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5.1.3.1: ribulose-phosphate 3-epimerase

This is an abbreviated version!
For detailed information about ribulose-phosphate 3-epimerase, go to the full flat file.

Word Map on EC 5.1.3.1

Reaction

D-ribulose 5-phosphate
=
D-xylulose 5-phosphate

Synonyms

cyt-RPEase, D-ribulose 5-phosphate 3-epimerase, D-Ribulose phosphate-3-epimerase, D-Ribulose-5-P 3-epimerase, D-ribulose-5-phosphate 3-epimerase, D-Ribulose-5-phosphate epimerase, D-ribulose-5-phosphate-3-epimerase, D-Xylulose-5-phosphate 3-epimerase, Epimerase, ribulose phosphate 3-, Pentose-5-phosphate 3-epimerase, Phosphoketopentose 3-epimerase, Phosphoketopentose epimerase, Phosphoribulose epimerase, PPE, R5P3E, Ribulose 5-phosphate 3-epimerase, Ribulose phosphate 3-epimerase, ribulose-5-phosphate 3-epimerase, RPE, rpe1, rpe1-MGA3, rpe1-PB1, rpe2, rpe2-MGA3, RPEase, rpeC, rpeP, Ru5P epimerase, Ru5Pepimerase, slr1622, Xylulose phosphate 3-epimerase, xylulose-5-P 3-epimerase

ECTree

     5 Isomerases
         5.1 Racemases and epimerases
             5.1.3 Acting on carbohydrates and derivatives
                5.1.3.1 ribulose-phosphate 3-epimerase

Engineering

Engineering on EC 5.1.3.1 - ribulose-phosphate 3-epimerase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D175A
inactive
D37A
the mutation almost abolishes the enzymatic activity
H35A
the mutation almost abolishes the enzymatic activity
H70A
completely insoluble
L12A
the mutation results in more than 50% decrease in the activity
M141A
the mutation results in about 30% decrease in the activity
M39A
the mutation results in about 10% decrease in the activity
M72A
the mutation results in an almost 50% decrease in the activity
S10A
the mutation almost abolishes the enzymatic activity
D186A
KM-value for D-ribulose 5-phosphate is increased 10fold, turnover-number is decreased to less than 0.1% of the wild-type value
D186E
KM-value for D-ribulose 5-phosphate is unaltered, turnover-number is decreased to less than 0.1% of the wild-type value
D186N
KM-value for D-ribulose 5-phosphate is unaltered, turnover-number is decreased to less than 0.1% of the wild-type value
D176A
no detectable activity, substrate= 10 mM D-ribulose 5-phosphate
D36A
no detectable activity, substrate= 10 mM D-ribulose 5-phosphate
H34A
mutant discloses decreased affinity for Zn2+, kcat = 200/sec, substrate = 10 mM D-ribulose 5-phosphate, +10 micromol/l ZnCl2
H67A
mutant discloses decreased affinity for Zn2+, kcat = 270/sec, substrate = 10 mM D-ribulose 5-phosphate, +10 micromol/l ZnCl2
additional information