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5.4.2.2: phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)

This is an abbreviated version!
For detailed information about phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent), go to the full flat file.

Word Map on EC 5.4.2.2

Reaction

alpha-D-glucose 1-phosphate
=
D-glucose 6-phosphate

Synonyms

alpha phosphoglucomutase, alpha-Pgm, alpha-phosphoglucomutase, alpha-phosphoglucomutase 1, alpha-phosphoglucose mutase, cPGM, cytosolic phosphoglucomutase, EC 2.7.5.1, Glucose phosphomutase, PGM, PGM-I, PGM-II, PGM/PMM, PGM1, PGM2, PGM3, PgmA, PgmG, phospho-glucomutase, phosphoglucomutase, phosphoglucomutase 1, phosphoglucomutase/phosphomannomutase, phosphoglucomutase1, Phosphoglucose mutase, Phosphohexomutase, phosphomannomutase/phosphoglucomutase, Phosphomutase, glucose, plastidic phosphoglucomutase, PMM/PGM, pPGM, SP-1, SpgM, SSO0207, XanA, YMR278w protein

ECTree

     5 Isomerases
         5.4 Intramolecular transferases
             5.4.2 Phosphotransferases (phosphomutases)
                5.4.2.2 phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)

Cloned

Cloned on EC 5.4.2.2 - phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned and homologously overexpressed in Lactococcus lactis subsp. cremoris strain NZ9000, introducing 6xHis-tag
expressed as phosphomannomutase/phosphoglucomutase fusion protein in Escherichia coli Transetta (DE3) cells
-
expressed in Eascherichia coli and Saccharomyces cerevisiae BY4741 wild type and pgm1DELTA/pgm2DELTA mutant strain
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expressed in Escherichia coli
expressed in Escherichia coli BL21 and Rosetta(DE3) cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli Rosetta (DE3) cells
expressed in Saccharomyces cerevisiae strain JF645
-
expression in Agrobacterium tumefaciens
expression in Escherichia coli
expression of cytosolic and plastidial isozymes in Nicotiana tabacum cv. Xanthi using the cauliflower mosaic virus 35S promoter. The transgenic plants expressing Arabidopsis plastidial PGM show 3.5-8.2fold higher enzyme activity compared to the wild-type, and leaf starch and sucrose contents increase 2.3-3.2fold and 1.3-1.4fold, respectively over wild-type levels, while transgenic plants expressing Arabidopsis cytosolic PGM show a 2.1-3.4fold increase in PGM activity over wild-type and a decrease of leaf starch content, but no change in sucrose content
-
expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
expression of the His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), expression of untagged mutant H329A in Escherichia coli strain BL21(DE3)
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gene pgmA, sequence comparison, phylogenetic analysis and expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) in inclusion bodies
gene pgmG, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, overexpression of the His-tagged enzyme in Escherichia coli strain BL21(DE3), cloning in Escherichia coli strain DH5alpha
genes pgm1, pgm2, and pgm3, cloning in Escherichia coli strain DH5alpha, expression of His-tagged enzymes in Escherichia coli strain BL21 (DE3)
-
overexpression in Escherichia coli
overexpression of histidine-tagged fusion protein in Escherichia coli