Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

5.3.1.5: xylose isomerase

This is an abbreviated version!
For detailed information about xylose isomerase, go to the full flat file.

Word Map on EC 5.3.1.5

Reaction

alpha-D-xylopyranose
=
alpha-D-xylufuranose

Synonyms

D-XI, D-xylose aldose-ketose-isomerase, D-xylose isomerase, D-Xylose ketoisomerase, D-xylose ketol isomerase, D-xylose ketol-isomerase, D-xylose: ketol-isomerase, D-xylulose keto-isomerase, glucose isomerase, glucose/xylose isomerase, GXI, Isomerase, xylose, Maxazyme, Optisweet, SDXyI, Spezyme, Sweetase, Sweetzyme, Sweetzyme Q, Swetase, T80 xylose isomerase, TcaXI, TNXI, TthXI, XI, XYLA, XylC, xylose (glucose) isomerase, xylose isomerase

ECTree

     5 Isomerases
         5.3 Intramolecular oxidoreductases
             5.3.1 Interconverting aldoses and ketoses, and related compounds
                5.3.1.5 xylose isomerase

Purification

Purification on EC 5.3.1.5 - xylose isomerase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, Dowex-1 anion exchange column chromatography, and Sephadex G-100 gel filtration
ammonium sulfate precipitation, Dowex-1 column chromatography, and Sephadex G-100 gel filtration
-
ammonium sulfate precipitation, Ni-NTA column chromatography, and Superdex 200 gel filtration
from a stock solution of a food grade product, 2 counterdiffusion dialysis devices: diffusion-controlled apparatus for microgravity and counterdiffusion cell, growth of crystals approx. 3 months
gel filtration over Sephacryl S200 - dialysis against 0.05 g/l MgSO4, purity control verified with capillary electrophoresis
-
gel filtration with Sephacryl S-200 high resolution media, running buffer composed of 0.05 M sodium phosphate pH 7.7, 0.1 M sodium chloride and 0.02% azide - final purity of xylose isomerase: 96%
-
heat treatment and DEAE-Sepharose column chromatography
heat treatment, DEAE-Sepharose column chromatography and Q-Sepharose column chromatography
-
HisPrep FF 16/10 column chromatography
-
HisTrap crude nickel affinity column chromatography
-
HiTrap Ni-chelating column chromatography
immunoaffinity purification
-
Ni-NTA column chromatography
one-step purification by high-performance immobilized copper-affinity chromatography
-
PAGE and SDS-PAGE, identical electrophoretic mobility of intact and denatured enzyme compared with xylose isomerase from Arthrobacter nicotianae and Erwinia carotovora subsp. atroseptica
-
PAGE and SDS-PAGE, identical electrophoretic mobility of intact and denatured enzyme compared with xylose isomerase from Escherichia coli and Erwinia carotovora subsp. atroseptica
-
PAGE and SDS-PAGE, identical electrophoretic mobility of intact and denatured enzyme compared with xylose isomerase from from Arthrobacter nicotianae and Escherichia coli
-
purified enzyme is insoluble and inactive
-
purified enzyme is soluble
-
recombinant enzyme from Escherichia coli strain BL21(DE3)
-
recombinant enzyme from Escherichia coli strain BL21(DE3) by one-step affinity chromatography to over 90% purity
-
recombinant enzyme, by heat precipitation and cation exchange, proteins were 95% pure when estimated in SDS-PAGE
Talon metal affinity column chromatography and Superdex 75 gel filtration
to homogeneity
-