5.3.1.5: xylose isomerase
This is an abbreviated version!
For detailed information about xylose isomerase, go to the full flat file.
Word Map on EC 5.3.1.5
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5.3.1.5
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isomerization
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biomass
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d-glucose
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xylitol
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xylulokinase
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pentose
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lignocellulosic
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isomerases
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syrup
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corn
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gi
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d-fructose
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rubiginosus
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bioethanol
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piromyces
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arthrobacter
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l-arabinose
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synthesis
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actinoplanes
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high-fructose
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xylose-fermenting
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transaldolase
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saccharification
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thermoanaerobacter
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co-fermentation
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hemicellulosic
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thermoanaerobacterium
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neapolitana
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stipitis
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energy production
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orpinomyces
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thermosulfurogenes
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xylose-utilizing
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diauxic
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food industry
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nutrition
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biotechnology
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degradation
- 5.3.1.5
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isomerization
- biomass
- d-glucose
- xylitol
- xylulokinase
- pentose
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lignocellulosic
- isomerases
- syrup
- corn
- gi
- d-fructose
- rubiginosus
-
bioethanol
- piromyces
- arthrobacter
- l-arabinose
- synthesis
- actinoplanes
-
high-fructose
-
xylose-fermenting
- transaldolase
-
saccharification
- thermoanaerobacter
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co-fermentation
-
hemicellulosic
- thermoanaerobacterium
- neapolitana
- stipitis
- energy production
- orpinomyces
- thermosulfurogenes
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xylose-utilizing
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diauxic
- food industry
- nutrition
- biotechnology
- degradation
Reaction
Synonyms
D-XI, D-xylose aldose-ketose-isomerase, D-xylose isomerase, D-Xylose ketoisomerase, D-xylose ketol isomerase, D-xylose ketol-isomerase, D-xylose: ketol-isomerase, D-xylulose keto-isomerase, glucose isomerase, glucose/xylose isomerase, GXI, Isomerase, xylose, Maxazyme, Optisweet, SDXyI, Spezyme, Sweetase, Sweetzyme, Sweetzyme Q, Swetase, T80 xylose isomerase, TcaXI, TNXI, TthXI, XI, XYLA, XylC, xylose (glucose) isomerase, xylose isomerase
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Purification
Purification on EC 5.3.1.5 - xylose isomerase
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ammonium sulfate precipitation, Dowex-1 anion exchange column chromatography, and Sephadex G-100 gel filtration
ammonium sulfate precipitation, Dowex-1 column chromatography, and Sephadex G-100 gel filtration
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ammonium sulfate precipitation, Ni-NTA column chromatography, and Superdex 200 gel filtration
from a stock solution of a food grade product, 2 counterdiffusion dialysis devices: diffusion-controlled apparatus for microgravity and counterdiffusion cell, growth of crystals approx. 3 months
gel filtration over Sephacryl S200 - dialysis against 0.05 g/l MgSO4, purity control verified with capillary electrophoresis
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gel filtration with Sephacryl S-200 high resolution media, running buffer composed of 0.05 M sodium phosphate pH 7.7, 0.1 M sodium chloride and 0.02% azide - final purity of xylose isomerase: 96%
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heat treatment and DEAE-Sepharose column chromatography
heat treatment, DEAE-Sepharose column chromatography and Q-Sepharose column chromatography
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one-step purification by high-performance immobilized copper-affinity chromatography
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PAGE and SDS-PAGE, identical electrophoretic mobility of intact and denatured enzyme compared with xylose isomerase from Arthrobacter nicotianae and Erwinia carotovora subsp. atroseptica
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PAGE and SDS-PAGE, identical electrophoretic mobility of intact and denatured enzyme compared with xylose isomerase from Escherichia coli and Erwinia carotovora subsp. atroseptica
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PAGE and SDS-PAGE, identical electrophoretic mobility of intact and denatured enzyme compared with xylose isomerase from from Arthrobacter nicotianae and Escherichia coli
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recombinant enzyme from Escherichia coli strain BL21(DE3) by one-step affinity chromatography to over 90% purity
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recombinant enzyme, by heat precipitation and cation exchange, proteins were 95% pure when estimated in SDS-PAGE
Talon metal affinity column chromatography and Superdex 75 gel filtration
ammonium sulfate precipitation, Dowex-1 anion exchange column chromatography, and Sephadex G-100 gel filtration
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ammonium sulfate precipitation, Dowex-1 anion exchange column chromatography, and Sephadex G-100 gel filtration
Opuntia vulgaris
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