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5.3.1.6: ribose-5-phosphate isomerase

This is an abbreviated version!
For detailed information about ribose-5-phosphate isomerase, go to the full flat file.

Word Map on EC 5.3.1.6

Reaction

D-ribose 5-phosphate
=
D-ribulose 5-phosphate

Synonyms

5-phosphate ketol-isomerase, 5-Phosphoribose isomerase, CTRPI, CTRpiB, D-Ribose 5-phosphate isomerase, D-ribose-5-phosphate isomerase, D-ribose-5-phosphate isomerase A, D-ribose-5-phosphate isomerase B, D-ribose-5-phosphate ketol-isomerase, D-xylose ketol-isomerase, EcRpiB, Isomerase, ribose phosphate, LINJ_28_2100, LMJF_28_1970, MJ1603, MtRpiB, Phosphopentoisomerase, Phosphopentose isomerase, Phosphoriboisomerase, ribose 5-phosphate isomerase A, Ribose phosphate isomerase, Ribose-5-P isomerase, ribose-5-phosphate isomerase, ribose-5-phosphate isomerase B, ribose-5-phosphate isomerase type B, Ribosephosphate isomerase A, Ribosephosphate isomerase B, RPI, RPI2 protein, RpiA, RpiB, SMU1234, SMU2142, Tc00.1047053509199.24, TM1080, type B ribose 5-phosphate isomerase, type B ribose-5-phosphate isomerase, type B Rpi

ECTree

     5 Isomerases
         5.3 Intramolecular oxidoreductases
             5.3.1 Interconverting aldoses and ketoses, and related compounds
                5.3.1.6 ribose-5-phosphate isomerase

Purification

Purification on EC 5.3.1.6 - ribose-5-phosphate isomerase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
3 forms: I, I, III
-
affinity chromatographic method
-
heat precipitation
-
HisTrap HP column chromatography
HiTrap Q anion exchange column chromatography and Sephacryl S-300 gel filtration
-
Ni-affinity column chromatography
-
Ni-NTA column chromatography and Superdex 75 gel filtration
Ni2+-chelating column chromatography and Superdex S75 gel filtration
ox muscle, calf spleen and liver, partial
-
recombinant enzyme
-
recombinant His-tagged RpiA from Escherichia coli strain BL21(DE3) by nickel affinity chromatography. The His-tag is cleaved off and removed, folowed by anion exchange chromatography and gel filtration
recombinant MJ1603 from Escherichia coli strain Rosetta (DE3) by anion exchange and hydroxyapatite chromatography, and gel filtration
recombinant RpiB from Escherichia coli strain ER2566 by His affinity chromatography
-
Superdex 75 gel filtration