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5.1.1.13: aspartate racemase

This is an abbreviated version!
For detailed information about aspartate racemase, go to the full flat file.

Word Map on EC 5.1.1.13

Reaction

L-aspartate
=
D-Aspartate

Synonyms

Asp racemase, Aspartate racemase, AspR, D-Asp racemase, D-Aspartate racemase, GOT1L1, LsAspR, OCC_11152, P. AspR, PhAspR, PLP-independent AspR, PTO0149, PtoAspR, pyridoxal 5'-phosphate independent aspartate racemase, pyridoxal 5'-phosphate-independent aspartate racemase, Racemase, aspartate, SbAspR, Tl-AspR

ECTree

     5 Isomerases
         5.1 Racemases and epimerases
             5.1.1 Acting on amino acids and derivatives
                5.1.1.13 aspartate racemase

Cloned

Cloned on EC 5.1.1.13 - aspartate racemase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned in Escherichia coli
-
enzyme is cloned into pET vector, which is transformed into BL21 Escherichia coli
-
expression in Escherichia coli
expression in Synechocystes PCC 6803 with a His6-tag
-
gene Got1l1, real-time PCR expression analysis
-
gene Got1l1, real-time PCR expression analysis, relationships between the D-Asp content and expression levels of Got1l1 and Ddo mRNAs in cultured rat cells
-
gene Got1l1, recombinant expression of His-tagged wild-type enzyme in Escherichia coli strain BL21 and in HEK-293T cells
gene OCC_11152, sequence comparisons, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain Rosetta (DE3)
overexpression in Escherichia coli
-
recombinant enzyme expression in Escherichia coli strain BL21(DE3)
recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
recombinant expression of C-terminally His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21 (DE3)
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS
-
sequence comparisons and phylogenetic tree of serine and aspartate racemases, recombinant expression of C-terminally His-tagged wild-type enzyme in Escherichia coli strain BL21(DE3)