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5.3.3.2: isopentenyl-diphosphate DELTA-isomerase

This is an abbreviated version!
For detailed information about isopentenyl-diphosphate DELTA-isomerase, go to the full flat file.

Word Map on EC 5.3.3.2

Reaction

isopentenyl diphosphate
=
dimethylallyl diphosphate

Synonyms

fcd1, FNI, IDI, IDI-1, Idi-2, IDI1, IDP isomerase, IPI, IPI1, IPP isomerase, IPP-isomerase, IPP:DMAPP, IPPI, IPPI1, IPPI2, IPPISOM, Isomerase, isopentenylpyrophosphate DELTA-, Isopententenyl diphosphate:dimethylallyl diphosphate isomerase, isopentenyl diphosphate isomerase, isopentenyl diphosphate isomerase 1, isopentenyl diphosphate:dimethylallyl diphosphate isomerase, Isopentenyl pyrophosphate isomerase, Isopentenyl pyrophosphate isomerase:dimethylallyl pyrophosphate isomerase, isopentenyl-diphosphate delta-isomerase 1, Isopentenyldiphosphate DELTA-isomerase, Isopentenylpyrophosphate isomerase, Methylbutenylpyrophosphate isomerase, SlIPI, type 1 IPP isomerase, type 2 isopentenyl diphosphate isomerase, type 2 isopentenyl diphosphate: dimethylallyl diphosphate isomerase, type 2 isopentenyl diphosphate:dimethylallyl diphosphate isomerase, type 2 isopentenyl-diphosphate isomerase, type I isopentenyl diphosphate isomerase, type II isopentenyl diphosphate isomerase, type II isopentenyl diphosphate:dimethylallyl diphosphate isomerase, type-2 isopentenyl diphosphate isomerase, type-I isopentenyl pyrophosphate isomerase

ECTree

     5 Isomerases
         5.3 Intramolecular oxidoreductases
             5.3.3 Transposing C=C bonds
                5.3.3.2 isopentenyl-diphosphate DELTA-isomerase

Purification

Purification on EC 5.3.3.2 - isopentenyl-diphosphate DELTA-isomerase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 isoforms: I and II
Cinchona robusta
-
by affinity chromatography on Ni-NTA resin
Co2+ column chromatography, and gel filtration
-
cobalt column chromatography
-
DEAE Sepharose column chromatography and Resource Phe column chromatograpyh
-
His-Trap column chromatography
Ni-NTA agarose column chromatography
-
Ni-NTA agarose resin column chromatography
-
Ni-NTA column chromatography
-
nickel-nitrilotriacetic acid affinity chromatography
-
on a HisTrap column, for crystallisation, the enzyme is loaded on a HiLoad 16/60 Superdex 200 column
partial
-
partial. Dye-ligand and immobilized metal ion interaction chromatography are efficient techniques for the rapid batchwise fractionation, from crude plant extracts, of a series of enzymes of prenyl diphosphate metabolism
-
recombinant enzyme
recombinant protein, enzyme purified under aerobic conditions is inactive until the flavin cofactor is reduced by NADPH or dithionite or photochemically
-
TALON metal affinity resin column chromatography