5.4.2.7: phosphopentomutase
This is an abbreviated version!
For detailed information about phosphopentomutase, go to the full flat file.
Word Map on EC 5.4.2.7
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5.4.2.7
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nucleoside
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purine
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5-phosphate
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phosphoglucomutase
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deoxyriboaldolase
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2-deoxyribose
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ribose-5-phosphate
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nucleobase
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phosphorylases
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deoxyribonucleosides
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2'-deoxyribonucleoside
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ribokinase
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1,6-diphosphate
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phosphorolysis
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phosphomutases
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analysis
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biotechnology
- 5.4.2.7
- nucleoside
- purine
- 5-phosphate
- phosphoglucomutase
- deoxyriboaldolase
- 2-deoxyribose
- ribose-5-phosphate
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nucleobase
- phosphorylases
- deoxyribonucleosides
- 2'-deoxyribonucleoside
- ribokinase
- 1,6-diphosphate
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phosphorolysis
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phosphomutases
- analysis
- biotechnology
Reaction
Synonyms
alpha-D-Glucose-1,6-bisphosphate:deoxy-D-ribose-1-phosphate phosphotransferase, alpha-D-ribose1,5-phosphomutase, deoB, Deoxyribomutase, Deoxyribose phosphomutase, EC 2.7.5.6, PGM3, Phosphodeoxyribomutase, Phosphomutase, deoxyribose, phosphopentomutase, Phosphoribomutase, PPM, PPMase
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Purification
Purification on EC 5.4.2.7 - phosphopentomutase
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IMAC Ni-charged resin column chromatography, and Superdex 200 gel filtration
recombinant His-tagged Pgm1, Pgm2, and Pgm3 from Escherichia coli strain BL21 (DE3)
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recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, His-tag removal by thrombin cleavage, followed by gel filtration
recombinant PPM, Toyopearl HW-55, DEAE-Toyopearl, Butyl-Toyopearl, Sephacryl S-330
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