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5.1.3.1: ribulose-phosphate 3-epimerase

This is an abbreviated version!
For detailed information about ribulose-phosphate 3-epimerase, go to the full flat file.

Word Map on EC 5.1.3.1

Reaction

D-ribulose 5-phosphate
=
D-xylulose 5-phosphate

Synonyms

cyt-RPEase, D-ribulose 5-phosphate 3-epimerase, D-Ribulose phosphate-3-epimerase, D-Ribulose-5-P 3-epimerase, D-ribulose-5-phosphate 3-epimerase, D-Ribulose-5-phosphate epimerase, D-ribulose-5-phosphate-3-epimerase, D-Xylulose-5-phosphate 3-epimerase, Epimerase, ribulose phosphate 3-, Pentose-5-phosphate 3-epimerase, Phosphoketopentose 3-epimerase, Phosphoketopentose epimerase, Phosphoribulose epimerase, PPE, R5P3E, Ribulose 5-phosphate 3-epimerase, Ribulose phosphate 3-epimerase, ribulose-5-phosphate 3-epimerase, RPE, rpe1, rpe1-MGA3, rpe1-PB1, rpe2, rpe2-MGA3, RPEase, rpeC, rpeP, Ru5P epimerase, Ru5Pepimerase, slr1622, Xylulose phosphate 3-epimerase, xylulose-5-P 3-epimerase

ECTree

     5 Isomerases
         5.1 Racemases and epimerases
             5.1.3 Acting on carbohydrates and derivatives
                5.1.3.1 ribulose-phosphate 3-epimerase

Crystallization

Crystallization on EC 5.1.3.1 - ribulose-phosphate 3-epimerase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method, using 25% (w/v) PEG 3350, 100 mM Bis-Tris (pH 5.5), and 200 mM NaCl
crystals are produced with hanging drop method using 6 mg/ml cytosolic enzyme, 1.0 M ammonium sulfate, 50 mM Mops, pH 7.5, 1% w/v polyethylene glycol 400, 10 mM glycerol-3-phosphate and 5 mM L-arginine in the drop
hanging drop vapor diffusion method
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crystal structure of the RPE is solved at 1.8 A resolution in the presence of D-xylitol 5-phosphate, an inert analogue of the D-xylulose 5-phosphate substrate. This structure suggests that the 2,3-enediolate intermediate in the 1,1-proton transfer reaction is stabilized by bidentate coordination to the Zn2+ that also is liganded to His 34, Asp 36, His 67, and Asp 176, the carboxylate groups of the Asp residues are positioned also to function as the acid/base catalysts
crystals are grown using the microbatch technique from 22% methyl ether PEG 2000, 200 mM MgCl2, 100 mM MES pH 6.0. The crystals belong tp space group P2(1), with unit-cell parameters a = 82.6 A, b = 87.8 A, c = 97.8 A, beta = 114.6°, 1.6 A resolution
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