5.1.3.20: ADP-glyceromanno-heptose 6-epimerase

This is an abbreviated version!
For detailed information about ADP-glyceromanno-heptose 6-epimerase, go to the full flat file.

Word Map on EC 5.1.3.20

Reaction

ADP-D-glycero-D-manno-heptose
=
ADP-L-glycero-D-manno-heptose

Synonyms

ADP-L-glycero-D-mannoheptose 6-epimerase, Epimerase, adenosine diphosphoglyceromannoheptose 6-, Adenosine diphosphoglyceromannoheptose 6-epimerase, ADP-L-glycero-D-manno-heptose-epimerase, Aquifex aelicis gene rfaD, GenBank AE000684-derived protein GI 2983012, Epimerase, adenosine diphosphoglyceromannoheptose 6- (Neisseria gonorrhoeae gene lis-6), ADP-L-glycero-D-mannoheptose 6-epimerase (Neisseria gonorrhoeae gene gme), Epimerase, adenosine diphosphoglyceromannoheptose 6- (Escherichia coli clone pCG50 reduced), ADP-L-glycero-D-mannoheptose-6-epimerase (Escherichia coli strain K-12 substrain MG1655 clone EC19-98 gene rfaD), Genbank AE000440-derived protein GI 1790049, ADP-L-glycero-D-manno-heptose-6-epimerase, ADP-L-glycero-D-mannoheptose-6-epimerase, ADP-glyceromanno-heptose 6-epimerase, AGME, RfaD, hldD, ADP-L,D-hep 6-epimerase, ADP-L-glycero-D-manno-heptose 6-epimerase, ADP-glycero-manno-heptose-6-epimerase, GmhD, HP0859

ECTree

     5 Isomerases
         5.1 Racemases and epimerases
             5.1.3 Acting on carbohydrates and derivatives
                5.1.3.20 ADP-glyceromanno-heptose 6-epimerase

Purification

Purification on EC 5.1.3.20 - ADP-glyceromanno-heptose 6-epimerase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
metal affinity column chromatography, hydrophobic affinity column chromatography, and gel filtration
recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gell filtration
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography