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5.1.1.4: proline racemase

This is an abbreviated version!
For detailed information about proline racemase, go to the full flat file.

Word Map on EC 5.1.1.4

Reaction

L-proline
=
D-proline

Synonyms

CdPRAC, CdProR, FaProR, HjProR, PA45-A, PA45-B, PRAC, PRAC1, PrdF, proline racemase, proline racemase A, proline racemase B, proline racemase/hydroxyproline epimerase, ProR, ProR/HypE, Racemase, proline, TcPA45, TcPRAC, TcPRACA, TcPRACB, Tgr.146.1080, TryPRAC, TvHYP1, TvPRAC

ECTree

     5 Isomerases
         5.1 Racemases and epimerases
             5.1.1 Acting on amino acids and derivatives
                5.1.1.4 proline racemase

Cloned

Cloned on EC 5.1.1.4 - proline racemase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned in Escherichia coli as a 6xHis-tag fusion protein
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, genotyping and genetic organization, detailed overview
DNA and amino acid sequence determination and analysis, sequence comparisons and sequence comparisons and phylogenetic analysis, genotyping and genetic organization, detailed overview
-
expressed in Escherichia coli BL21(DE3) cells
-
expression in Escherichia coli
expression in Escherichia coli BL21 (DE3) with a C-terminal 6-His tag
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
-
TcPRACA is expressed in Escherichia coli BL21 (DE3)
the paralogous genes TcPRACA (A+) and TcPRACB (B++) are overexpressed in non-infective epimastigote forms using appropriate vectors to obtain stable chromosomal integration of these genes in sense and antisense orientations