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5.4.2.8: phosphomannomutase

This is an abbreviated version!
For detailed information about phosphomannomutase, go to the full flat file.

Word Map on EC 5.4.2.8

Reaction

alpha-D-glucose 1-phosphate
=
D-glucose 6-phosphate

Synonyms

alpha-D-phosphohexomutase, alpha-phosphomannomutase1, EC 2.7.5.7, IMP-sensitive glucose-1,6-bisphosphatase, ManB, ORF17, PGM/PMM, PGM2, PgmG, phosphoglucomutase/phosphomannomutase, Phosphohexomutase, phosphomannomutase, phosphomannomutase 1, phosphomannomutase 2, phosphomannomutase/phosphoglucomutase, phosphomannomutase2, Phosphomannose mutase, Phosphomutase, mannose, PMM, PMM-1, PMM-2, PMM-A1, PMM-A2, PMM-B1, PMM-B2, PMM-D1, PMM-D2, PMM/PGM, PMM1, PMM2, PMMH-22, SP-2, SSO0207

ECTree

     5 Isomerases
         5.4 Intramolecular transferases
             5.4.2 Phosphotransferases (phosphomutases)
                5.4.2.8 phosphomannomutase

Purification

Purification on EC 5.4.2.8 - phosphomannomutase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, ion exchange column chromatography, and Sephacryl S-100 gel filtration
-
ammonium sulfate, Sephadex G-25, KM-cellulose, partially purified
-
cell extracts are purified by His-tag agarose beads
-
DEAE-Sepharose column chromatography and Superdex 75 gel filtration
heat treatment 85°C, 20 min, anion-exchange, hydrophobic and gel filtration column chromatography
-
Ni-NTA column chromatography
-
Ni-Sepharose column chromatography
-
nickel affinity chromatography column chromatography
nickel-affinity column chromatography
-
nickel-chelating resin chromatography
-
partial, alginate-producing strain V388
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3), removal of the His-tag, purification by nickel affinity chromatography and dialysis
-
recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography and gel filtration, removal of the His-tag by TEV protease
recombinant PMM/PGM
-
recombinant PMM2, polyethylene glycol, DEAE-Sepharose, Q-Sepharose
-
Talon metal affinity resin chromatography
-