5.1.1.13: aspartate racemase
This is an abbreviated version!
For detailed information about aspartate racemase, go to the full flat file.
Word Map on EC 5.1.1.13
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5.1.1.13
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l-aspartate
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d-amino
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racemization
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asprs
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5'-phosphate-dependent
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horikoshii
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scapharca
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d-serine
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broughtonii
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d-forms
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d-enantiomer
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serrs
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plp-dependent
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d-glutamate
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mirror-symmetric
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food industry
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analysis
- 5.1.1.13
- l-aspartate
-
d-amino
-
racemization
- asprs
-
5'-phosphate-dependent
- horikoshii
-
scapharca
- d-serine
- broughtonii
-
d-forms
-
d-enantiomer
- serrs
-
plp-dependent
- d-glutamate
-
mirror-symmetric
- food industry
- analysis
Reaction
Synonyms
Asp racemase, Aspartate racemase, AspR, D-Asp racemase, D-Aspartate racemase, GOT1L1, LsAspR, OCC_11152, P. AspR, PhAspR, PLP-independent AspR, PTO0149, PtoAspR, pyridoxal 5'-phosphate independent aspartate racemase, pyridoxal 5'-phosphate-independent aspartate racemase, Racemase, aspartate, SbAspR, Tl-AspR
ECTree
Advanced search results
Engineering
Engineering on EC 5.1.1.13 - aspartate racemase
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P150S
JT020910
PCR-based site-directed mutagenesis, the mutant shows reduced aspartate and serine racemase activities compared to the wild-type enzyme
S151P/S152F
JT020910
PCR-based site-directed mutagenesis, the mutant is inactive as aspartate racemase, but shows serine racemase activity, EC 5.1.1.18
S150P/S151P/S152Y
PCR-based site-directed mutagenesis, the SSS to PPY mutation of Crassostrea AspR triple serine loop region drastically decreases the specific AspR activity to below 0.04% compared to wild-type, whereas it significantly increases the specific SerR and SDH activities by 6-13fold. The large change in the AspR activity in the mutant enzyme is caused by approximately 400fold decrease in kcat rather than by 22-32fold decrease in Km
K136W
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the importance of lysine-136, the presumed proton donor, is highlighted by its mutation to tryptophan, which virtually abolishes racemase activity
C82A
C194A
Thermococcus litoralis ATCC 51850 / DSM 5473 / JCM 8560 / NS-C
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site-directed mutagenesis, inactive mutant
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C83A
Thermococcus litoralis ATCC 51850 / DSM 5473 / JCM 8560 / NS-C
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site-directed mutagenesis, inactive mutant
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C83A/C194A
Thermococcus litoralis ATCC 51850 / DSM 5473 / JCM 8560 / NS-C
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site-directed mutagenesis, inactive mutant
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additional information
C82A
catalytically inactive. Crystallization data in complex with citric acid
C82A
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catalytically inactive. Crystallization data in complex with citric acid
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JT020910
substituting the triple serine loop region in AspRs enhances serine racemization
additional information
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the cDNA included an open reading frame of 1017 bp encoding a protein of 338 amino acids, and the deduced amino acid sequence contains a pyridoxal 5'-phosphate-binding motif
additional information
substituting the triple serine loop region in AspRs enhances serine racemization
additional information
generation of Got1l1 knockout mice. Got1l1 mRNA is highly expressed in the testis in wild-type mice, but is not detected in Got1l1 knockout mice. The D-aspartate contents of knockout and Got1l1 knockout mice are not significantly different in the testis and hippocampus
additional information
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generation of Got1l1 knockout mice. Got1l1 mRNA is highly expressed in the testis in wild-type mice, but is not detected in Got1l1 knockout mice. The D-aspartate contents of knockout and Got1l1 knockout mice are not significantly different in the testis and hippocampus
additional information
substituting the triple serine loop region in AspRs enhances serine racemization