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6.1.1.5: isoleucine-tRNA ligase

This is an abbreviated version!
For detailed information about isoleucine-tRNA ligase, go to the full flat file.

Word Map on EC 6.1.1.5

Reaction

ATP
+
L-isoleucine
 +
tRNAIle
=
AMP
 +
diphosphate
 +
L-isoleucyl-tRNAIle

Synonyms

EcIleRS, IARS2, Ile-tRNA synthetase, IleRS, ileS, ileS1, ileS2, IRS, Isoleucine translase, Isoleucine--tRNA ligase, Isoleucine-transfer RNA ligase, Isoleucine-tRNA synthetase, isoleucyl tRNA synthetase, Isoleucyl-transfer ribonucleate synthetase, Isoleucyl-transfer RNA synthetase, Isoleucyl-tRNA synthetase, mitochondrial isoleucyl-tRNA synthetase, More, mt isoleucyl-tRNA synthetase, mt-IleRS, Mupirocin resistance protein, ScIleRS, SgIleRS, Synthetase, isoleucyl-transfer ribonucleate

ECTree

     6 Ligases
         6.1 Forming carbon-oxygen bonds
             6.1.1 Ligases forming aminoacyl-tRNA and related compounds
                6.1.1.5 isoleucine-tRNA ligase

Crystallization

Crystallization on EC 6.1.1.5 - isoleucine-tRNA ligase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
analysis of the crystal structure with bound muciproin, PDB ID: 1FFY
purified recombinant enzyme cocrystallized with Escherichia coli tRNAIle and inhibitor muciproin, formation of the socalled editing complex, X-ray diffraction structure determination at 2.2 A resolution, and structure analysis
crystallization of CP1 domain alone or in complex with L-valine, X-ray structure determination at 1.8 and 2.0 A resolution respectively, and analysis
crystallization of the 5'-N-[N-(L-isoleucyl)sulfamoyl]adenosine-enzyme complex, and the muciproin-enzyme complex by preparation of enzyme crystals and soaking of the crystals in 0.1 mM 5'-N-[N-(L-isoleucyl)sulfamoyl]adenosine solution, and 1 mM muciproin solution, respectively, X-ray diffraction structure determination at 2.5 A resolution, and analysis
-
IleRS editing domain complexed with the substrate analogues in the pre and post-transfer modes, X-ray diffraction structure determination and analysis at 1.7 A resolution