5.1.3.17: heparosan-N-sulfate-glucuronate 5-epimerase
This is an abbreviated version!
For detailed information about heparosan-N-sulfate-glucuronate 5-epimerase, go to the full flat file.
Word Map on EC 5.1.3.17
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5.1.3.17
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l-iduronic
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d-glucuronic
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epimerization
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proteoglycans
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idoa
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glycosaminoglycans
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glca
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n-sulfated
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2-o-sulfotransferase
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hexuronic
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hspgs
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2-o-sulfation
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mastocytoma
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mannuronan
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lindahl
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o-desulfated
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medicine
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diagnostics
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analysis
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synthesis
- 5.1.3.17
-
l-iduronic
-
d-glucuronic
-
epimerization
- proteoglycans
-
idoa
- glycosaminoglycans
-
glca
-
n-sulfated
-
2-o-sulfotransferase
-
hexuronic
-
hspgs
-
2-o-sulfation
- mastocytoma
-
mannuronan
-
lindahl
-
o-desulfated
- medicine
- diagnostics
- analysis
- synthesis
Reaction
Synonyms
C5 uronosyl epimerase, C5-epi, C5-epimerase, D-Glucuronyl C-5 epimerase, D-glucuronyl C5-epimerase, Epimerase, polyglucuronate, Glce, GlceA, GlceB, glucuronosyl C-5 epimerase, glucuronyl C5-epimerase, heparan sulfate C-5 epimerase, Heparan sulfate C5-epimerase, Heparosan N-sulfate D-glucuronosyl 5-epimerase, heparosan-glucuronate 5-epimerase, Heparosan-N-sulfate-D-glucuronosyl-5-epimerase, HG-5epi, HNSG-5epi, HS C5-epimerase, HS glucuronyl C5-epimerase, Hsepi, RED-C5-epimerase
ECTree
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Crystallization
Crystallization on EC 5.1.3.17 - heparosan-N-sulfate-glucuronate 5-epimerase
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purified enzyme in apo-form and in complex with heparin hexasaccharide, both in a stable dimer conformation, hanging drop vapour diffusion method, mixing of 0.001 ml of 15-20 mg/ml protein in in 20 mM Tris, pH 8.0, 200 mM ammonium acetate, 1 mM dithiothreitol, and 1 mM EDTA, with 0.001 ml of 16% w/v PEG 3350, 0.1 M sodium citrate tribasic dihydrate, pH 5.6, and 2% v/v Tacsimate, pH 5.0 for the unlabeled enzyme and 16% w/v PEG 3350, 0.06 M citric acid, and 0.04 M Bis-Tris propane, pH 4.1, for the SeMet-labeled enzyme, a concentration of 5.0 mg/ml is used for the enzyme complex with heparin oligosaccharide at a molar ratio of 1:5, 20°C, 3 days, X-ray diffraction structure determination and analysis at 1.9-2.2 A resolution