5.1.3.5: UDP-arabinose 4-epimerase

This is an abbreviated version!
For detailed information about UDP-arabinose 4-epimerase, go to the full flat file.

Word Map on EC 5.1.3.5

Reaction

UDP-L-arabinose
=
UDP-D-xylose

Synonyms

Epimerase, uridine diphosphoarabinose, Uridine diphosphoarabinose epimerase, UDP arabinose epimerase, Uridine 5'-diphosphate-D-xylose 4-epimerase, UDP-D-xylose 4-epimerase, UDP-arabinose 4-epimerase, UDP xylose-4-epimerase 1, UDP-xylose 4-epimerase, UDP-Xyl 4-epimerase, PsUGE1, AtUGE1, AtUGE2, AtUGE3, AtUGE4, AtUGE5, Uxe, UDP-alpha-D-xylose 4-epimerase, UXE3, UXE1, UXE2

ECTree

     5 Isomerases
         5.1 Racemases and epimerases
             5.1.3 Acting on carbohydrates and derivatives
                5.1.3.5 UDP-arabinose 4-epimerase

Cloned

Cloned on EC 5.1.3.5 - UDP-arabinose 4-epimerase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
expression in Agrobacterium tumefaciens
gene UXE1, transcriptome-guided search by transcriptome analysis of Ornithogalum caudatum unigenes, cloning, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of soluble His-tagged N-terminally truncated enzyme in Escherichia coli strain BL21(DE3) by coexpression with chaperones from plasmid pET28atOcUXE1 and chaperone plasmid pGro7, and pET28atOcUXE2 and another chaperone plasmid pKJE7. The recombinant expression in absence of chaperones results in misfolded enzyme in inclusion bodies, expression of full-length His-tagged enzyme is unsuccessful. The truncated recombinant enzyme is catalytically inactive
gene UXE2, transcriptome-guided search by transcriptome analysis of Ornithogalum caudatum unigenes, cloning, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of soluble His-tagged N-terminally truncated enzyme in Escherichia coli strain BL21(DE3) by coexpression with chaperones from plasmid pET28atOcUXE1 and chaperone plasmid pGro7, and pET28atOcUXE2 and another chaperone plasmid pKJE7. The recombinant expression in absence of chaperones results in misfolded enzyme in inclusion bodies, expression of full-length His-tagged enzyme is unsuccessful. The truncated recombinant enzyme is catalytically inactive
isoform UXE1 is expressed in Pichia pastoris
recombinant protein AtUGE1-5 is expressed in Escherichia coli with C-terminal His-tag
recombinant protein is expressed in Escherichia coli
recombinant protein is expressed in Escherichia coli with C-terminal His-tag