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5.4.99.11: isomaltulose synthase

This is an abbreviated version!
For detailed information about isomaltulose synthase, go to the full flat file.

Word Map on EC 5.4.99.11

Reaction

sucrose
=
6-O-alpha-D-glucopyranosyl-D-fructofuranose

Synonyms

alpha-Glucosyltransferase, alpha-glycosyl transferase, Avin_08330, AZOG, EC 5.4.99.10, esi, isomaltulose synthase, Isomaltulose synthetase, MutB, NX-5, PalI, Pall, PdSIase, SIase, SmuA, Sucrose 6-glucosylmutase, Sucrose alpha-glucosyltransferase, sucrose isomerase, Sucrose mutase, Synthase, isomaltulose, Trehalulose synthase

ECTree

     5 Isomerases
         5.4 Intramolecular transferases
             5.4.99 Transferring other groups
                5.4.99.11 isomaltulose synthase

Cloned

Cloned on EC 5.4.99.11 - isomaltulose synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning and expression in Escherichia coli strain DH10B, expression on cell surface of Saccharomyces cerevisiae strain FMB-1
-
cloning of the PdSIase gene into the expression vector pET28a, functional recombinant expression in Yarrowia lipolytica strain CGMCC7326 on the cell surface using the cell wall protein Pir1 as an anchor protein, and recombinant expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3)
DNA and amino acid sequence determination and analysis, sequence comparisons, functional expression of His6-tagged enzyme in Escherichia coli
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli JM109 cells
Paraburkholderia acidicola
expressed in sugarcane (Saccharum sp.) cultivar Q117 embryogenic callus
Paraburkholderia acidicola
expression in Escherichia coli
expression in Escherichia coli, with His-tag
expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
-
gene Avin_08330, sequence comparisons and phylogenetic tree, recombinant expression of wild-type AZOG enzyme and chimeric mutants AZF3 and AZF5 in Escherichia coli strain BL21(DE3)
gene esi, sequence comparisons and phylogenetic tree, recombinant expression chimeric mutants AZF3 and AZF5 in Escherichia coli strain BL21(DE3)
gene mutB, DNA and amino acid sequence determination and analysis, phylogenetic analysis, cloning and expression of wild-type and insertional disruption mutant in Escherichia coli strain BL21 (DE3)
gene mutB, recombinant expression of wild-type and mutant enzymes
gene smuA, DNA and amino acid sequence determination and analysis, phylogenetic analysis, cloning of full-length gene including the periplasmic signal sequence and expression of wild-type and insertional disruption mutant in Escherichia coli strain BL21 (DE3)
gene smuA, sequence comparison, expression of wild-type and mutant enzymes
gene smuA, synthetic gene, sequence comparisons, recombinant expression of codon-optimized wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain JM109
recombinant enzyme expression
recombinant enzyme expression from tac vector
recombinant enzyme expression, Protaminobacter sucrose isomerase is totally susceptible to inactivation and cannot be solubly expressed with a tac vector either but may fold correctly and is effectively expressed from the L-rhamnose-inducible vector
-
recombinant expression in Escherichia coli strain BL21(DE3) periplasmic space using vector pET22b-palI, usage of untreated cane molasses and corn steep liquor for Escherichia coli fermentation