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5.4.2.7: phosphopentomutase

This is an abbreviated version!
For detailed information about phosphopentomutase, go to the full flat file.

Word Map on EC 5.4.2.7

Reaction

alpha-D-ribose 1-phosphate
=
D-ribose 5-phosphate

Synonyms

alpha-D-Glucose-1,6-bisphosphate:deoxy-D-ribose-1-phosphate phosphotransferase, alpha-D-ribose1,5-phosphomutase, deoB, Deoxyribomutase, Deoxyribose phosphomutase, EC 2.7.5.6, PGM3, Phosphodeoxyribomutase, Phosphomutase, deoxyribose, phosphopentomutase, Phosphoribomutase, PPM, PPMase

ECTree

     5 Isomerases
         5.4 Intramolecular transferases
             5.4.2 Phosphotransferases (phosphomutases)
                5.4.2.7 phosphopentomutase

Purification

Purification on EC 5.4.2.7 - phosphopentomutase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
HisTrap column chromatography
-
HisTrap HP column chromatography and Superdex 200 gel filtration
-
IMAC Ni-charged resin column chromatography, and Superdex 200 gel filtration
multiple forms: I, II, II-1 and III-2
-
recombinant His-tagged Pgm1, Pgm2, and Pgm3 from Escherichia coli strain BL21 (DE3)
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, His-tag removal by thrombin cleavage, followed by gel filtration
recombinant PPM, Toyopearl HW-55, DEAE-Toyopearl, Butyl-Toyopearl, Sephacryl S-330
-