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5.1.3.8: N-acylglucosamine 2-epimerase

This is an abbreviated version!
For detailed information about N-acylglucosamine 2-epimerase, go to the full flat file.

Word Map on EC 5.1.3.8

Reaction

N-acyl-D-glucosamine
=
N-acyl-D-mannosamine

Synonyms

Acylglucosamine 2-epimerase, AGE, AGE2, anAGE, AvaAGE, bage, bGlcNAc 2-epimerase, Epimerase, acylglucosamine 2-, GlcNAc 2-epimerase, GlcNAc-2-epimerase, N-acetyl-D-glucosamine 2-epimerase, N-acetyl-D-glucosamine-2-epimerase, N-Acetylglucosamine 2-epimerase, N-acyl-D-glucosamine 2-epimerase, N-acylglucosamine 2-epimerase, PhGn2E, renin binding protein, Renin-binding protein, RNBP

ECTree

     5 Isomerases
         5.1 Racemases and epimerases
             5.1.3 Acting on carbohydrates and derivatives
                5.1.3.8 N-acylglucosamine 2-epimerase

Cloned

Cloned on EC 5.1.3.8 - N-acylglucosamine 2-epimerase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 recombinant Escherichia coli strains capable of expressing N-acetyl-D-glucosamine 2-epimerase and N-acetyl-D-neuraminic acid aldolase are constructed based on a highly efficient temperature-responsive expression system which is safe compared to chemical-induced systems and coupled in N-acetyl-D-neuraminic acid production
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construction of a series of chimeric enzymes successively replacing the three domains of the human enzyme - N-terminal, middle, and C-terminal - with the corresponding domains of the rat enzyme. Chimeras are expressed in Escherichia coli JM109 under the control of the Taq promoter
construction of several C-terminal deletion and multi-cysteine/serine mutants and expression in Escherichia coli
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expressed in Escherichia coli JM109 under the transcriptional control of taq promoter
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expression in Escherichia coli
expression in Jurkat cells
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expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) in inclusion bodies, subcloning in Escherichia coli strain DH5alpha
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expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
expression of wild-type enzyme and mutant enzymes C41S, C66S, C104S, C125S, C210S, C239S, C302S, C380S, C386S and C390S in Escherichia coli
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gene age, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) at 37°C with coexpression of chaperones GroEL/GroES, 264fold increased AGE activity and maximum yield of 22.5 mg isolated AvaAGE per liter shake flask culture, compared to the starting conditions, method optimization, enhancement of soluble expression via chaperones, temperature dependence of soluble expression, and enhancement of soluble expression via fusion tags, overview
gene BACOVA_01816, expression of His-tagged enzyme in Escherichia coli strain Rosetta (DE3)pLys
gene bage, recombinant expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3), coexpression with N-acetyl-D-neuraminic acid aldolase gene nanA from Escherichia coli
recombinant enzyme expression in Escherichia coli, coexpression with N-acetylneuraminic acid lyase from Corynebacterium glutamicum ATCC 13032 (CgNal, EC 4.1.3.3)
recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain NovaBlue (DE3)