5.1.1.13: aspartate racemase

This is an abbreviated version!
For detailed information about aspartate racemase, go to the full flat file.

Word Map on EC 5.1.1.13

Reaction

L-aspartate
=
D-Aspartate

Synonyms

Racemase, aspartate, Aspartate racemase, D-Aspartate racemase, P. AspR, PhAspR, D-Asp racemase, AspR, Asp racemase, GOT1L1, pyridoxal 5'-phosphate-independent aspartate racemase, PTO0149, PtoAspR, LsAspR, SbAspR, pyridoxal 5'-phosphate independent aspartate racemase, OCC_11152, Tl-AspR, PLP-independent AspR

ECTree

     5 Isomerases
         5.1 Racemases and epimerases
             5.1.1 Acting on amino acids and derivatives
                5.1.1.13 aspartate racemase

Engineering

Engineering on EC 5.1.1.13 - aspartate racemase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
P150S
JT020910
PCR-based site-directed mutagenesis, the mutant shows reduced aspartate and serine racemase activities compared to the wild-type enzyme
S151P/S152F
JT020910
PCR-based site-directed mutagenesis, the mutant is inactive as aspartate racemase, but shows serine racemase activity, EC 5.1.1.18
S150P/S151P/S152Y
PCR-based site-directed mutagenesis, the SSS to PPY mutation of Crassostrea AspR triple serine loop region drastically decreases the specific AspR activity to below 0.04% compared to wild-type, whereas it significantly increases the specific SerR and SDH activities by 6-13fold. The large change in the AspR activity in the mutant enzyme is caused by approximately 400fold decrease in kcat rather than by 22-32fold decrease in Km
K136W
-
the importance of lysine-136, the presumed proton donor, is highlighted by its mutation to tryptophan, which virtually abolishes racemase activity
C83A
site-directed mutagenesis, inactive mutant
C194A
site-directed mutagenesis, inactive mutant
C83A/C194A
site-directed mutagenesis, inactive mutant
C83A
Thermococcus litoralis ATCC 51850 / DSM 5473 / JCM 8560 / NS-C
-
site-directed mutagenesis, inactive mutant
-
C194A
Thermococcus litoralis ATCC 51850 / DSM 5473 / JCM 8560 / NS-C
-
site-directed mutagenesis, inactive mutant
-
C83A/C194A
Thermococcus litoralis ATCC 51850 / DSM 5473 / JCM 8560 / NS-C
-
site-directed mutagenesis, inactive mutant
-
additional information