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6.1.1.18: glutamine-tRNA ligase

This is an abbreviated version!
For detailed information about glutamine-tRNA ligase, go to the full flat file.

Word Map on EC 6.1.1.18

Reaction

ATP
+
L-glutamine
 +
tRNAGln
=
AMP
 +
diphosphate
 +
L-glutaminyl-tRNAGln

Synonyms

class I glutaminyl-tRNA synthetase, cytosolic glutaminyl-tRNA synthetase, Gln-RS, Gln4, GlnRS, Glutamine translase, Glutamine--tRNA ligase, Glutamine-tRNA synthetase, glutaminyl tRNA synthetase, Glutaminyl-transfer ribonucleate synthetase, Glutaminyl-transfer RNA synthetase, Glutaminyl-tRNA synthetase, glutaminyltRNA synthetase, glutamyl/glutaminyl-tRNA synthetase, QARS, QRS, Synthetase, glutaminyl-transfer ribonucleate, Vegetative specific protein H4

ECTree

     6 Ligases
         6.1 Forming carbon-oxygen bonds
             6.1.1 Ligases forming aminoacyl-tRNA and related compounds
                6.1.1.18 glutamine-tRNA ligase

Cloned

Cloned on EC 6.1.1.18 - glutamine-tRNA ligase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA sequence determination and analysis, enzyme contains a N-terminal lysine-rich sequence KPKKKKEK, that may function as a nuclear targetng signal as well as in regulation of gene expression, since the 8 amino acid peptide derived from the motif interacts with DNA and changes the DNA molecule form from B to Z form
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Saccharomyces cerevisiae strain BCY123
expression of full length and C-terminal truncated GlnRS, lacking the the Yqey domain, and of the isolated Yqey protein, in Escherichia coli strain ER2566
expression of His-tagged GlnRS mutants in strain BL21-DE3
-
expression of the His-tagged chimeric mutant enzyme in Escherichia coli strain BL21(DE3) or the temperature sensitive strain JP1449(DE3)
-
gene GLN4, phylogenetic analysis
gene glnS, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21
gene QARS, genotyping, recombinant expression of codon-optimized His-tagged wild-type and mutant enzymes in Escherichia coli
gene QARS, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain Rosetta (DE3)pLysS
overexpression in Escherichia coli
-
overexpression of various Myc-tagged enzyme mutants in human embryonic kidney 293 cells
-
recombinant expression of His6-tagged wild-type full-length enzyme and truncated mutant enzymes in Escherichia coli strain BL21(DE3), optimization of expression conditions
recombinant expression of the enzyme in Escherichia coli
the vector pET28a is used, Escherichia coli JP1449DE3 cells are used
-
transient expression of the p.Lys496stop mutant in neuroblastoma 2A cells reveals diminished and aberrantly aggregated expression