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4.6.1.12: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

This is an abbreviated version!
For detailed information about 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, go to the full flat file.

Word Map on EC 4.6.1.12

Reaction

2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
=
2-C-methyl-D-erythritol 2,4-cyclodiphosphate
 +
CMP

Synonyms

2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, 2-C-methyl-D-erythritol-2,4-cyclodiphosphate synthase, 2-methylerythritol 2,4-cyclodiphosphate synthase, 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, 2C-methyl-D-erythritol-2,4-cyclodiphosphate synthase, 2C-methyl-D-erythrol-2,4-cyclodiphosphate synthase, cMEPP synthase, IspDF, IspE, IspF, MCS, MDS, ME-CPP synthase, MEC synthase, MECDP synthase, MECDP-synthase, MECP, MECPS, MECS, YGBB, YgbB protein

ECTree

     4 Lyases
         4.6 Phosphorus-oxygen lyases
             4.6.1 Phosphorus-oxygen lyases (only sub-subclass identified to date)
                4.6.1.12 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Cloned

Cloned on EC 4.6.1.12 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a 339 bp core fragment and full-length DNA of MECPS amplified and subcloned into pGEM T-easy vector. Escherichia coli harboring both of the plasmids, pAC-BETA and pTrc or pTrcTmMECPS or pTrcAtMECPS
amplification product digested with BamHI and PstI and ligated into plasmid pQE30, expressed in Escherichia coli strain XL1-Blue
cloned into pET17b for expression of the native polypeptide in Escherichia coli strain BL21
expressed in Escherichia coli
expressed in Escherichia coli strain BL21 (DE3)
expressed in Escherichia coli strain EB370
expression in Escherichia coli
expression in Escherichia coli strain NMW26 and Arabidopsis thaliana
final amplification product digested with the restriction endonucleases BamHI and SalI and cloned into plasmid pQE30, expressed in Escherichia coli strain XL1-Blue