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5.1.3.8: N-acylglucosamine 2-epimerase

This is an abbreviated version!
For detailed information about N-acylglucosamine 2-epimerase, go to the full flat file.

Word Map on EC 5.1.3.8

Reaction

N-acyl-D-glucosamine
=
N-acyl-D-mannosamine

Synonyms

Acylglucosamine 2-epimerase, AGE, AGE2, anAGE, AvaAGE, bage, bGlcNAc 2-epimerase, Epimerase, acylglucosamine 2-, GlcNAc 2-epimerase, GlcNAc-2-epimerase, N-acetyl-D-glucosamine 2-epimerase, N-acetyl-D-glucosamine-2-epimerase, N-Acetylglucosamine 2-epimerase, N-acyl-D-glucosamine 2-epimerase, N-acylglucosamine 2-epimerase, PhGn2E, renin binding protein, Renin-binding protein, RNBP

ECTree

     5 Isomerases
         5.1 Racemases and epimerases
             5.1.3 Acting on carbohydrates and derivatives
                5.1.3.8 N-acylglucosamine 2-epimerase

Purification

Purification on EC 5.1.3.8 - N-acylglucosamine 2-epimerase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by FP-based affinity chromatography
of the recombinant protein by immobilized nickel-ion chromatography
of the recombinant proteins, wild type and mutants by immobilized nickel-ion chromatography
recombinant enzyme
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, copurification with recombinant Escherichia coli N-acetyl-D-neuraminic acid aldolase
recombinant His-tagged enzyme from Escherichia coli strain Rosetta (DE3)pLys by ultrafiltration and nickel affinityy chromatography
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3)
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain NovaBlue (DE3) by nickel affinity chromatography and dialysis