5.4.99.11: isomaltulose synthase

This is an abbreviated version!
For detailed information about isomaltulose synthase, go to the full flat file.

Word Map on EC 5.4.99.11

5.4.99.11

synthesis

erwinia

rhapontici

palatinose

protaminobacter

2.4.1.1

pantoea

1,4-alpha-d-glucan:orthophosphate

molasses

plymuthica

food industry

vacuole-targeted

dispersa

whiteflies

bemisia

honeydew

Reaction

6-O-alpha-D-glucopyranosyl-D-fructofuranose

Synonyms

alpha-Glucosyltransferase, alpha-glycosyl transferase, Avin_08330, AZOG, EC 5.4.99.10, esi, isomaltulose synthase, Isomaltulose synthetase, MutB, NX-5, PalI, Pall, PdSIase, SIase, SmuA, Sucrose 6-glucosylmutase, Sucrose alpha-glucosyltransferase, sucrose isomerase, Sucrose mutase, Synthase, isomaltulose, Trehalulose synthase

ECTree

5 Isomerases

5.4 Intramolecular transferases

5.4.99 Transferring other groups

5.4.99.11 isomaltulose synthase

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Results	in table
1	Activating Compound
26	AA Sequence
56	Application
1	CAS Registry Number
30	Cloned(Commentary)
12	Crystallization (Commentary)
31	General Information
1	General Stability
40	Inhibitors
18	kcat/KM [mM/s]
3	Ki Value [mM]
49	KM Value [mM]
5	Localization
11	Metals/Ions
18	Molecular Weight [Da]
52	Natural Substrates/ Products (Substrates)
122	Organism
22	PDB ID
23	pH Optimum
11	pH Range
9	pH Stability
12	pI Value
2	Posttranslational Modification
94	Protein Variants
22	Purification (Commentary)
5	Reaction
3	Reaction Type
87	Reference
3	Source Tissue
49	Specific Activity [micromol/min/mg]
7	Storage Stability
140	Substrates and Products (Substrate)
34	Subunits
143	Synonyms
1	Systematic Name
35	Temperature Optimum [°C]
8	Temperature Range [°C]
18	Temperature Stability [°C]
27	Turnover Number [1/s]

Purification

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Purification on EC 5.4.99.11 - isomaltulose synthase

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5 entries

ammonium sulfate precipitation, DEAE-Sepharose column chromatography, SP-Sepharose column chromatography, and Sephacryl S-300 gel filtration

Erwinia rhapontici

714486

expression in Escherichia coli

Paraburkholderia acidicola

660622

native enzyme 17.9fold by adsorption on a chromatographic resin

Erwinia sp.

726847

Ni-NTA column chromatography

Erwinia rhapontici

D9MPF2

713794

Ni-NTA column chromatography, gel filtration

Enterobacter sp. FMB-1

B5ABD8

704227

purification and immobilization in a single step

Serratia plymuthica

3470

recombinant enzyme from Escherichia coli strain BL21(DE3)

2 entries

recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration

Pantoea dispersa

Q6XNK6

748342

recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatograhy and gel filtration

Erwinia rhapontici

728625

recombinant His6-tagged enzyme from Escherichia coli by nickle affinity chromatography and gel filtration

Pectobacterium carotovorum

S5YEW8

727383

recombinant protein

2 entries

recombinant wild-type and mutant enzymes

Rhizobium sp.

M1E1F6

726588

recombinant wild-type enzyme and mutants E175N, K576D, and E175N/K576D from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation, dialysis, and cation exchange chromatography, to homogeneity

Serratia plymuthica

D0VX20

749077

using subsequently a Sephadex G-25, a DEAE-cellulose and a Sephadex G-75 column

Erwinia rhapontici

690512