5.4.2.6: beta-Phosphoglucomutase

This is an abbreviated version!
For detailed information about beta-Phosphoglucomutase, go to the full flat file.

Reaction

Synonyms

beta-PGM, beta-phosphoglucomutase, betaPGM, PgcM, Phosphomutase, beta-glucose, PsPGM, Py04_1503, TM1254, YcjU

ECTree

     5 Isomerases

         5.4 Intramolecular transferases

             5.4.2 Phosphotransferases (phosphomutases)

                5.4.2.6 beta-Phosphoglucomutase

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Results	in table
11	Activating Compound
3272	AA Sequence
1	Application
1	CAS Registry Number
13	Cloned(Commentary)
8	Crystallization (Commentary)
10	General Information
1	General Stability
34	Inhibitors
4	kcat/KM [mM/s]
1	Ki Value [mM]
20	KM Value [mM]
30	Metals/Ions
14	Molecular Weight [Da]
21	Natural Substrates/ Products (Substrates)
71	Organism
5	Pathway
72	PDB ID
8	pH Optimum
4	pH Range
2	pH Stability
1	Posttranslational Modification
15	Protein Variants
15	Purification (Commentary)
5	Reaction
2	Reaction Type
32	Reference
2	Source Tissue
11	Specific Activity [micromol/min/mg]
3	Storage Stability
53	Substrates and Products (Substrate)
11	Subunits
26	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
1	Temperature Range [°C]
9	Temperature Stability [°C]
8	Turnover Number [1/s]

Crystallization

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Crystallization on EC 5.4.2.6 - beta-Phosphoglucomutase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

beta-PGM is transferred to 1 mM HEPES, pH 7.5, 10 mM MgCl2 and 0.1 mM dithiothreitol, final beta-PGM concentration at 15 mg/ml, crystals are obtained from either 150 mM ammonium acetate, 100 mM trisodium citrate dihydrate, pH 4.5, 25% polyethylene glycol 4000 or 100 mM ammonium fluoride, 16% polyethylene glycol 3350 by hanging-drop vapor diffusion, crystals of selenium methionyl-substituted beta-PGM diffract to 2.3 A

Lactococcus lactis

-

649190

both in complex with inhibitor alpha-D-galactose 1-phosphate or substrate beta-D-glucose 1-phosphate

Lactococcus lactis

P71447

661947

characterization of the solution structure of the MgF3- complex bound to the enzyme active site

Lactococcus lactis

P71447

682512

crystals of phosphorylated native beta-PGM are grown at pH 7.5 in the presence of 5 M Mg2+ using the vapor-diffusion method with hanging drop geometry, 100 mM ammonium fluoride and 16% polyethylene glycol 3350 are used as precipitating agents, crystals diffrakt to 2.3 A

Lactococcus lactis

P71447

650174

in complex with Mg2+

Lactococcus lactis

P71447

661214

purified recombinant enzyme in complex with transition-state analogue fluoromagnesate and glucose 6-phosphate or ground-state analogue fluoroaluminate and glucose 6-phosphate, sitting drop vapour diffusion method, mixing of 15 mg/mL 50 mM K+ HEPES, pH 7.2, 5 mM MgCl2, 1 mM azide, 0.1 mM DTT, 10 mM NH4F, and 10 mM BeCl2 1:1 with the precipitant containing 26-30% w/v PEG 4000, 200 mM Na acetate, and 100 mM Tris, pH 7.5, X-ray diffraction structure determination and analysis at 1.65 A resolution, molecular replacement

Lactococcus lactis

-

728684

in complex with alpha-fluorophosphonates, sitting drop vapor diffusion method, using 27-32% (w/v) PEG 4000, and 50-75 mM magnesium acetate

Lactococcus lactis subsp. lactis

P71447

749166

sitting drop vapor diffusion method, using 0.1 M trisodium citrate pH 5.0 containing 1.8 M ammonium sulfate

Thermotoga maritima

-

701504