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5.3.4.1: protein disulfide-isomerase

This is an abbreviated version!
For detailed information about protein disulfide-isomerase, go to the full flat file.

Word Map on EC 5.3.4.1

Reaction

catalyses the rearrangement of -S-S- bonds in proteins =

Synonyms

5'-MD, 58 kDa glucose regulated protein, 58 kDa microsomal protein, AGR2, anterior gradient homolog 2, BPA-binding protein, CaBP1, CaBP2, Cellular thyroid hormone binding protein, cotyledon-specific chloroplast biogenesis factor CYO1, CYO1, DbsG, disulfide bond isomerase, disulfide bond-forming enzyme, Disulfide interchange enzyme, disulfide isomerase, Disulfide isomerase ER-60, disulfide-bond isomerase, dithiol-disulfide isomerase, Dsb, DsbA, DsbB, DsbC, DsbD, DsbG, ECaSt/PDI, endoplasmic reticulum protein EUG1, Eps1p, ER protein 57, ER58, ER60, ERcalcistorin/protein-disulfide isomerase, ERdj5, Ero1, Erp, ERP-57, ERp-72 homolog, ERp18, ERp27, ERp28, ERp44, Erp46, ERp5, ERp57, ERP59, ERP60, ERp72, Eug1p, fibronectin, gPDI-1, gPDI-2, gPDI-3, HIP-70, HlPDI-1, HlPDI-2, HlPDI-3, Iodothyronine 5'-monodeiodinase, More, Mpd1p, Mpd2p, multifunctional protein disulfide isomerase, ncgl2478, P5, P55, P58, pancreas-specific protein disulfide isomerase, PDI, PDI A4, PDI I, PDI II, pdi-15, PDI-1a, pdi-40, pdi-47, pdi-52, PDI-A, PDI-M, PDI-P5, PDI-related protein, PDI1, PDI11, PDI2, PDI7, PDI8, PDIA1, PDIA2, PDIA3, PDIA4, PDIA6, PDIL-1, PDIL-2, PDIL1-1, PDIL1;1, PDIL1Aalpha, PDIL1B, PDIL2, PDIL2-3, PDIL3A, PDIL4D, PDIL5A, PDILT, PDIp, PDIr, protein disulfide isomerase, protein disulfide isomerase 1, protein disulfide isomerase 2, protein disulfide isomerase 3, protein disulfide isomerase A1, protein disulfide isomerase A3, protein disulfide isomerase A5, protein disulfide isomerase A6, protein disulfide isomerase associated 3, Protein disulfide isomerase P5, protein disulfide isomerase-1, protein disulfide isomerase-11, protein disulfide isomerase-2, protein disulfide isomerase-3, protein disulfide isomerase-8, protein disulfide isomerase-like protein of the testis, protein disulfide isomerase-P5, protein disulfide isomerase-related chaperone Wind, Protein disulfide isomerase-related protein, protein disulfide oxidoreductase, protein disulfide reductase/isomerase, protein disulfide-isomerase A4, Protein disulphide isomerase, Protein ERp-72, protein-disulfide isomerase, R-cognin, RB60, Rearrangease, Reduced ribonuclease reactivating enzyme, Retina cognin, S-S rearrangase, SSO0192, SsPDO, thiol-disulfide oxidoreductase, thiol-protein oxidoreductase, thioredoxin domain-containing protein 5, Thyroid hormone-binding protein, Thyroxine deiodinase, TXNDC5, yPDI

ECTree

     5 Isomerases
         5.3 Intramolecular oxidoreductases
             5.3.4 Transposing S-S bonds
                5.3.4.1 protein disulfide-isomerase

Cloned

Cloned on EC 5.3.4.1 - protein disulfide-isomerase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
baculovirus expression system
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cDNA cloning from library, DNA and amino acid sequence determination and analysis, genetic structure comparison and phylogenetic tree
cloning from RNA by 3' race RT-PCR, DNA and amino acid sequence determination and analysis, sequence comparison, expression as GST-tagged or His-tagged enzyme in Escherichia coli strain BL21(DE3)
cloning of cDNA
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cloning of the a and a' domain, expression in Escherichia coli
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coexpression of wild-type or mutant AGR2 with cysteine-rich regions of MUC2
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DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, expression of the enzyme fused to the human tissue plasminogen activator signal sequence in Escherichia coli strain DH10B
DNA and amino acid sequence determination and anaylsis, phylogenetic tree, expression in Escherichia coli as a soluble cytoplasmic protein
DNA and amino acid sequence determination, expression of His-tagged enzyme in Escherichia coli strain DH5alpha
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DNA sequence determination of PDI-1 and PDI-2, which show low overall identity, genomic organization and expression analysis in Trypanosoma brucei, expression of both enzyme forms as GST-tagged proteins in Escherichia coli
DNA sequence determination of testis-specific PDILT, expression in HeLa cells endoplasmic reticulum as Myc-tagged protein
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DNA sequence determination, expression of GFP-tagged wild-type enzyme and mutants Y53S and K84D in COS-7 cells, expression of C-terminally or N-terminally His6-tagged wild-type and mutant Wind in Escherichia coli strain XL 1-Blue and in Vero cells, the affinity for substrate Pipe is higher with the N-terminally His-tagged enzyme compared to the C-terminally tagged one
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enzyme form DsbD
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expressed in Escherichia coli
expressed in Escherichia coli and Pichia pastoris strain GS115
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expressed in Escherichia coli BL21 (DE3) pLysS cells
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expressed in Escherichia coli BL21 cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3)pLysS cells
expressed in Escherichia coli disulfide oxidase-deficient cells
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expressed in Escherichia coli Origami B (DE3) cells
expressed in Escherichia coli XL1-Blue MRF' cells
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expressed in H-1299 lung cancer cells
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expressed in Nicotiana benthamiana leaves
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expression Escherichia coli
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expression in Bacillus brevis
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expression in Bombyx mori pupae
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expression in COS-7 cell
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expression in COS-7 cells
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expression in Escherichia coli
expression in Escherichia coli and Sf9 insect cells
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expression of b and b' domains and the bb' fragments of ERp57 and ERp72 in Escherichia coli
expression of enzymatically nonfunctional PDI can restore Chlamydia sp. attachment but not entry into mutant CHO6 cells, while expression of functional PDI restores the complete Chlamydia infection, overview
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expression of enzyme form PDIp in Escherichia coli
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expression of GFP- or ORF-tagged RB60, wild-type and deletion mutants, in vitro protein synthesis using the T3 TNT-coupled reticulocyte lysate system
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expression of GST-fusion full-length ERp57 lacking the signal sequence, A24-L505, and the bb', P134-G376, and b', F241-G376, fragments in Escherichia coli strain BL21(DE3)
expression of GST-PDI fusion protein in Escherichia coli
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expression of GST-tagged bb' fragment, residues P135-S357, and of b' fragment, residues L236-S357, in Escherichia coli strain Bl21(DE3)
expression of His-tagged enzyme in Escherichia coli
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expression of His-tagged enzyme in Escherichia coli strain AD494(DE3)
expression of His-tagged PDI in Escherichia coli periplasmic space
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expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
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expression of His-tagged wild-type and mutant PDIs in Escherichia coli strain M15
expression of His-tagged wild-type PDI and mutant abb'a' lacking the C-terminal domain c in Escherichia coli strain BL21(DE3)
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expression of His-tagged wild-type PDI and mutant PDI defective in the a' domain, which bears the isomerase activity, in Escherichia coli
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expression of His6-tagged DsbC
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expression of His6-tagged isolated dimerization domains of DsbC and DsbG in strain BL21 (DE3)
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expression of myc-tagged mutant PDI in HeLa cells
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expression of PDI b domain in Escherichia coli
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expression of protein disulfide isomerase a domain in Escherichia coli
expression of residues 23-522 of PDI1
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expression of the domains and domain combinations in Escherichia coli
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expression of wild-type and mutant ERP-57 in COS-7 cells, co-expression with wild-type and mutant receptors, broken disulfide bond bridge mutant hGnRH receptors and calnexin, interaction analysis, overview
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expression of wild-type and mutant PDI in Escherichia coli strain BL21(DE3)
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expression Sf9 insect cells
expression using baculovirus system
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functional and mutational analysis of a conserved family of three PDIs in Caenorhabditis elegans
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functional complementation of an enzyme-deficient PDI-2-mutant strain of Caenorhabditis elegans, overview
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gene cyo1, isolation of CYO1 from the cyo1 mutant strain by T-DNA tagging, DNA and amino acid sequence determination and analysis, functional complementation by the wild-type cyo1 gene, cyo1 expression analysis, overview
gene HlPDI-1, DNA and amino acid sequence determination and anaylsis, phylogenetic tree, functional expression in Escherichia coli strain BL21 (DE3)
gene HlPDI-2, DNA and amino acid sequence determination and anaylsis, phylogenetic tree, functional expression in Escherichia coli strain BL21 (DE3)
gene HlPDI-3, DNA and amino acid sequence determination and anaylsis, phylogenetic tree, functional expression in Escherichia coli strain BL21 (DE3)
gene ncgl2478, quantitative real?time PCR analysis, sequence comparisons, recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene pdi, DNA and amino acid sequence determination and analysis, His-tagged PDI is co-expressed with the cyclotide precursor protein Oak1 in Escherichia coli strain BL21(DE3) using the pREP4 helper plasmid
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gene PDI, DNA and amino acid sequence determination and analysis, restriction mapping, expression in Escherichia coli strain MV1184
gene pdi, DNA and amino avid sequence determination and analysis, expression of PDI in Pichia pastoris strain overexpressing the Plasmodium falciparum disulfide-rich, transmission-blocking vaccine candidate protein Pfs25, isozymes A and B, the co-expression results in 3fold enhanced levels of the Pfs25 isozymes A and B as well as in enhaced PpPDI expression, overview
gene pdi, DNA and amino avid sequence determination and anaylsis, expression of PDI in Pichia pastoris strain overexpressing the Plasmodium falciparum disulfide-rich, transmission-blocking vaccine candidate protein Pfs25, isozymes A and B, the co-expression results in enhanced levels of the Pfs25 isozymes A and B as well as enhanced PfPDI expression, overview
AY919669
gene PDI2, DNA and amino acid sequence determination and analysis, cloning into the Escherichia coli-yeast shuttle vector pRS316 to generate the recombinant plasmid pYPDI2 in Escherichia coli strain strain MV1184, which is recombinantly introduced in Schizosaccharomyces pombe cells, that show up to 2.73fold increased PDI enzyme activity, with significantly higher activity in the stationary phase than in the exponential phase
gene TXNDC5, recombinant expression of Myc-tagged wild-type and mutant TXNDC5s in HEK-293T cells, pull-down assay with recombinant, co-expressed FLAG-tagged Srx
genes dsbA and dsbC, DNA sequence determination and analysis, expression of His-tagged DsbA and DsbC in Escherichia coli strain BL(DE3)
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genes PDIS-1 and PDIS-2, DNA and amino acid sequence determination and anaylsis, expression in Escherichia coli as soluble folded proteins
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GmPDIL-3a and GmPDIL-3b isozymes, cloning of mature GmPDIL-3a (Thr24-Leu520) and GmPDIL-3b (Ser27-Leu523), excluding the putative signal peptides, expression in Escherichia coli strain BL21(DE3)
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in addition to a dsbA-like gene, a dsnC gene, coding for a periplasmic protein with disulfide isomerase activity
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isozyme pdi-15, DNA and amino acid sequence determination and analysis, sequence comparison with other Leishmania species, overview
isozyme pdi-40, DNA and amino acid sequence determination and analysis, sequence comparison with other Leishmania species, overview
isozyme pdi-47, DNA and amino acid sequence determination and analysis, sequence comparison with other Leishmania species, overview
isozyme pdi-52, DNA and amino acid sequence determination and analysis, sequence comparison with other Leishmania species, overview
isozymes PDI-8, PDI-9, PDI-11, and PDI-14, localization on chromosome-8, chromosome-9, chromosome-11, and chromosome-14, respectively, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, expression of the enzyme fused to the human tissue plasminogen activator signal sequence in Escherichia coli, strain DH10B
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isozymes PDIA3 and PDI-P5 precursors, DNA and amino acid sequence determination and analysis, expression of His-tagged mature isozymes in Escherichia coli strain HMS174 (DE3)
overexpression in Bacillus brevis, secretion to the cell medium
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overexpression in Escherichia coli
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overexpression of DsbC in strain BL21(DE3)
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overexpression of endogenous PDI in clone Pfs25
overexpression of PDI in GH3 cells, that release growth hormone via the T3-receptor, leads to reduced T3-induced GH release in the cells, mechanism, overview
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overexpression of wild-type and mutant enzyme in Escherichia coli strain BL21(DE3)
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overexpression of wild-type isozymes A and B and of the chimeric PDI mutant in Pichia pastoris using the PFs25 clone, co-expression of Pichia pastoris PDI in Pfs25
AY919669
PDI cloning from CHO-K1 cells, expression as N-terminally HA-tagged enzyme in GP2-293 cells, complementation by expression of full-length PDI restores Chlamydia trachomatis binding and infectivity in the enzyme-deficient CHO-6 mutant cell line, as wellas to diphtheria toxin sensitivity, PDI might complement an endoplasmic reticulum transport function, or other indirect functions, resulting in the change of phenotype for chlamydial susceptibility, overview
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PDI, DNA sequence determination and analysis
PDI, expression of substrate-trapping mutants of PDI family members ERp46, ERp18, ERp57, ERp72, and P5 in HT-1080 cells, the mutant enzymes form mixed disulfides
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PDI, quantitative expression analysis, overexpression in CHO and in NS0/2N2 cells
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PDI, quantitative expression analysis, overexpression in CHO cells
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PDIA3, DNA and amino acid sequence determination and analysis, quantitative expression analysis, genetic structure and phylogenetic analysis
protein disulfide isomerase and the beta-subunit of prolyl 4-hydroxylase, EC 1.14.11.2, are products of the same gene
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recombinant constitutive overexpression in Mus musculus NSC-34 cells and neuroblastoma Neuro2a cells. PDI overexpression decreases mutant SOD1-induced cell death, overview
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recombinant expression of wild-type and mutant enzymes in Escherichia coli. Recombinant PDIp protects the Escherichia coli cells against heat shock and oxidative stress-induced cell death independently of its enzymatic activity
sequence comparison
the gene encoding ERp27 is located at 14,958,241–14,982,750 bp on chromosome 12 and has 7 exons, DNA and amino acid sequence determination and anaylsis, sequence comparison, expression of GFP-fusion ERp27 in COS-7 cell endoplasmic reticulum, expression of N-terminally His-tagged wild-type and mutant ERp57, and of PDI a, b, and b' domains, in Escherichia coli strain BL21(DE3)
transfection of EA.hy926 cells
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wild-type and/or mutant PDI overexpression in vascular smooth muscle cells and in HEK-293T cells
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