5.1.2.2: mandelate racemase
This is an abbreviated version!
For detailed information about mandelate racemase, go to the full flat file.
Word Map on EC 5.1.2.2
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5.1.2.2
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s-mandelate
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kenyon
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alpha-proton
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muconate
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gerlt
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1,1-proton
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benzohydroxamate
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petsko
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kozarich
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mitra
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galactarate
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benzoylformate
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d-glucarate
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ransom
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synthesis
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analysis
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biotechnology
- 5.1.2.2
- s-mandelate
-
kenyon
-
alpha-proton
- muconate
-
gerlt
-
1,1-proton
- benzohydroxamate
-
petsko
-
kozarich
- mitra
- galactarate
- benzoylformate
- d-glucarate
-
ransom
- synthesis
- analysis
- biotechnology
Reaction
Synonyms
mandelate racemase, mandelic acid racemase, mdlA, MR, racemase, mandelate
ECTree
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Metals Ions
Metals Ions on EC 5.1.2.2 - mandelate racemase
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Co2+
Divalent metal ions
Fe2+
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absolute requirement for a divalent metal ion: Mg2+, Mn2+, Ni2+, Co2+ or Fe2+
Mg2+
Mn2+
Ni2+
Co2+
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Mg2+, Mn2+, Ni2+, Co2+ or Fe2+ satisfy the divalent metal ion requirement
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increase the affinity of the enzyme for the substrate. No absolute requirement
Divalent metal ions
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the required divalent metal ion is ligated by the carboxyl groups of Asp195, Glu221, and Glu247, which emanate from the carboxy-terminal ends of strands 3, 4 and 5, respectively
Mg2+
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Mg2+, Mn2+, Ni2+, Co2+ or Fe2+ satisfy the divalent metal ion requirement
Mg2+
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Km: 0.65 mM for wild-type enzyme and 0.34 mM for mutant enzyme N197A
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Mg2+, Mn2+, Ni2+, Co2+ or Fe2+ satisfy the divalent metal ion requirement
Mn2+
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enzyme binds 0.9 mol Mn2+ per mol of subunit with a dissociation constant of 0.008 mM. Also six additional Mn2+ bind to the enzyme much more weakly, with a dissociation constant of 1.5 mM
Ni2+
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Mg2+, Mn2+, Ni2+, Co2+ or Fe2+ satisfy the divalent metal ion requirement