pretransfer
- lysyl-trna
- molecular biology
- medicine
- drug development
Reaction
Synonyms
EcIleRS, IARS2, Ile-tRNA synthetase, IleRS, ileS, ileS1, ileS2, IRS, Isoleucine translase, Isoleucine--tRNA ligase, Isoleucine-transfer RNA ligase, Isoleucine-tRNA synthetase, isoleucyl tRNA synthetase, Isoleucyl-transfer ribonucleate synthetase, Isoleucyl-transfer RNA synthetase, Isoleucyl-tRNA synthetase, mitochondrial isoleucyl-tRNA synthetase, More, mt isoleucyl-tRNA synthetase, mt-IleRS, Mupirocin resistance protein, ScIleRS, SgIleRS, Synthetase, isoleucyl-transfer ribonucleate
ECTree
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Reference
Reference on EC 6.1.1.5 - isoleucine-tRNA ligase
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Kohda, D.; Yokoyama, S; Miyazawa, T.
Thermostable valyl-tRNA, isoleucyl-tRNA and methionyl-tRNA synthetases from an extreme thermophile Thermus thermophilus HB8. Protein structure and Zn2+ binding
FEBS Lett.
174
20-23
1984
Thermus thermophilus, Thermus thermophilus HB8 / ATCC 27634 / DSM 579
Goldgur, Y.; Safro, M.
Aminoacyl-tRNA synthetases from Haloarcula marismortui: an evidence for a multienzyme complex in a procaryotic system
Biochem. Mol. Biol. Int.
32
1075-1083
1994
Haloarcula marismortui
Norcum, M.T.
Isolation and electron microscopic characterization of the high molecular mass aminoacyl-tRNA synthetase complex from murine erythroleukemia cells
J. Biol. Chem.
264
15043-15051
1989
Mus musculus
Yanagisawa, T.; Lee, J.T.; Wu, H.C.; Kawakami, M.
Relationship of protein structure of isoleucyl-tRNA synthetase with pseudomonic acid resistance of Escherichia coli
J. Biol. Chem.
269
24304-24309
1994
Escherichia coli
Glasfeld, E.; Landro, J.A.; Schimmel, P.
C-terminal zinc-containing peptide required for RNA recognition by a class I tRNA synthetase
Biochemistry
35
4139-4145
1996
Escherichia coli
Jakubowski, H.
Proofreading in trans by an aminoacyl-tRNA synthetase. A model for single site editing by isoleucyl-tRNA synthetase
Nucleic Acids Res.
24
2505-2510
1996
Escherichia coli
Zhou, L.; Rosevear, P.R.
Mutation of the carboxy terminal zinc finger of E. coli isoleucyl-tRNA synthetase alters zinc binding and aminoacylation activity
Biochem. Biophys. Res. Commun.
216
648-654
1995
Escherichia coli
Auld, D.S.; Schimmel., P.
Switching recognition of two tRNA synthetases with an amino acid swap in a designed peptide
Science
267
1994-1996
1995
Escherichia coli
Landro, J.A.; Schmidt, E.; Schimmel, P.
Thiol ligation of two zinc atoms to a class I tRNA synthetase. Evidence for unshared thiols and role in amino acid binding and utilization
Biochemistry
33
14213-14220
1994
Escherichia coli
Xu, B.; Trawick, B.; Krudy, G.A.; Phillips, R.M.; Zhou.L.; Rosevear, P.R.
Probing the metal binding sites of E. coli isoleucyl-tRNA synthetase
Biochemistry
33
398-402
1994
Escherichia coli
Airas, R.K.
Analysis of the isoleucyl-tRNA synthetase reaction by total rate equation. Magnesium and spermidine in the tRNA kinetics
Eur. J. Biochem.
210
451-454
1992
Escherichia coli, Escherichia coli B / ATCC 11303
Schimmel, P.; Landro, J.A.; Schidt, E.
Evidence for distinct locations for metal binding sites in two closely related class I tRNA synthetases
J. Biomol. Struct. Dyn.
11
571-581
1993
Escherichia coli
Glasfeld, E.; Schimmel, P.
Zinc-dependent tRNA binding by a peptide element within a tRNA synthetase
Biochemistry
36
6739-6744
1997
Escherichia coli
Racher, K.I.; Kalmar, G.B.; Borgford, T.J.
Expression and characterization of a recombinant yeast isoleucyl-tRNA synthetase
J. Biol. Chem.
266
17158-17164
1991
Saccharomyces cerevisiae
Englisch, U.; Englisch, S.; Markmeyer, P.; Schischkoff, J.; Sterbach, H.; Kratzin, H.; Cramer, F.
Structure of the yeast isoleucyl-tRNA synthetase gene (ILS1). DNA-sequence, amino-acid sequence of the proteolytic peptides of the enzyme and comparison of the structure to those of other known aminoacyl-tRNA synthetases
Biol. Chem. Hoppe-Seyler
368
971-979
1987
Saccharomyces cerevisiae
Jenal.U.; Rechsteiner, T.; Tan, P.Y.; Buhlmann, E.; Meile, L.; Leisinger, T.
Isoleucyl-tRNA synthetase of Methanobacterium thermoautotrophicum Marburg. Cloning of the gene, nucleotide sequence, and localization of a base change conferring resistance to pseudomonic acid
J. Biol. Chem.
266
10570-10577
1991
Methanothermobacter thermautotrophicus, Methanothermobacter thermautotrophicus Marburg / DSM 2133
Charlier, J.; Grosjean, H.
Isoleucyl-transfer ribonucleic acid synthetase from Bacillus stearothermophilus. Properties of the enzyme
Eur. J. Biochem.
25
163-174
1972
Geobacillus stearothermophilus
Grosjean, H.; Charlier, J.
Comparative studies on the isoleucyl-tRNA synthetases from Bacillus stearothermophilus and Escherichia coli. II. The effect of magnesium ions in the transacylation reaction
FEBS Lett.
18
342-346
1971
Geobacillus stearothermophilus, Escherichia coli
Steinmetz Kayne , M.; Cohn, M.
Cation requirements of isoleucyl-tRNA synthetase from Escherichia coli
Biochem. Biophys. Res. Commun.
46
1285-1291
1972
Escherichia coli
Holler, E.
Isoleucyl transfer ribonucleic acid synthetase of Escherichia coli B. Effects of magnesium and spermine on the amino acid activation reaction
Biochemistry
12
1142-1149
1973
Escherichia coli, Escherichia coli B / ATCC 11303
Durekovic, A.; Flossdorf, J.; Kula, M.R.
Isolation and properties of isoleucyl-tRNA synthetase from Escherichia coli MRE 600
Eur. J. Biochem.
36
528-533
1973
Escherichia coli, Escherichia coli MRE 600
Carr, A.C.; Igloi, G.L.; Penzer, G.R.; Plumbridge, J.A.
The effect of spermine anf Mg2+ on the catalytic mechanism of isoleucine:tRNA ligase
Eur. J. Biochem.
54
169-173
1975
Escherichia coli, Escherichia coli B / ATCC 11303
Wakagi, T.; Ohta, T.; Imahori, K.
Studies on isoleucyl-tRNA synthetase of an extreme thermophile, Thermus thermophilus HB8
Agric. Biol. Chem.
39
1573-1580
1975
Thermus thermophilus, Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
Hammer-Raber, B.; Rainey, P.M.; Holler, E.
L-Isoleucyl-tRNA synthetase aus E. coli. pH-Abhängigkeit der Enzym-Substrat Komplexbildung
Hoppe-Seyler's Z. Physiol. Chem.
357
295
1976
Escherichia coli
-
Moe, J.G.; Piszkiewicz, D.
Purification of isoleucyl transfer ribonucleic acid synthetase by affinity chromatography on blue dextran-Sepharose
FEBS Lett.
72(1)
147-150
1976
Escherichia coli, Escherichia coli B / ATCC 11303
Freist, W.; von der Haar, F.; Faulhammer, H.; Cramer, F.
Valyl-tRNA, isoleucyl-tRNA and tyrosyl-tRNA synthetase from baker's yeast. Substrate specificity with regard to ATP analogs and mechanism of the aminoacylation reaction
Eur. J. Biochem.
66
493-497
1976
Saccharomyces cerevisiae
Igarashi, K.; Eguchi, K.; Tanaka, M.; Hirose, S.
Effect of polyamines on isoleucyl-tRNA formation by rat liver isoleucyl-tRNA synthetase
Eur. J. Biochem.
82
301-307
1978
Rattus norvegicus
Hughes, J.; Mellows, G.
Inhibition of isoleucyl-transfer ribonucleic acid synthetase in Escherichia coli by pseudomonic acid
Biochem. J.
176
305-318
1978
Escherichia coli
Freist, W.; von der Haar, F.; Cramer, F.
Isoleucyl-tRNA synthetase from baker's yeast
Eur. J. Biochem.
119
151-164
1981
Saccharomyces cerevisiae
Lazard, M.; Mirande, M.; Waller, J.P.
Purification and characterization of the isoleucyl-tRNA synthetase component from the high molecular weight complex of sheep liver. A hydrophobic metalloprotein
Biochemistry
24
5099-5106
1985
Ovis aries
Kiener, A.; Rechsteiner, T.; Leisinger, T.
Mutation to pseudomonic acid resistance of Methanobacterium thermoautotrophicum leads to an altered isoleucyl-tRNA synthetase
FEMS Microbiol. Lett.
33
15-18
1986
Methanothermobacter thermautotrophicus, Methanothermobacter thermautotrophicus Marburg / DSM 2133
-
Freist, W.; Sternbach, H.; Cramer, F.
Isoleucyl-tRNA synthetase from baker's yeast and from Escherichia coli MRE 600. Discrimination of 20 amino acids in aminoacylation of tRNAIle-C-C-A(3'NH2)
Eur. J. Biochem.
169
33-39
1987
Saccharomyces cerevisiae, Escherichia coli, Escherichia coli MRE 600
Crasto, C.F.; Forrest, A.K.; Karoli, T.; March, D.R.; Mensah, L.; O'Hanlon, P.J.; Nairn, M.R.; Oldham, M.D.; Yue, W.; Banwell, M.G.; Easton, C.J.
Synthesis and activity of analogues of the isoleucyl tRNA synthetase inhibitor SB-203207
Bioorg. Med. Chem.
11
2687-2694
2003
Staphylococcus aureus, Rattus norvegicus, Staphylococcus aureus WCUH29
Lee, J.; Kang, S.U.; Kim, S.Y.; Kim, S.E.; Kang, M.K.; Jo, Y.J.; Kim, S.
Ester and hydroxamate analogues of methionyl and isoleucyl adenylates as inhibitors of methionyl-tRNA and isoleucyl-tRNA synthetases
Bioorg. Med. Chem. Lett.
11
961-964
2001
Escherichia coli
Lee, J.; Kang, S.U.; Kim, S.Y.; Kim, S.E.; Job, Y.J.; Kim, S.
Vanilloid and isovanilloid analogues as inhibitors of methionyl-tRNA and isoleucyl-tRNA synthetases
Bioorg. Med. Chem. Lett.
11
965-968
2001
Escherichia coli
Lee, J.; Kim, S.E.; Lee, J.Y.; Kim, S.Y.; Kang, S.U.; Seo, S.H.; Chun, M.W.; Kang, T.; Choi, S.Y.; Kim, H.O.
N-Alkoxysulfamide, N-hydroxysulfamide, and sulfamate analogues of methionyl and isoleucyl adenylates as inhibitors of methionyl-tRNA and isoleucyl-tRNA synthetases
Bioorg. Med. Chem. Lett.
13
1087-1092
2003
Escherichia coli
Pope, A.J.; Lapointe, J.; Mensah, L.; Benson, N.; Brown, M.J.; Moore, K.J.
Characterization of isoleucyl-tRNA synthetase from Staphylococcus aureus. I: Kinetic mechanism of the substrate activation reaction studied by transient and steady-state techniques
J. Biol. Chem.
273
31680-31690
1998
Staphylococcus aureus
Pope, A.J.; Moore, K.J.; McVey, M.; Mensah, L.; Benson, N.; Osbourne, N.; Broom, N.; Brown, M.J.; O'Hanlon, P.
Characterization of isoleucyl-tRNA synthetase from Staphylococcus aureus. II. Mechanism of inhibition by reaction intermediate and pseudomonic acid analogues studied using transient and steady-state kinetics
J. Biol. Chem.
273
31691-31701
1998
Staphylococcus aureus
Pope, A.J.; McVey, M.; Fantom, K.; Moore, K.J.
Effects of substrate and inhibitor binding on proteolysis of isoleucyl-tRNA synthetase from Staphylococcus aureus
J. Biol. Chem.
273
31702-31706
1998
Staphylococcus aureus
Nathanson, L.; Deutscher, M.P.
Active aminoacyl-tRNA synthetases are present in nuclei as a high molecular weight multienzyme complex
J. Biol. Chem.
275
31559-31562
2000
Cricetulus griseus, Oryctolagus cuniculus
Nakama, T.; Nureki, O.; Yokoyama, S.
Structural basis for the recognition of isoleucyl-adenylate and an antibiotic, mupirocin, by isoleucyl-tRNA synthetase
J. Biol. Chem.
276
47387-47393
2001
Thermus thermophilus, Staphylococcus aureus (P41368), Staphylococcus aureus, Thermus thermophilus HB8 / ATCC 27634 / DSM 579
Yanagisawa, T.; Kawakami, M.
How does Pseudomonas fluorescens avoid suicide from its antibiotic pseudomonic acid?: Evidence for two evolutionarily distinct isoleucyl-tRNA synthetases conferring self-defense
J. Biol. Chem.
278
25887-25894
2003
Pseudomonas fluorescens, Pseudomonas fluorescens (Q8L1B1), Pseudomonas fluorescens NCIB 10586, Pseudomonas fluorescens NCIB 10586 (Q8L1B1)
Fukunaga, R.; Fukai, S.; Ishitani, R.; Nureki, O.; Yokoyama, S.
Crystal structures of the CP1 domain from Thermus thermophilus isoleucyl-tRNA synthetase and its complex with L-valine
J. Biol. Chem.
279
8396-8402
2004
Thermus thermophilus (P56690), Thermus thermophilus
Silvian, L.F.; Wang, J.; Steitz, T.A.
Insights into editing from an Ile-tRNA synthetase structure with tRNAIle and mupirocin
Science
285
1074-1077
1999
Staphylococcus aureus (P41972), Staphylococcus aureus
Kim, S.E.; Kim, S.Y.; KI, S.; Kang, T.; Lee, J.
Deoxyribosyl analogues of methionyl and isoleucyl sulfamate adenylates as inhibitors of methionyl-tRNA and isoleucyl-tRNA synthetase
Bioorg. Med. Chem. Lett.
15
3389-3393
2005
Escherichia coli
Zhai, Y.; Nawaz, M.H.; Lee, K.W.; Kirkbride, E.; Briggs, J.M.; Martinis, S.A.
Modulation of substrate specificity within the amino acid editing site of leucyl-tRNA synthetase
Biochemistry
46
3331-3337
2007
Escherichia coli
Yang, J.A.; Park, D.W.; Sohn, J.W.; Yang, I.S.; Kim, K.H.; Kim, M.J.
Molecular analysis of isoleucyl-tRNA synthetase mutations in clinical isolates of methicillin-resistant Staphylococcus aureus with low-level mupirocin resistance
J. Korean Med. Sci.
21
827-832
2006
Staphylococcus aureus (P41972), Staphylococcus aureus
Fukunaga, R.; Yokoyama, S.
Structural basis for substrate recognition by the editing domain of isoleucyl-tRNA synthetase
J. Mol. Biol.
359
901-912
2006
Thermus thermophilus (P56690), Thermus thermophilus
Shimizu, T.; Usui, T.; Fujikura, M.; Kawatani, M.; Satoh, T.; Machida, K.; Kanoh, N.; Woo, J.T.; Osada, H.; Sodeoka, M.
Synthesis and biological activities of reveromycin A and spirofungin A derivatives
Bioorg. Med. Chem. Lett.
18
3756-3760
2008
Homo sapiens
De Pascale, G.; Lloyd, A.J.; Schouten, J.A.; Gilbey, A.M.; Roper, D.I.; Dowson, C.G.; Bugg, T.D.
Kinetic characterisation of lipid II-Ala:alanyl tRNA ligase (MurN) from Streptococcus pneumoniae using semi-synthetic aminoacyl-lipid II substrates
J. Biol. Chem.
283
34571-34579
2008
Staphylococcus aureus
Salowe, S.P.; Wiltsie, J.; Hawkins, J.C.; Sonatore, L.M.
The catalytic flexibility of tRNAIle-lysidine synthetase can generate alternative tRNA substrates for isoleucyl-tRNA synthetase
J. Biol. Chem.
284
9656-9662
2009
Bacillus subtilis (Q45477), Bacillus subtilis, Bacillus subtilis 168 (Q45477)
Yu, Z.; Takai, K.; Slesarev, A.; Xue, H.; Wong, J.T.
Search for primitive Methanopyrus based on genetic distance between Val- and Ile-tRNA synthetases
J. Mol. Evol.
69
386-394
2009
Methanopyrus kandleri
Baouz, S.; Schmitter, J.M.; Chenoune, L.; Beauvallet, C.; Blanquet, S.; Woisard, A.; Hountondji, C.
Primary structure revision and active site mapping of E. coli isoleucyl-tRNA synthetase by means of maldi mass spectrometry
Open Biochem. J.
3
26-38
2009
Escherichia coli, Escherichia coli EM20031
Paulander, W.; Andersson, D.I.; Maisnier-Patin, S.
Amplification of the gene for isoleucyl-tRNA synthetase facilitates adaptation to the fitness cost of mupirocin resistance in Salmonella enterica
Genetics
185
305-312
2010
Salmonella enterica
Dulic, M.; Cvetesic, N.; Perona, J.J.; Gruic-Sovulj, I.
Partitioning of tRNA-dependent editing between pre- and post-transfer pathways in class I aminoacyl-tRNA synthetases
J. Biol. Chem.
285
23799-23809
2010
Escherichia coli
Istvan, E.S.; Dharia, N.V.; Bopp, S.E.; Gluzman, I.; Winzeler, E.A.; Goldberg, D.E.
Validation of isoleucine utilization targets in Plasmodium falciparum
Proc. Natl. Acad. Sci. USA
108
1627-1632
2011
Plasmodium falciparum
Cestari, I.; Stuart, K.
Inhibition of isoleucyl-tRNA synthetase as a potential treatment for human African Trypanosomiasis
J. Biol. Chem.
288
14256-14263
2013
Trypanosoma brucei
Rettig, J.; Wang, Y.; Schneider, A.; Ochsenreiter, T.
Dual targeting of isoleucyl-tRNA synthetase in Trypanosoma brucei is mediated through alternative trans-splicing
Nucleic Acids Res.
40
1299-1306
2012
Trypanosoma brucei, Trypanosoma brucei 29-13
Dulic, M.; Perona, J.J.; Gruic-Sovulj, I.
Determinants for tRNA-dependent pretransfer editing in the synthetic site of isoleucyl-tRNA synthetase
Biochemistry
53
6189-6198
2014
Escherichia coli (P00956), Escherichia coli
Perli, E.; Giordano, C.; Pisano, A.; Montanari, A.; Campese, A.F.; Reyes, A.; Ghezzi, D.; Nasca, A.; Tuppen, H.A.; Orlandi, M.; Di Micco, P.; Poser, E.; Taylor, R.W.; Colotti, G.; Francisci, S.; Morea, V.; Frontali, L.; Zeviani, M.; dAmati, G.
The isolated carboxy-terminal domain of human mitochondrial leucyl-tRNA synthetase rescues the pathological phenotype of mitochondrial tRNA mutations in human cells
EMBO Mol. Med.
6
169-182
2014
Homo sapiens (Q9NSE4), Homo sapiens
Airas, R.K.
Kinetic analysis of the isoleucyl-tRNA synthetase mechanism the next reaction cycle can start before the previous one ends
FEBS open bio
8
244-255
2018
Escherichia coli (P00956)
Schwartzentruber, J.; Buhas, D.; Majewski, J.; Sasarman, F.; Papillon-Cavanagh, S.; Thiffault, I.; Sheldon, K.; Massicotte, C.; Patry, L.; Simon, M.; Zare, A.; Mckernan, K.; Consortium, F.; Michaud, J.; Boles, R.; Deal, C.; Desilets, V.; Shoubridge, E.; Samuels, M.E.
Mutation in the nuclear-encoded mitochondrial isoleucyl-tRNA synthetase IARS2 in patients with cataracts, growth hormone deficiency with short stature, partial sensorineural deafness, and peripheral neuropathy or with Leigh syndrome
Hum. Mutat.
36
281-281
2015
Homo sapiens (Q9NSE4), Homo sapiens
Cvetesic, N.; Dulic, M.; Bilus, M.; Sostaric, N.; Lenhard, B.; Gruic-Sovulj, I.
Naturally occurring isoleucyl-tRNA synthetase without tRNA-dependent pre-transfer editing
J. Biol. Chem.
291
8618-8631
2016
Escherichia coli (P00956), Escherichia coli, Saccharomyces cerevisiae (P09436), Saccharomyces cerevisiae, Saccharomyces cerevisiae ATCC 204508 / S288c (P09436), Streptomyces griseus
Shepherd, J.; Ibba, M.
Relaxed substrate specificity leads to extensive tRNA mischarging by Streptococcus pneumoniae class I and class II aminoacyl-tRNA synthetases
mBio
5
e01656-14
2014
Streptococcus pneumoniae (Q04JB0), Streptococcus pneumoniae, Streptococcus pneumoniae D39 / NCTC 7466 (Q04JB0)
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