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0.5
2,4-dinitrophenyl-L-cysteine
0.013
3-hydroxy-kynurenine
-
-
0.6
beta-chloro-L-alanine
-
-
2.6
beta-chloroalanine
-
-
1
cystine
-
estimated from steady-state measurements
0.04 - 15
L-cystathionine
4.5
L-homolanthionine
-
-
0.83
L-meso-lanthionine
-
-
0.35
L-selenocystathionine
-
-
6.5
O-methyl-DL-serine
-
-
0.008
pyridoxal phosphate
-
-
8.4
S-(1,1,2,2-tetrafluoroethyl)-L-cysteine
-
23°C, pH 7.4
1.36 - 1.39
S-(1,2-dichlorovinyl)-L-cysteine
0.47
S-(2-aminoethyl)-L-Cys
-
-
0.01 - 1.66
S-(2-benzothiazolyl)-L-cysteine
1.02 - 1.78
S-(2-chloro-1,1,2-trifluoroethyl)-L-cysteine
0.7
S-(p-bromophenyl)-L-cysteine
-
-
0.1 - 0.33
S-1,2-dichlorovinyl-L-cysteine
0.08
S-2,4-dinitrophenyl-L-cysteine
-
-
0.73
S-Benzyl-L-cysteine
-
-
2.3 - 5.3
S-methyl-L-cysteine
2.3
S-methyl-L-cysteine sulfoxide
-
-
4.6
Se-(methyl)-L-selenocysteine
-
-
additional information
additional information
-
0.5
2,4-dinitrophenyl-L-cysteine
-
-
0.5
2,4-dinitrophenyl-L-cysteine
-
-
0.5
2,4-dinitrophenyl-L-cysteine
-
-
0.5
2,4-dinitrophenyl-L-cysteine
-
-
0.5
2,4-dinitrophenyl-L-cysteine
-
-
0.02
cystathionine
recombinant His-tagged enzyme, pH 8.0, 37°C
0.4
cystathionine
recombinant His-tagged enzyme, pH 8.0, 37°C, Tris buffer
0.7
cystathionine
recombinant His-tagged enzyme, pH 8.0, 37°C, bicine buffer
1.3
cystathionine
recombinant His-tagged enzyme, pH 8.0, 70°C, bicine buffer
1.62
cystathionine
-
pH 9.0, 30°C
2.1
cystathionine
recombinant MBP-tagged enzyme, pH 8.0, 37°C
2.4
cystathionine
pH 8.5, 37°C, recombinant enzyme
1
DL-homocysteine
Ctl2, at pH 5.5 and 37°C
1.4
DL-homocysteine
Ctl1, at pH 5.5 and 37°C
0.04
L-cystathionine
-
-
0.068
L-cystathionine
pH 8.5, 25°C, recombinant mutant S32A
0.096
L-cystathionine
in 50 mM phosphate buffer, pH 8.5, 0.02 mM pyridoxal 5'-phosphate, 1 mM EDTA, at 37°C
0.101
L-cystathionine
pH 8.5, 25°C, recombinant mutant W131F
0.104
L-cystathionine
pH 8.5, 25°C, recombinant mutant S33A
0.108
L-cystathionine
pH 8.5, 25°C, recombinant wild-type enzyme
0.116
L-cystathionine
pH 8.5, 25°C, recombinant wild-type enzyme
0.121
L-cystathionine
pH 8.5, 25°C, recombinant mutant W188F
0.131
L-cystathionine
pH 8.5, 25°C, recombinant mutant W276F
0.148
L-cystathionine
pH 8.5, 25°C, recombinant mutant W131F/W188F/W230F/W276F/W300F
0.15
L-cystathionine
-
chloroplastic isoenzyme
0.161
L-cystathionine
pH 8.5, 25°C, recombinant mutant W300F
0.171
L-cystathionine
pH 8.5, 25°C, recombinant mutant W131FF/W230FF/W276F
0.18
L-cystathionine
-
wild type enzyme, at 25°C
0.19
L-cystathionine
pH 8.5, 25°C, recombinant mutant W230F
0.22
L-cystathionine
pH 8.5, 25°C, recombinant mutant K42A
0.27
L-cystathionine
pH 8.5, 25°C, recombinant mutant W131F/W188F/W230F/W276F
0.31
L-cystathionine
pH 8.5, 25°C, recombinant mutant W131F/W230F/W276F/W300F
0.33
L-cystathionine
Ctl2, in sodium phosphate buffer, at pH 5.5 and 37°C
0.35
L-cystathionine
Ctl1, in sodium phosphate buffer, at pH 5.5 and 37°C
0.37
L-cystathionine
Ctl1, in sodium phosphate buffer, at pH 6.8 and 37°C
0.41
L-cystathionine
Ctl2, in sodium phosphate buffer, at pH 6.8 and 37°C
0.55
L-cystathionine
-
mutant enzyme D116N, at 25°C
0.67
L-cystathionine
-
mutant enzyme D116A, at 25°C
0.91
L-cystathionine
pH 8.5, 25°C, recombinant mutant W340F
1
L-cystathionine
pH 8.5, 25°C, recombinant mutant W131F/W230F/W276F/W340F
1.02
L-cystathionine
-
mutant enzyme R59A, at 25°C
1.05
L-cystathionine
pH 8.5, 25°C, recombinant mutant W131F/W188F/W276F/W300F/W340F
1.2
L-cystathionine
-
pH 8.0, 37°C, purified recombinant enzyme
1.26
L-cystathionine
pH 8.5, 25°C, recombinant mutant W131F/W230F/W276F/W300F/W340F
1.3
L-cystathionine
-
pH 8.5, 37°C, purified recombinant enzyme
1.35
L-cystathionine
-
mutant enzyme W340F, at 25°C
1.51
L-cystathionine
-
mutant enzyme R59K, at 25°C
1.8
L-cystathionine
pH 8.5, 25°C, recombinant mutant W131F/W188F/W230F/W276F/W300F/W340F
1.8
L-cystathionine
pH 8.5, 25°C, recombinant mutant W131F/W188F/W230F/W276F/W340F
2
L-cystathionine
pH 8.5, 25°C, recombinant mutant W188F/W230F/W276F/W300F/W340F
2.1
L-cystathionine
pH 8.5, 25°C, recombinant mutant W131F/W188F/W230F/W300F/W340F
3.8
L-cystathionine
pH 8.5, 25°C, recombinant chimeric mutant enzyme eCGS-sC-eCBL
5.1
L-cystathionine
-
mutant enzyme R58A, at 25°C
15
L-cystathionine
-
mutant enzyme R372K, at 25°C
0.29
L-cysteine
-
in presence of 10 mM dithiothreitol
0.3
L-cysteine
Ctl2, at pH 5.5 and 37°C
1
L-cysteine
Ctl1, at pH 5.5 and 37°C
11.5
L-cysteine
pH 10.0, 35°C, recombinant His-tagged enzyme
0.0812
L-cystine
-
-
0.033
L-djenkolate
-
-
0.178
L-djenkolate
in 50 mM phosphate buffer, pH 8.5, 0.02 mM pyridoxal 5'-phosphate, 1 mM EDTA, at 37°C
0.34
L-djenkolate
-
chloroplastic isoenzyme
32.8
L-methionine
Ctl1, at pH 5.5 and 37°C
34.2
L-methionine
Ctl2, at pH 5.5 and 37°C
1.36
S-(1,2-dichlorovinyl)-L-cysteine
-
cytoplasm
1.39
S-(1,2-dichlorovinyl)-L-cysteine
-
mitochondria
0.01
S-(2-benzothiazolyl)-L-cysteine
-
-
0.29
S-(2-benzothiazolyl)-L-cysteine
-
-
0.66
S-(2-benzothiazolyl)-L-cysteine
-
mitochondria
1.66
S-(2-benzothiazolyl)-L-cysteine
-
cytoplasm
1.02
S-(2-chloro-1,1,2-trifluoroethyl)-L-cysteine
-
mitochondria
1.78
S-(2-chloro-1,1,2-trifluoroethyl)-L-cysteine
-
cytoplasm
0.1
S-1,2-dichlorovinyl-L-cysteine
-
-
0.33
S-1,2-dichlorovinyl-L-cysteine
-
-
2.3
S-methyl-L-cysteine
-
-
5.1
S-methyl-L-cysteine
-
-
5.3
S-methyl-L-cysteine
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
steady-state kinetics
-
additional information
additional information
-
kinetic parameters for differentially substituted cysteine-S-conjugates and selenocysteine Se-conjugates
-
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
-
kinetics, overview
-
additional information
additional information
Michaelis-Menten steady-state kinetics
-
additional information
additional information
Michaelis-Menten steady-state kinetics
-
additional information
additional information
Michaelis-Menten steady-state kinetics
-