5.5.1.9: Cycloeucalenol cycloisomerase

This is an abbreviated version!
For detailed information about Cycloeucalenol cycloisomerase, go to the full flat file.

Reaction

Synonyms

Atcpi, Cycloeucalenol isomerase, Cycloeucalenol--obtusifoliol isomerase, Cycloeucalenol-obtusifoliol isomerase, Cyclopropyl sterol isomerase, Isomerase, cycloeucalenol-obtusifoliol

ECTree

     5 Isomerases

         5.5 Intramolecular lyases

             5.5.1 Intramolecular lyases (only sub-subclass identified to date)

                5.5.1.9 Cycloeucalenol cycloisomerase

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Results	in table
17	AA Sequence
1	CAS Registry Number
2	Cloned(Commentary)
18	Inhibitors
12	KM Value [mM]
7	Localization
2	Natural Substrates/ Products (Substrates)
17	Organism
8	Pathway
33	Protein Variants
4	Reaction
1	Reaction Type
17	Reference
12	Source Tissue
21	Substrates and Products (Substrate)
6	Synonyms
1	Systematic Name

Engineering

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Engineering on EC 5.5.1.9 - Cycloeucalenol cycloisomerase

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

D260E

Arabidopsis thaliana

-

cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 92%, wild-type: 100%. Relative activity: 135% (wild-type: 100%). Km (cycloeucalenol) increased compared to wild-type. Mutant shows 6% activity with 24-methylene-24-dihydro-cycloartenol (wild-type 0%)

729167

D260H

Arabidopsis thaliana

-

cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 61%, wild-type: 100%. Relative activity: 5.2% (wild-type: 100%). Km (cycloeucalenol) decreased compared to wild-type

729167

D260V

Arabidopsis thaliana

-

cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 0%, wild-type: 100%. Relative activity: below 1.5% (wild-type: 100%)

729167

D260Y

Arabidopsis thaliana

-

cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 22%, wild-type: 100%. Relative activity: 0.7% (wild-type: 100%)

729167

E29D

Arabidopsis thaliana

-

cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 65%, wild-type: 100%. Relative activity: 3.8% (wild-type: 100%). Km (cycloeucalenol) decreased compared to wild-type

729167

E29H

Arabidopsis thaliana

-

cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 33%, wild-type: 100%. Relative activity: 3.5% (wild-type: 100%). Km (cycloeucalenol) decreased compared to wild-type

729167

E29K

2 entries

E29V

Arabidopsis thaliana

-

cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 0%, wild-type: 100%. Relative activity: below 0.5% (wild-type: 100%)

729167

E54V

Arabidopsis thaliana

-

cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 94%, wild-type: 100%

729167

E58V

Arabidopsis thaliana

-

cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 91%, wild-type: 100%

729167

E60V

Arabidopsis thaliana

-

cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 83%, wild-type: 100%

729167

F111L

Arabidopsis thaliana

-

cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 88%, wild-type: 100%. Relative activity: 31% (wild-type: 100%). Km (cycloeucalenol) increased compared to wild-type

729167

F116L

Arabidopsis thaliana

-

cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 37%, wild-type: 100%. Relative activity: 4.7% (wild-type: 100%)

729167

F117L

Arabidopsis thaliana

-

cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 89%, wild-type: 100%. Relative activity: 28% (wild-type: 100%). Km (cycloeucalenol) increased compared to wild-type

729167

G108A

Arabidopsis thaliana

-

cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 92%, wild-type: 100%. Relative activity: 90% (wild-type: 100%)

729167

G108E

2 entries

G108V

Arabidopsis thaliana

-

cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 6%, wild-type: 100%. Relative activity: 1.7% (wild-type: 100%)

729167

G28E

2 entries

G28V

Arabidopsis thaliana

-

cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 46%, wild-type: 100%. Relative activity: 1.6% (wild-type: 100%)

729167

H114L

Arabidopsis thaliana

-

cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 85%, wild-type: 100%. Relative activity: 5.2% (wild-type: 100%)

729167

N109A

Arabidopsis thaliana

-

cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 76%, wild-type: 100%. Relative activity: 4.6% (wild-type: 100%)

729167

T113A

Arabidopsis thaliana

-

cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 69%, wild-type: 100%. Relative activity: 7.2% (wild-type: 100%). Km (cycloeucalenol) increased compared to wild-type

729167

T113I

2 entries

T113S

Arabidopsis thaliana

-

cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 98%, wild-type: 100%. Relative activity: 73% (wild-type: 100%). Km (cycloeucalenol) increased compared to wild-type

729167

W112E

Arabidopsis thaliana

-

cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 77%, wild-type: 100%. Relative activity: 5.8% (wild-type: 100%)

729167

W112K

Arabidopsis thaliana

-

cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 47%, wild-type: 100%. Relative activity: 1.8% (wild-type: 100%)

729167

W112L

Arabidopsis thaliana

-

cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 86%, wild-type: 100%. Relative activity: 49% (wild-type: 100%). Km (cycloeucalenol) decreased compared to wild-type. Mutant shows 69% activity with 24-methylene-24-dihydro-cycloartenol (wild-type 0%), 10% activity with cycloartenol (wild-type 0%). 32% activity with cycloartanol (wild-type 0%), 15% activity with 4beta-methyl-24-dihydro-cycloeucalenol (wild-type 0%)

729167

Y110L

Arabidopsis thaliana

-

cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 64%, wild-type: 100%. Relative activity: 2.8% (wild-type: 100%)

729167

Y115L

Arabidopsis thaliana

-

cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 83%, wild-type: 100%. Relative activity: 4.3% (wild-type: 100%)

729167

E29K

Arabidopsis thaliana

-

cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 1.7%, wild-type: 100%. Relative activity: below 0.5% (wild-type: 100%)

729167

E29K

Arabidopsis thaliana

-

in vivo conversion of cycloeucalenol into ergosterol and in vitro CPI activity in Saccharomyces cerevisiae erg7 transformants carrying Arabidopsis thaliana mutant CPI: 1.7% relative ergosterol synthesis (wild-type: 100%), below 0.5% in vitro microsomal CPI activity (wild-type: 100%)

729167

G108E

Arabidopsis thaliana

-

cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 0%, wild-type: 100%. Relative activity: below 0.5% (wild-type: 100%). Km (cycloeucalenol) increased compared to wild-type

729167

G108E

Arabidopsis thaliana

-

in vivo conversion of cycloeucalenol into ergosterol and in vitro CPI activity in Saccharomyces cerevisiae erg7 transformants carrying Arabidopsis thaliana mutant CPI: 0% relative ergosterol synthesis (wild-type: 100%), below 0.5% in vitro microsomal CPI activity (wild-type: 100%)

729167

G28E

Arabidopsis thaliana

-

cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 15%, wild-type: 100%. Relative activity: 1% (wild-type: 100%)

729167

G28E

Arabidopsis thaliana

-

in vivo conversion of cycloeucalenol into ergosterol and in vitro CPI activity in Saccharomyces cerevisiae erg7 transformants carrying Arabidopsis thaliana mutant CPI: 15% relative ergosterol synthesis (wild-type: 100%), 1% in vitro microsomal CPI activity (wild-type: 100%)

729167

T113I

Arabidopsis thaliana

-

cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 66%, wild-type: 100%. Relative activity: 3.1% (wild-type: 100%)

729167

T113I

Arabidopsis thaliana

-

in vivo conversion of cycloeucalenol into ergosterol and in vitro CPI activity in Saccharomyces cerevisiae erg7 transformants carrying Arabidopsis thaliana mutant CPI: 66% relative ergosterol synthesis (wild-type: 100%), below 2.4% in vitro microsomal CPI activity (wild-type: 100%)

729167