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0.00016
(s-pA)tRNAPhe
-
-
-
0.00017
(s-pG)tRNAPhe
-
-
-
0.085 - 0.4
2'-deoxyadenosine 5'-triphosphate
0.05 - 0.2
2-Chloroadenosine 5'-triphosphate
1.54
2-deoxyadenosine 5'-triphosphate
-
aminoacylation
2
2-L-naphthylalanine
-
mutant enzyme N412G/T415G/S418C/S437F, at pH 7.6, temperature not specified in the publication
0.3107
2-L-tyrosine
-
at pH 7.5 and 37°C
0.3
3'-deoxadenosine 5'-triphosphate
-
cytoplasmic
0.82 - 1
3'-Deoxyadenosine 5'-triphosphate
0.38 - 0.65
3,4-dihydroxy-L-phenylalanine
1.15
3-L-tyrosine
-
at pH 7.5 and 37°C
923
benzofuranylalanine
-
-
0.012 - 0.13
DL-m-tyrosine
0.00048 - 11
L-phenylalanine
1.4
L-tryptophan
-
mutant enzyme N412G/T415G/S418C/S437F, at pH 7.6, temperature not specified in the publication
0.86
L-Tyr
-
pH 7.2, 37°C, cytosolic enzyme
0.81
N6-methyladenosine 5'-triphosphate
-
aminoacylation
additional information
additional information
-
0.085
2'-deoxyadenosine 5'-triphosphate
-
mitochondrial
0.125
2'-deoxyadenosine 5'-triphosphate
-
-
0.19
2'-deoxyadenosine 5'-triphosphate
-
cytoplasmic
0.4
2'-deoxyadenosine 5'-triphosphate
-
cytoplasmic
0.05
2-Chloroadenosine 5'-triphosphate
-
aminoacylation
0.096
2-Chloroadenosine 5'-triphosphate
-
mitochondrial
0.11
2-Chloroadenosine 5'-triphosphate
-
-
0.16
2-Chloroadenosine 5'-triphosphate
-
cytoplasmic
0.16
2-Chloroadenosine 5'-triphosphate
-
mitochondrial
0.2
2-Chloroadenosine 5'-triphosphate
-
-
0.2
2-Chloroadenosine 5'-triphosphate
-
mitochondrial, 3'-deoxyadenosine 5'-triphosphate
0.2
2-Chloroadenosine 5'-triphosphate
-
cytoplasmic, 2'-deoxyadenosine 5'-triphosphate
0.82
3'-Deoxyadenosine 5'-triphosphate
-
aminoacylation
1
3'-Deoxyadenosine 5'-triphosphate
-
mitochondrial
0.38
3,4-dihydroxy-L-phenylalanine
-
in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C
0.6
3,4-dihydroxy-L-phenylalanine
mitochondrial enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C
0.65
3,4-dihydroxy-L-phenylalanine
cytoplasmic enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C
0.0058
ATP
-
-
0.055
ATP
-
ATP, , mitochondrial, aminoacylation
0.055
ATP
-
mitochondrial
0.06
ATP
-
aminoacylation
0.065
ATP
-
aminoacylation
0.065
ATP
-
mitochondrial
0.08
ATP
-
cytoplasmic, aminoacylation
0.122
ATP
-
recombinant enzyme, pH 7.9, 22°C
0.14
ATP
-
ATP, , cytoplasmic, aminoacylation
0.147
ATP
-
native enzyme, pH 7.9, 22°C
0.2
ATP
-
at pH 7.5 and 37°C
0.225
ATP
-
at pH 7.5 and 37°C
0.62
ATP
-
mitochondrial, ATP-diphosphate exchange
0.7
ATP
-
mitochondrial, ATP-diphosphate exchange
0.84
ATP
-
cytoplasmic, ATP-diphosphate exchange
0.9
ATP
-
cytoplasmic, ATP-diphosphate exchange
2.5
ATP
ATP-diphosphate exchange reaction, recombinant mitochondrial isozyme, pH 7.3, 37°C
0.012
DL-m-tyrosine
mitochondrial enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C
0.13
DL-m-tyrosine
cytoplasmic enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C
0.0018
L-Phe
-
-
0.0042
L-Phe
-
pH 7.2, 37°C, mitochondrial enzyme
0.0103
L-Phe
-
wild-type enzyme
0.0212
L-Phe
-
mutant enzyme with A294G mutation in alpha-subunit
0.0225
L-Phe
-
mutant enzyme with A294G mutation in alpha-subunit and T354W mutation in beta-subunit
0.0261
L-Phe
-
mutant enzyme with A294G mutation in alpha-subunit and E334A mutation in beta-subunit
0.0282
L-Phe
-
mutant enzyme with A294G mutation in alpha-subunit and A356W mutation in beta-subunit
0.03
L-Phe
-
pH 7.2, 37°C, cytosolic enzyme
0.0312
L-Phe
-
mutant enzyme with A294G mutation in alpha-subunit and H265L mutation in beta-subunit
0.0331
L-Phe
-
mutant enzyme with A294G mutation in alpha-subunit and H265A mutation in beta-subunit
0.00048
L-phenylalanine
-
pH 7.8, 60°C
0.00057
L-phenylalanine
mitochondrial chimeric enzyme with implanted editing module from Escherichia coli phenylalanine-tRNA synthetase, at pH 8.5 and 37°C
0.00084
L-phenylalanine
wild type mitochondrial enzyme, at pH 8.5 and 37°C
0.00094
L-phenylalanine
-
recombinant enzyme, pH 7.9, 22°C
0.0012
L-phenylalanine
-
native enzyme, pH 7.9, 22°C
0.0015
L-phenylalanine
-
25°C
0.0015
L-phenylalanine
-
pH 7.8, 70°C
0.0017
L-phenylalanine
-
in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C
0.002
L-phenylalanine
-
wild-type EcPheRS
0.0024
L-phenylalanine
wild type enzyme, at pH 8.5 and 37°C
0.0026
L-phenylalanine
mitochondrial enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C
0.003
L-phenylalanine
-
wild-type ctPheRS
0.0032
L-phenylalanine
cytoplasmic enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C
0.0045
L-phenylalanine
-
mutant A294G EcPheRS
0.0049
L-phenylalanine
-
pH 7.2, 37°C, truncated mutant PheRSDELTAB2A294G
0.005
L-phenylalanine
-
wild-type mtPheRS
0.0053
L-phenylalanine
-
pH 7.2, 37°C, full-length PheRSA294G
0.0056
L-phenylalanine
mutant enzyme H99D, at pH 8.5 and 37°C
0.012
L-phenylalanine
mutant enzyme R117G, at pH 8.5 and 37°C
0.017
L-phenylalanine
-
mutant A333G mtPheRS
0.021
L-phenylalanine
-
at pH 7.5 and 37°C
0.033
L-phenylalanine
ATP-diphosphate exchange reaction, recombinant mitochondrial isozyme, pH 7.3, 37°C
0.0423
L-phenylalanine
-
at pH 7.5 and 37°C
0.048
L-phenylalanine
-
at pH 7.5 and 37°C
0.233
L-phenylalanine
-
mutant G458A ctPheRS
11
L-phenylalanine
-
mutant enzyme N412G/T415G/S418C/S437F, at pH 7.6, temperature not specified in the publication
0.32
L-tyrosine
-
mutant A294G EcPheRS
0.637
L-tyrosine
-
wild-type ctPheRS
0.66
L-tyrosine
-
mutant A333G mtPheRS
1.155
L-tyrosine
-
wild-type mtPheRS
1.9
L-tyrosine
mitochondrial enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C
2.2
L-tyrosine
-
wild-type EcPheRS
3
L-tyrosine
-
mutant G458A ctPheRS
0.00045
Phe
-
-
0.0094
Phe
-
cytoplasmic, aminoacylation
0.011
Phe
-
mitochondrial, aminoacylation
0.012
Phe
-
mitochondrial, aminoacylation
0.013
Phe
-
cytoplasmic, aminoacylation
0.029
Phe
-
mitochondrial, ATP-diphosphate exchange
0.033
Phe
-
cytoplasmic, ATP-diphosphate exchange
0.034
Phe
-
mitochondrial, ATP-diphosphate exchange
0.038
Phe
-
mitochondrial, ATP-diphosphate exchange
0.000066
tRNAPhe
-
-
0.0001
tRNAPhe
-
recombinant heterodimer, pH 8.0, 25°C
0.00011
tRNAPhe
-
recombinant His-tagged heterodimer, pH 8.0, 25°C
0.00012
tRNAPhe
-
substrate from yeast, , aminoacylation
0.00017
tRNAPhe
-
substrate from yeast
0.0002
tRNAPhe
-
substrate from calf liver, , cytoplasmic, aminoacylation
0.00036
tRNAPhe
mutant enzyme T210M, at pH 8.5 and 37°C
0.00042
tRNAPhe
-
cytoplasmic enzyme, aminoacylation
0.00042
tRNAPhe
-
mitochondrial enzyme, calf liver tRNAPhe
0.00052
tRNAPhe
-
substrate from calf liver, , mitochondrial, aminoacylation
0.00058
tRNAPhe
-
substrate from calf liver, , mitochondrial, aminoacylation
0.00065
tRNAPhe
-
substrate from yeast, , aminoacylation
0.00078
tRNAPhe
-
substrate from yeast, , mitochondrial, aminoacylation
0.00094
tRNAPhe
-
at pH 7.5 and 37°C
0.001
tRNAPhe
mutant enzyme D289Y, at pH 8.5 and 37°C
0.0011
tRNAPhe
mutant enzyme P49A, at pH 8.5 and 37°C
0.0012
tRNAPhe
-
at pH 7.5 and 37°C
0.0012
tRNAPhe
wild type enzyme, at pH 8.5 and 37°C
0.0012
tRNAPhe
mutant enzyme R387Q, at pH 8.5 and 37°C
0.0021
tRNAPhe
mutant enzyme R383C, at pH 8.5 and 37°C
0.0022
tRNAPhe
-
pH 7.2, 37°C, full-length PheRSA294G, substrate from Saccharomyces cerevisiae
0.0025
tRNAPhe
-
pH 7.2, 37°C, truncated mutant PheRSDELTAB2A294G, substrate from Escherichia coli
0.0027
tRNAPhe
-
pH 7.2, 37°C, full-length PheRSA294G, substrate from Escherichia coli
0.0032
tRNAPhe
-
substrate from E. coli
0.0041
tRNAPhe
-
pH 7.2, 37°C, truncated mutant PheRSDELTAB2A294G, substrate from Saccharomyces cerevisiae
0.018
tRNAPhe
aminoacylation reaction, recombinant mitochondrial isozyme, pH 7.5, 37°C
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
Km value for aminoacylation of tRNAPhe-C-C-A or tRNAPhe-C-C-A(3'NH2) with various amino acids
-
additional information
additional information
-
Km-value for tRNA mutants
-
additional information
additional information
-
Km-values for mutant yeast tRNAPhe transcripts
-
additional information
additional information
-
Km-values for mutant yeast tRNAPhe transcripts
-
additional information
additional information
-
negative cooperativity exists in the binding of all substrates
-
additional information
additional information
-
kinetics, binding energies in the active conformation
-
additional information
additional information
-
aminoacylation kinetics with Phe and Tyr
-
additional information
additional information
-
full-length PheRSA294G and truncated mutant PheRSDELTAB2A294G show comparable kinetics for in vitro aminoacylation, kinetics, overview
-