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0.54
(+)-methiin
mutant C115H, pH pH 8.0, 30°C
1.4
(+)-propiin
mutant C115H, pH pH 8.0, 30°C
0.42 - 1.79
(+)-S-(2-propenyl)-L-cysteine sulfoxide
1.26
(+-)-alliin
mutant C115H, pH pH 8.0, 30°C
0.47
(+-)-ethiin
mutant C115H, pH pH 8.0, 30°C
0.57
(+-)-methiin
mutant C115H, pH pH 8.0, 30°C
1.1
(+-)-propiin
mutant C115H, pH pH 8.0, 30°C
1
(-)-alliin
-
about, below, pH 7.0, 30°C
28.6
(2R)-2-amino-3-(1-phenylethylsulfinyl)propanoic acid
-
pH 8.0, 22°C
9.29
(2R)-2-amino-3-(2-methylbenzylsulfinyl)propanoic acid
-
pH 8.0, 22°C
2.11
(2R)-2-amino-3-(4-chlorobenzylsulfinyl)propanoic acid
-
pH 8.0, 22°C
0.72
(2R)-2-amino-3-(4-methylbenzylsulfinyl)propanoic acid
-
pH 8.0, 22°C
0.33
(2R)-2-amino-3-(ethylsulfinyl)propanoic acid
-
pH 8.0, 22°C
0.23
(2R)-2-amino-3-(phenethylsulfinyl)propanoic acid
-
pH 8.0, 22°C
1.96
(2R)-2-amino-3-(phenylsulfinyl)propanoic acid
-
pH 8.0, 22°C
0.5
2-hydroxyethiin
-
pH 8.0, 22°C
1.32 - 1.418
4-mercaptopyridine
0.06
desglutamyl-lentinic acid
-
-
2.8
L-(-)-alliin
-
enzyme from garlic powder
0.39
petiveriin
-
pH 8.0, 22°C
0.25
propiin
-
pH 8.0, 22°C
0.4 - 0.5
S-(2-chloro-4-nitrophenyl)-L-Cys
0.4 - 0.7
S-(2-chloro-6-nitrophenyl)-L-Cys
1.1 - 24.1
S-allyl-L-Cys sulfoxide
2.5 - 68.4
S-ethyl-L-Cys sulfoxide
5.8
S-ethyl-L-cysteine sulfoxide
-
-
47 - 96.6
S-methyl-L-Cys sulfoxide
7.6 - 9.9
S-phenyl-L-Cys sulfoxide
2.6 - 3
S-propenyl-L-Cys sulfoxide
0.6 - 22
S-propyl-L-Cys sulfoxide
additional information
additional information
-
1.2
(+)-alliin
mutant C115H, pH pH 8.0, 30°C
5.7
(+)-alliin
-
pH 7.0, 30°C
0.42
(+)-S-(2-propenyl)-L-cysteine sulfoxide
-
pH 7.0, 25°C
0.47
(+)-S-(2-propenyl)-L-cysteine sulfoxide
-
pH 7.0, 25°C
0.68
(+)-S-(2-propenyl)-L-cysteine sulfoxide
-
pH 7.0, 25°C
0.77
(+)-S-(2-propenyl)-L-cysteine sulfoxide
-
pH 7.0, 25°C
0.85
(+)-S-(2-propenyl)-L-cysteine sulfoxide
-
pH 7.0, 25°C
1.25
(+)-S-(2-propenyl)-L-cysteine sulfoxide
-
pH 7.0, 25°C
1.48
(+)-S-(2-propenyl)-L-cysteine sulfoxide
-
pH 7.0, 25°C
1.59
(+)-S-(2-propenyl)-L-cysteine sulfoxide
-
pH 7.0, 25°C
1.79
(+)-S-(2-propenyl)-L-cysteine sulfoxide
-
pH 7.0, 25°C
1.493
(+/-)-alliin
-
native enzyme, pH 7.0, 35°C
1.521
(+/-)-alliin
-
catalytic alliinase-specific variable domain of the heavy chain of a heavy-chain antibody VHH C-10, pH 7.0, 35°C
2.3
(+/-)-alliin
-
pH 7.0, 30°C
1.32
4-mercaptopyridine
-
catalytic alliinase-specific variable domain of the heavy chain of a heavy-chain antibody VHH C-10, pH 7.0, 35°C
1.418
4-mercaptopyridine
-
native enzyme, pH 7.0, 35°C
0.44
alliin
mutant C115H, pH 8.0, 30°C
0.83
alliin
-
pH 7.0, 30°C
1.75
alliin
mutant C115A, pH 8.0, 30°C
4.22
alliin
-
pH 8.0, 22°C
2.2
cystine
-
alliinase I
9.7
cystine
-
alliinase II
0.457
ethiin
mutant C115H, pH 8.0, 30°C
5.48
ethiin
mutant C115A, pH 8.0, 30°C
0.88
L-(+)-alliin
Allium cepa * Allium saxatile
-
-
1.04
L-(+)-alliin
Allium cepa * Allium globosum
-
-
1.44
L-(+)-alliin
Allium cepa * Allium senescens
-
-
1.6
L-(+)-alliin
-
enzyme from garlic powder
1.92
L-(+)-alliin
Allium cepa * Allium oliquum
-
-
2.33
L-(+)-alliin
Allium cepa * Allium chevsuricum
-
-
3.08
L-(+)-alliin
Allium cepa * Allium altyncolicum
-
-
1.94
L-cystine
-
homogenous alliinase prepared from fresh garlic cloves
2.65
L-cystine
-
preparation obtained from garlic powder, activity calculated from the amount of enzymatically formed pyruvate
0.57
methiin
mutant C115H, pH 8.0, 30°C
0.63
methiin
mutant C115A, pH 8.0, 30°C
13.9
methiin
-
pH 8.0, 22°C
0.4
S-(2-chloro-4-nitrophenyl)-L-Cys
-
alliin lyase II
0.5
S-(2-chloro-4-nitrophenyl)-L-Cys
-
alliin lyase I
0.4
S-(2-chloro-6-nitrophenyl)-L-Cys
-
alliin lyase II
0.7
S-(2-chloro-6-nitrophenyl)-L-Cys
-
alliin lyase I
1.1
S-allyl-L-Cys sulfoxide
-
-
2.2
S-allyl-L-Cys sulfoxide
-
-
3.3
S-allyl-L-Cys sulfoxide
-
-
5.8
S-allyl-L-Cys sulfoxide
-
-
10
S-allyl-L-Cys sulfoxide
-
-
10
S-allyl-L-Cys sulfoxide
-
alliin lyase I
11
S-allyl-L-Cys sulfoxide
-
alliin lyase II
18.3
S-allyl-L-Cys sulfoxide
-
alliinase I
24.1
S-allyl-L-Cys sulfoxide
-
alliinase II
2.5
S-ethyl-L-Cys sulfoxide
-
-
2.7
S-ethyl-L-Cys sulfoxide
-
-
4.2
S-ethyl-L-Cys sulfoxide
-
-
4.9
S-ethyl-L-Cys sulfoxide
-
-
5.7
S-ethyl-L-Cys sulfoxide
-
-
13
S-ethyl-L-Cys sulfoxide
-
-
16
S-ethyl-L-Cys sulfoxide
-
alliin lyase I
24
S-ethyl-L-Cys sulfoxide
-
alliin lyase II
45.7
S-ethyl-L-Cys sulfoxide
-
alliinase I
68.4
S-ethyl-L-Cys sulfoxide
-
alliinase II
47
S-methyl-L-Cys sulfoxide
-
alliinase I
55
S-methyl-L-Cys sulfoxide
-
-
96.6
S-methyl-L-Cys sulfoxide
-
alliinase II
7.6
S-phenyl-L-Cys sulfoxide
-
alliin lyase I
9.9
S-phenyl-L-Cys sulfoxide
-
alliin lyase II
2.6
S-propenyl-L-Cys sulfoxide
-
alliinase I
3
S-propenyl-L-Cys sulfoxide
-
alliinase II
0.6
S-propyl-L-Cys sulfoxide
-
alliin lyase I
1.1
S-propyl-L-Cys sulfoxide
-
-
2.1
S-propyl-L-Cys sulfoxide
-
alliin lyase II
16.3
S-propyl-L-Cys sulfoxide
-
alliinase II
22
S-propyl-L-Cys sulfoxide
-
alliinase I
additional information
additional information
-
Km and Vmax vary as a function of substrate structure, with the most preferred substrates being the naturally occurring Petiveria alliacea compounds S-benzyl-L-cysteine sulfoxide and S-2-hydroxyethyl-L-cysteine sulfoxide
-
additional information
additional information
-
kinetic analysis of the alliin/alliinase system using a fiber-optic in situ mon-itoring system (fiber-optic drug dissolution test system, FODT) to explore the application of a fiber-opticsensor analysis technique in the process of monitoring catalytic kinetics, method and mathematical modeling, overview
-
4.4.1.4 alliin lyase
results (
results (
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