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6.1.1.19: arginine-tRNA ligase

This is an abbreviated version!
For detailed information about arginine-tRNA ligase, go to the full flat file.

Word Map on EC 6.1.1.19

Reaction

ATP
+
L-arginine
 +
tRNAArg
=
AMP
 +
diphosphate
 +
L-arginyl-tRNAArg

Synonyms

Arg-tRNA synthetase, Arginine translase, Arginine--tRNA ligase, Arginine-tRNA synthetase, Arginyl transfer ribonucleic acid synthetase, Arginyl-transfer RNA synthetase, Arginyl-tRNA synthetase, arginyl–tRNA synthetase, ArgRS, ArgS2, MtArgRS, RARS, RARS2, RRS, Synthetase, arginyl-transfer ribonucleate

ECTree

     6 Ligases
         6.1 Forming carbon-oxygen bonds
             6.1.1 Ligases forming aminoacyl-tRNA and related compounds
                6.1.1.19 arginine-tRNA ligase

Cloned

Cloned on EC 6.1.1.19 - arginine-tRNA ligase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
arginyl tRNA synthetase and an N-terminally truncated derivative with a deletion of the 73 N-terminal amino acids, expression in Escherichia coli
-
construction of pMFT7H6-hcArgRS by inserting the full-length hcArgRS gene into the gap between NcoI and BamH1 of vector pMFT7H6. Expression of His-tagged wild-type full-length and truncated mutant enzymes in Escherichia coli strain BL21-CodonPlus (DE3)-RIL
-
deduced amino acid sequence shows 87.7% identity to the CHO enzyme and 37.7% identity to the homologous Escherichia coli enzyme
-
expressed in Escherichia coli
-
expressed in Escherichia coli BL21 Codon Plus RP cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli Rosetta(DE3) cells
expressed in Escherichia coli strain MT 102
-
expression in Escherichia coli of a His-tagged recombinant enzyme, the enzyme has 73% homology with respect to Bacillus subtilis ArgRS, and 22% with respect to Escherichia coli
-
expression of 26 lethal mutants in Escherichia coli
-
expression of GST-tagged MtArgRS in Escherichia coli strain BL21(DE3), co-expression of MtArgRS with seryl-tRNA synthetase, MtSerRS, EC 6.1.1.11, from in the Saccharomyces cerevisiae two-hybrid system using yeast strain MaV203
-
expression of the native and mutant enzymes in Saccharomyces cerevisiae
-
expression of the wild type and K116G mutant in Escherichia coli
-
full-length enzyme and mutant DELTA1-72 tagged with green fluorescence protein, expression in 293 T cells, exclusively in cytosol
-
gene argS, DNA and amino acid sequence determination and analysis, expression of N-terminally HIS-/thioredoxin-tagged enzyme in Escherichia coli strain BL21
into the TOPO vector and subsequently into the vector pET28c for expression in Escherichia coli BL21DE3codon+ cells
into the vector pET32a for expression in Escherichia coli BL21 cells
overexpressed in Escherichia coli under the control of the T7 promoter
overexpression in Escherichia coli
-
overexpression in Escherichia coli of wild type and several mutant enzymes
overexpression of the wild type and the argS1 mutant enzymes in Escherichia coli
-