5.5.1.6: chalcone isomerase

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For detailed information about chalcone isomerase, go to the full flat file.

Word Map on EC 5.5.1.6

5.5.1.6

flavonoid

anthocyanins

dihydroflavonol

ammonia-lyase

naringenin

phenylpropanoids

anthocyanidin

4-reductase

cinnamate

isoflavone

flavone

4-hydroxylase

isoflavonoids

3\'-hydroxylase

3-o-glucosyltransferase

r2r3-myb

4-coumarate:coa

leucoanthocyanidin

4-coumarate

3',5'-hydroxylase

agriculture

petroselinum

2s-naringenin

2s-flavanones

analysis

lindemuthianum

4.3.1.5

synthesis

Reaction

Synonyms

Chalcone isomerase, chalcone isomerase-like protein, Chalcone-flavanone isomerase, CHI, Chi-1, Chi1, CHI1A, CHI1B1, CHI1B2, CHI2, CHI2B1, CHI2B2, Chi3, CHI3A, CHI3A1, CHI3A2, CHI3B, CHI3B1, CHI3C1, CHI3C2, CHI4, CHI4A, CHI4B, CHIL, CHIL1, CHIL2, EFP, Isomerase, chalcone, MpCHI, SlCHI1, taxifolin-isomerizing enzyme, TRANSPARENT TESTA 5 protein, TT5, type IV CHI protein

ECTree

5 Isomerases

5.5 Intramolecular lyases

5.5.1 Intramolecular lyases (only sub-subclass identified to date)

5.5.1.6 chalcone isomerase

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Results	in table
1	Activating Compound
264	AA Sequence
5	Application
1	CAS Registry Number
36	Cloned(Commentary)
4	Crystallization (Commentary)
9	Expression
18	General Information
1	General Stability
48	Inhibitors
34	kcat/KM [mM/s]
73	KM Value [mM]
17	Localization
22	Molecular Weight [Da]
30	Natural Substrates/ Products (Substrates)
170	Organism
10	Pathway
54	PDB ID
9	pH Optimum
4	pH Range
3	pI Value
1	Posttranslational Modification
20	Protein Variants
18	Purification (Commentary)
2	Reaction
1	Reaction Type
103	Reference
96	Source Tissue
7	Specific Activity [micromol/min/mg]
3	Storage Stability
114	Substrates and Products (Substrate)
28	Subunits
89	Synonyms
1	Systematic Name
5	Temperature Optimum [°C]
1	Temperature Range [°C]
1	Temperature Stability [°C]
65	Turnover Number [1/s]

Purification

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Purification on EC 5.5.1.6 - chalcone isomerase

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7 entries

ammonium sulfate precipitation, DEAE-Sephacel column chromatography, phenyl Sepharose column chromatography, Mono Q column chromatography, Superdex 200 gel filtration, and phenyl Superose gel filtration

Eubacterium ramulus

V9P0A9

748106

ammonium sulfate precipitation, DEAE-Sepharose column chromatography, butyl-Sepharose column chromatography, and Superdex 200 gel filtration

Eubacterium ramulus

V9P0A9

746629

Ni-NTA affinity resin column chromatography

Arabidopsis thaliana

748323

Ni-NTA column chromatography and Superdex 200 gel filtration

Deschampsia antarctica

G4U3G8

749122

partial, 3 isoenzymes

Glycyrrhiza echinata

653468

recombinant

Glycine max

Q53B70, Q53B74, Q93XE6

663135

recombinant enzyme

2 entries

recombinant His-tagged CHI from Escherichia coli strain BL21 (DE3)

Ginkgo biloba

716505