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1.2 - 4.2
(2R)-2-naphthylglycolate
9.94 - 15.97
(R)-2-chloromandelate
3.5
(R)-2-hydroxy-3-butenoic acid
-
-
0.35 - 4.4
(R)-2-naphthylglycolate
1.1 - 4.4
(R)-2-naphtylglycolate
-
1.27 - 3.92
(R)-3-chloromandelate
1.13 - 1.24
(R)-mandelamide
1.2
(R)-trifluorolactate
pH 7.5, 25°C, recombinant wild-type enzyme
10.62 - 19.91
(S)-2-chloromandelate
0.41 - 2.9
(S)-2-naphthylglycolate
1.74
(S)-trifluorolactate
pH 7.5, 25°C, recombinant wild-type enzyme
0.256
4-Bromo-D-mandelate
-
-
0.29
4-Hydroxy-D-mandelate
-
-
0.33
4-Methoxy-D-mandelate
-
-
additional information
additional information
-
1.2
(2R)-2-naphthylglycolate
pH 7.5, 25°C, wild-type enzyme
1.6
(2R)-2-naphthylglycolate
pH 7.5, 25°C, mutant V29L
3.5
(2R)-2-naphthylglycolate
pH 7.5, 25°C, wild-type enzyme, in presence of 20 mM MgCl2
4.2
(2R)-2-naphthylglycolate
pH 7.5, 25°C, mutant V22I
9.94
(R)-2-chloromandelate
mutant V29I/Y45F, pH 7.5, 25°C
11.66
(R)-2-chloromandelate
mutant Y45F, pH 7.5, 25°C
11.99
(R)-2-chloromandelate
mutant V29I, pH 7.5, 25°C
14.52
(R)-2-chloromandelate
mutant V22I/V29I/Y54F, pH 7.5, 25°C
14.82
(R)-2-chloromandelate
wild-type enzyme, pH 7.5, 25°C
15.1
(R)-2-chloromandelate
mutant V22I/V29I, pH 7.5, 25°C
15.51
(R)-2-chloromandelate
mutant V22I, pH 7.5, 25°C
15.97
(R)-2-chloromandelate
mutant V22I/Y45F, pH 7.5, 25°C
0.35
(R)-2-naphthylglycolate
mutant enzyme F52W/Y54W
0.46
(R)-2-naphthylglycolate
wild-type enzyme
0.46
(R)-2-naphthylglycolate
25°C, pH 7.5
1.1
(R)-2-naphthylglycolate
mutant V29F, pH 7.5, 25°C
1.2
(R)-2-naphthylglycolate
wild-type, pH 7.5, 25°C
1.3
(R)-2-naphthylglycolate
mutant V26L, pH 7.5, 25°C
1.6
(R)-2-naphthylglycolate
mutant V29L, pH 7.5, 25°C
1.7
(R)-2-naphthylglycolate
mutant V26F, pH 7.5, 25°C
1.8
(R)-2-naphthylglycolate
mutant V29A, pH 7.5, 25°C
1.9
(R)-2-naphthylglycolate
mutant A25V, pH 7.5, 25°C
1.9
(R)-2-naphthylglycolate
mutant V22A, pH 7.5, 25°C
2.1
(R)-2-naphthylglycolate
mutant V26A, pH 7.5, 25°C
2.2
(R)-2-naphthylglycolate
mutant V22F, pH 7.5, 25°C
3.2
(R)-2-naphthylglycolate
mutant T24S, pH 7.5, 25°C
3.5
(R)-2-naphthylglycolate
wild-type, pH 7.5, 25°C, presence of 20 mM Mg2+
3.7
(R)-2-naphthylglycolate
mutant V22I/V29L, pH 7.5, 25°C, presence of 20 mM Mg2+
4.2
(R)-2-naphthylglycolate
mutant V22I, pH 7.5, 25°C, presence of 20 mM Mg2+
4.4
(R)-2-naphthylglycolate
mutant V26A/V29L, pH 7.5, 25°C
1.1
(R)-2-naphtylglycolate
pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V29F
-
1.2
(R)-2-naphtylglycolate
pH 7.5 at 25°C, 3.3 mM Mg2+, wild-type
-
1.3
(R)-2-naphtylglycolate
pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V26L
-
1.6
(R)-2-naphtylglycolate
pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V29L
-
1.7
(R)-2-naphtylglycolate
pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V26F
-
1.8
(R)-2-naphtylglycolate
pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V29A
-
1.9
(R)-2-naphtylglycolate
pH 7.5 at 25°C, 3.3 mM Mg2+, mutant A25V
-
1.9
(R)-2-naphtylglycolate
pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V22A
-
2.1
(R)-2-naphtylglycolate
pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V26A
-
2.2
(R)-2-naphtylglycolate
pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V22F
-
3.2
(R)-2-naphtylglycolate
pH 7.5 at 25°C, 3.3 mM Mg2+, mutant T24S
-
3.5
(R)-2-naphtylglycolate
pH 7.5 at 25°C, 20 mM Mg2+, wild-type
-
3.7
(R)-2-naphtylglycolate
pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V22I/V29L
-
4.2
(R)-2-naphtylglycolate
pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V22I
-
4.4
(R)-2-naphtylglycolate
pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V26A/V29L
-
1.27
(R)-3-chloromandelate
pH 7.5, 25°C, mutant V29L
1.6 - 2
(R)-3-chloromandelate
pH 7.5, 25°C, mutant Y54L
1.7 - 2
(R)-3-chloromandelate
pH 7.5, 25°C, mutant L319F
1.87
(R)-3-chloromandelate
pH 7.5, 25°C, mutant V26L
1.89
(R)-3-chloromandelate
pH 7.5, 25°C, wild-type enzyme
1.9
(R)-3-chloromandelate
pH 7.5, 25°C, mutant V26S
1.95
(R)-3-chloromandelate
pH 7.5, 25°C, mutant Y54F
1.99
(R)-3-chloromandelate
pH 7.5, 25°C, mutant V26I/Y54I
2.12
(R)-3-chloromandelate
pH 7.5, 25°C, mutant V22I
2.17
(R)-3-chloromandelate
pH 7.5, 25°C, mutant Y54V
2.22
(R)-3-chloromandelate
pH 7.5, 25°C, mutant Y54A
2.4
(R)-3-chloromandelate
pH 7.5, 25°C, mutant V22L
2.46
(R)-3-chloromandelate
pH 7.5, 25°C, mutant V26I
3.02
(R)-3-chloromandelate
pH 7.5, 25°C, mutant Y54I
3.92
(R)-3-chloromandelate
pH 7.5, 25°C, mutant V26I/Y54V
1.13
(R)-mandelamide
pH 7.5, 25°C, wild-type enzyme
1.24
(R)-mandelamide
pH 7.5, 25°C, mutant V26L
0.29
(R)-mandelate
mutant enzyme F52W/Y64W
0.34
(R)-mandelate
-
wild type
0.4
(R)-mandelate
-
wild type enzyme
0.44
(R)-mandelate
-
pH 7.5
0.7
(R)-mandelate
wild-type enzyme
0.81
(R)-mandelate
-
25°C, pH 7.5, wild-type enzyme
0.9
(R)-mandelate
mutant V29F, pH 7.5, 25°C
0.9
(R)-mandelate
pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V29F
0.91
(R)-mandelate
mutant V26A, pH 7.5, 25°C
0.91
(R)-mandelate
pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V26A
0.95
(R)-mandelate
-
pH 6.3
1.1
(R)-mandelate
mutant enzyme Y54W
1.1
(R)-mandelate
mutant A25V, pH 7.5, 25°C
1.1
(R)-mandelate
pH 7.5 at 25°C, 3.3 mM Mg2+, mutant A25V
1.2
(R)-mandelate
pH 7.5, 25°C, recombinant wild-type enzyme
1.2
(R)-mandelate
-
pH 7.5, 25°C, recombinant wild-type enzyme
1.2
(R)-mandelate
wild-type, pH 7.5, 25°C
1.2
(R)-mandelate
pH 7.5 at 25°C, 3.3 mM Mg2+, wild-type
1.4
(R)-mandelate
mutant V26A/V29L, pH 7.5, 25°C
1.4
(R)-mandelate
pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V26A/V29L
1.47
(R)-mandelate
pH 7.5 at 25°C, 20 mM Mg2+, wild-type
1.47
(R)-mandelate
wild-type, pH 7.5, 25°C, presence of 20 mM Mg2+
1.5
(R)-mandelate
D270N mutant
1.7
(R)-mandelate
mutant V26L, pH 7.5, 25°C
1.7
(R)-mandelate
mutant V29L, pH 7.5, 25°C
1.7
(R)-mandelate
pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V26L
1.7
(R)-mandelate
pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V29L
1.8
(R)-mandelate
mutant V26F, pH 7.5, 25°C
1.8
(R)-mandelate
pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V26F
2.02
(R)-mandelate
-
pH 8.7
2.4
(R)-mandelate
-
E317Q mutant
2.5
(R)-mandelate
mutant enzyme F52W
2.8
(R)-mandelate
mutant T24S, pH 7.5, 25°C
2.8
(R)-mandelate
mutant V22I/V29L, pH 7.5, 25°C, presence of 20 mM Mg2+
2.8
(R)-mandelate
pH 7.5 at 25°C, 3.3 mM Mg2+, mutant T24S
2.8
(R)-mandelate
pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V22I/V29L
2.9
(R)-mandelate
mutant V22I, pH 7.5, 25°C, presence of 20 mM Mg2+
2.9
(R)-mandelate
pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V22I
4.4
(R)-mandelate
mutant V22A, pH 7.5, 25°C
4.4
(R)-mandelate
mutant V22F, pH 7.5, 25°C
4.4
(R)-mandelate
pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V22A
4.4
(R)-mandelate
pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V22F
5
(R)-mandelate
mutant V29A, pH 7.5, 25°C
5
(R)-mandelate
pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V29A
5.4
(R)-mandelate
-
25°C, pH 7.5, mutant enzyme N197A
10.62
(S)-2-chloromandelate
mutant V29I/Y45F, pH 7.5, 25°C
10.9
(S)-2-chloromandelate
mutant Y45F, pH 7.5, 25°C
13.74
(S)-2-chloromandelate
mutant V22I/V29I, pH 7.5, 25°C
14.19
(S)-2-chloromandelate
mutant V22I/Y45F, pH 7.5, 25°C
14.35
(S)-2-chloromandelate
mutant V29I, pH 7.5, 25°C
17.74
(S)-2-chloromandelate
wild-type enzyme, pH 7.5, 25°C
17.94
(S)-2-chloromandelate
mutant V22I/V29I/Y54F, pH 7.5, 25°C
19.91
(S)-2-chloromandelate
mutant V22I, pH 7.5, 25°C
0.41
(S)-2-naphthylglycolate
wild-type enzyme
0.41
(S)-2-naphthylglycolate
25°C, pH 7.5
0.55
(S)-2-naphthylglycolate
mutant V29F, pH 7.5, 25°C
0.55
(S)-2-naphthylglycolate
pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V29F
0.62
(S)-2-naphthylglycolate
mutant V22F, pH 7.5, 25°C
0.62
(S)-2-naphthylglycolate
mutant V26L, pH 7.5, 25°C
0.62
(S)-2-naphthylglycolate
pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V22F
0.62
(S)-2-naphthylglycolate
pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V26L
0.74
(S)-2-naphthylglycolate
mutant V26A, pH 7.5, 25°C
0.74
(S)-2-naphthylglycolate
pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V26A
0.8
(S)-2-naphthylglycolate
mutant V29L, pH 7.5, 25°C
0.8
(S)-2-naphthylglycolate
pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V29L
0.93
(S)-2-naphthylglycolate
mutant V29A, pH 7.5, 25°C
0.93
(S)-2-naphthylglycolate
pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V29A
1
(S)-2-naphthylglycolate
wild-type, pH 7.5, 25°C
1
(S)-2-naphthylglycolate
pH 7.5 at 25°C, 3.3 mM Mg2+, wild-type
1.1
(S)-2-naphthylglycolate
mutant V26F, pH 7.5, 25°C
1.1
(S)-2-naphthylglycolate
pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V26F
1.4
(S)-2-naphthylglycolate
mutant T24S, pH 7.5, 25°C
1.4
(S)-2-naphthylglycolate
pH 7.5 at 25°C, 3.3 mM Mg2+, mutant T24S
1.5
(S)-2-naphthylglycolate
mutant enzyme F52W/Y54W
1.55
(S)-2-naphthylglycolate
mutant V22A, pH 7.5, 25°C
1.55
(S)-2-naphthylglycolate
pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V22A
1.6
(S)-2-naphthylglycolate
pH 7.5 at 25°C, 20 mM Mg2+, wild-type
1.6
(S)-2-naphthylglycolate
wild-type, pH 7.5, 25°C, presence of 20 mM Mg2+
1.67
(S)-2-naphthylglycolate
mutant A25V, pH 7.5, 25°C
1.67
(S)-2-naphthylglycolate
pH 7.5 at 25°C, 3.3 mM Mg2+, mutant A25V
1.7
(S)-2-naphthylglycolate
mutant V26A/V29L, pH 7.5, 25°C
1.7
(S)-2-naphthylglycolate
pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V26A/V29L
2.4
(S)-2-naphthylglycolate
mutant V22I/V29L, pH 7.5, 25°C, presence of 20 mM Mg2+
2.4
(S)-2-naphthylglycolate
pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V26A/V29L
2.9
(S)-2-naphthylglycolate
mutant V22I, pH 7.5, 25°C, presence of 20 mM Mg2+
2.9
(S)-2-naphthylglycolate
pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V22I
0.3
(S)-Mandelate
recombinant mandelate racemase, putative misfolded form with N-terminal hexahistidine tag, Na-HEPES buffer (0.1 M, pH 7.5), 3.3 mM MgCl2
0.35
(S)-Mandelate
-
wild type
0.54
(S)-Mandelate
wild-type enzyme
0.59
(S)-Mandelate
-
mutant K166R
0.6
(S)-Mandelate
mutant V29L, pH 7.5, 25°C
0.6
(S)-Mandelate
pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V29L
0.6
(S)-Mandelate
recombinant mrIII fusion protein bearing an N-terminal StrepII-tag and a C-terminal decahistidine tag, Na-HEPES buffer (0.1 M, pH 7.5), 3.3 mM MgCl2
0.62
(S)-Mandelate
-
25°C, pH 7.5, wild-type enzyme
0.8
(S)-Mandelate
recombinant mandelate racemase represents correctly folded enzyme with N-terminal hexahistidine tag, in Na-HEPES buffer (0.1 M, pH 7.5),3.3 mM MgCl2
0.97
(S)-Mandelate
wild-type, pH 7.5, 25°C
0.97
(S)-Mandelate
pH 7.5 at 25°C, 3.3 mM Mg2+, wild-type
1
(S)-Mandelate
pH 7.5, 25°C, recombinant wild-type enzyme
1
(S)-Mandelate
-
pH 7.5, 25°C, recombinant wild-type enzyme
1.07
(S)-Mandelate
pH 7.5 at 25°C, 20 mM Mg2+, wild-type
1.07
(S)-Mandelate
wild-type, pH 7.5, 25°C, presence of 20 mM Mg2+
1.1
(S)-Mandelate
mutant V26A/V29L, pH 7.5, 25°C
1.1
(S)-Mandelate
mutant V26F, pH 7.5, 25°C
1.1
(S)-Mandelate
mutant V26L, pH 7.5, 25°C
1.1
(S)-Mandelate
mutant V29F, pH 7.5, 25°C
1.1
(S)-Mandelate
pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V26A/V29L
1.1
(S)-Mandelate
pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V26F
1.1
(S)-Mandelate
pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V26L
1.1
(S)-Mandelate
pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V29F
1.1
(S)-Mandelate
recombinant mandelate racemase, replacement of the N-terminal hexahistidine tag by a StrepII-tag appears to ameliorate the folding problem yielding a single enzyme species, Na-HEPES buffer (0.1 M, pH 7.5), 3.3 mM MgCl2
1.4
(S)-Mandelate
-
mutant K166R
1.4
(S)-Mandelate
-
25°C, pH 7.5, mutant enzyme N197A
1.5
(S)-Mandelate
mutant V22I/V29L, pH 7.5, 25°C, presence of 20 mM Mg2+
1.5
(S)-Mandelate
pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V26A/V29L
1.7
(S)-Mandelate
mutant V22I, pH 7.5, 25°C, presence of 20 mM Mg2+
1.7
(S)-Mandelate
mutant V26A, pH 7.5, 25°C
1.7
(S)-Mandelate
pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V22I
1.7
(S)-Mandelate
pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V26A
1.8
(S)-Mandelate
mutant A25V, pH 7.5, 25°C
1.8
(S)-Mandelate
pH 7.5 at 25°C, 3.3 mM Mg2+, mutant A25V
2.1
(S)-Mandelate
D270N mutant
2.9
(S)-Mandelate
mutant enzyme Y54W
3
(S)-Mandelate
mutant T24S, pH 7.5, 25°C
3
(S)-Mandelate
pH 7.5 at 25°C, 3.3 mM Mg2+, mutant T24S
3.3
(S)-Mandelate
mutant enzyme F52W
3.4
(S)-Mandelate
mutant V22A, pH 7.5, 25°C
3.4
(S)-Mandelate
pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V22A
3.8
(S)-Mandelate
mutant enzyme F52W/Y64W
3.9
(S)-Mandelate
mutant V29A, pH 7.5, 25°C
3.9
(S)-Mandelate
pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V29A
6.1
(S)-Mandelate
mutant V22F, pH 7.5, 25°C
6.1
(S)-Mandelate
pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V22F
0.1
D(-)-mandelate
-
in presence of 1 mM Mg2+
0.1
D(-)-mandelate
-
4-chloro-D-mandelate
0.093
D-mandelate
-
-
0.25
D-mandelate
-
L-mandelate
0.04
Mg2+
pH 7.5, mutant V22A, addition of either 10 mM (R)- or (S)-mandelate
0.04
Mg2+
pH 7.5, mutant V22F, addition of either 10 mM (R)- or (S)-mandelate
0.05
Mg2+
pH 7.5, mutant V26F, addition of either 10 mM (R)- or (S)-mandelate
0.08
Mg2+
pH 7.5, mutant V29A, addition of either 10 mM (R)- or (S)-mandelate
0.12
Mg2+
pH 7.5, mutant V26L, addition of either 10 mM (R)- or (S)-mandelate
0.15
Mg2+
pH 7.5, mutant V26A, addition of either 10 mM (R)- or (S)-mandelate
0.22
Mg2+
pH 7.5, mutant V29F, addition of either 10 mM (R)- or (S)-mandelate
0.29
Mg2+
pH 7.5, mutant T24S, addition of either 10 mM (R)- or (S)-mandelate
0.34
Mg2+
pH 7.5, wild-type, addition of either 10 mM (R)- or (S)-mandelate
0.47
Mg2+
pH 7.5, mutant V29L, addition of either 10 mM (R)- or (S)-mandelate
0.51
Mg2+
pH 7.5, mutant V26A/V29L, addition of either 10 mM (R)- or (S)-mandelate
0.64
Mg2+
pH 7.5, mutant A25V, addition of either 10 mM (R)- or (S)-mandelate
3.8
Mg2+
pH 7.5, mutant V22I, addition of either 10 mM (R)- or (S)-mandelate
4.2
Mg2+
pH 7.5, mutant V22I/V29L, addition of either 10 mM (R)- or (S)-mandelate
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics
-
5.1.2.2 mandelate racemase
results (
results (
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