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5.3.1.5: xylose isomerase

This is an abbreviated version!
For detailed information about xylose isomerase, go to the full flat file.

Word Map on EC 5.3.1.5

Reaction

alpha-D-xylopyranose
=
alpha-D-xylufuranose

Synonyms

D-XI, D-xylose aldose-ketose-isomerase, D-xylose isomerase, D-Xylose ketoisomerase, D-xylose ketol isomerase, D-xylose ketol-isomerase, D-xylose: ketol-isomerase, D-xylulose keto-isomerase, glucose isomerase, glucose/xylose isomerase, GXI, Isomerase, xylose, Maxazyme, Optisweet, SDXyI, Spezyme, Sweetase, Sweetzyme, Sweetzyme Q, Swetase, T80 xylose isomerase, TcaXI, TNXI, TthXI, XI, XYLA, XylC, xylose (glucose) isomerase, xylose isomerase

ECTree

     5 Isomerases
         5.3 Intramolecular oxidoreductases
             5.3.1 Interconverting aldoses and ketoses, and related compounds
                5.3.1.5 xylose isomerase

Cloned

Cloned on EC 5.3.1.5 - xylose isomerase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning and functional expression of enzymes from Escherichia coli in Pseudomonas putida
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co-overexpression of genes encoding xylose isomerase (EcxylA, Escherichia coli) and xylulokinase (XYL3, Hansenula polymorpha), expression of both genes is driven by Hansenula polymorpha GAP-DH promoter
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Corynebacterium glutamicum is transformed with: xylose isomerase (xylA, Escherichia coli) and xylulokinase (xylB, Escherichia coli)-two recombinant strains of Corynebacterium glutamicum are obtained: CRX1 (xylA) and CRX2 (xylA/xylB)
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expressed in Escherichia coli BL21 (DE3) cells
expressed in Escherichia coli BL21 Star (DE3) cells and in Lactobacillus plantarum
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expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli DH10B cells
expressed in Escherichia coli Rosetta2 (DE3)pLysS cells
expressed in Saccharomyces cerevisiae
expressed in Saccharomyces cerevisiae strain BY4741-S1
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expression in Escherichia coli
expression in Escherichia coli (DH5-alpha) and Saccharomyces cerevisiae
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expression in Escherichia coli BL21(DE3)
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expression in Escherichia coli HB101
expression in Escherichia coli JM105
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expression in Escherichia coli or Bacillus subtilis
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expression in Escherichia coli, sequence homology with enzymes from other sources
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expression in Hansenula polymorpha
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expression in Pseudomonas putida S12
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expression in Saccharomyces cerevisiae
expression in the yeast Hansenula polymorpha
expression of mutant enzymes in Escherichia coli strain BL21 (DE3)
gene XYL1, subcloning in Escherichia coli strain MC1061, functional expression in Corynebacterium glutamicum, ATCC13032. Biotransformation of xylose into xylitol appears to be influenced by xylose transport, which is in turn affected by the glucose concentration in the reaction medium
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gene xylA, expression in Saccharomyces cerevisiae
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gene xylA, expression of the polyhydroxybutyrate producing enzyme in an enzyme-deficient knockout strain of Burkholderia sacchari restoring the ability of the cells to produce polyhydroxybutyrate and increasing cell growth, overview
overexpression in Escherichia coli strain BL21(DE3) from vector pRAC
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phylogenetic analysis and enzyme sequence comparisons, functional expression of codon-optimized enzyme in Saccharomyces cerevisiae confers on the yeast cells the ability to metabolize D-xylose and to use it as the sole carbon and energy source. The recombinant enzyme shows reduced sensitivity to inhibition by xylitol
phylogenetic analysis, enzyme expression in Escherichia coli strain BL21(DE3)
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Saccharomyces cerevisiae is transformed with a gene encoding xylose isomerase (Thermus thermophilus)
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Saccharomyces cerevisiae is transformed with a gene encoding xylose isomerase originating from Piromyces sp.
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the production of the cloned gene in Escherichia coli and Bacillus brevis are compared expression in Escherichia coli and Bacillus brevis. Bacillus brevis is able to produce the isomerase efficiently (more than 1 g/l)
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transformants bearing the fused lac-xylA or tac-xylA gene, cloned into various high copy-number plasmids
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wild-type and mutant enzymes, expression in Escherichia coli
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wild-type and mutant enzymes, overexpression in Escherichia coli
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xylose isomerase gene (Piromyces sp.) is overexpressed in Saccharomyces cerevisiae strain TMB3044 resulting in strain TMB 3066
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Zymobacter palmae is transformed with: xylose isomerase (xylA, Escherichia coli), xylulokinase (xylB, Escherichia coli), transaldolase (tal, Escherichia coli), transketolase (tktA, Escherichia coli). Different promotors from Zymomonas mobilis ATCC 29191 are used: gap-dh (Pgap), enolase (Peno)
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