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1.25
1-amino-3-(methylthio)propylphosphinic acid
-
30°C
18.2
2-amino-3-(N,N-dimethylamino)propionic acid
-
-
12.3
DL-2-amino-3(N-hydroxyethyl)propionic acid
-
-
2.3
DL-amino-3-(N-methylamino)propionic acid
-
-
1.2
L-1-amino-3-methylthiopropylphosphinic acid
pH and temperature not specified in the publication
6.7
L-Gly(vinyl)
-
30°C, pH 8.0
1.78 - 6.43
L-homocysteine
4 - 38
L-methionine sulfone
6.2 - 33.51
L-methionine sulfoxide
2.3
L-selenodjenkolate
-
-
6.7 - 7.22
L-Vinylglycine
5.82
O-acetyl-DL-homoserine
-
pH 8.0, 30°C
2.22 - 6
O-acetyl-L-homoserine
2.88 - 52.33
O-acetyl-L-serine
2.8
O-methyl-L-serine
-
-
0.18 - 2.75
S-Benzyl-L-cysteine
0.17 - 8.19
S-ethyl-L-cysteine
0.278 - 8.11
S-ethyl-L-homocysteine
0.4 - 2.62
S-methyl-L-cysteine
0.77
S-methylcysteine
-
-
0.13
Se-methyl-L-selenocysteine
-
-
0.1 - 5.45
trifluoromethionine
additional information
L-methionine
0.44
alliin
mutant C115H, pH 8.0, 30°C
1.75
alliin
mutant C115A, pH 8.0, 30°C
3.6
cysteine
-
recombinant, MGL2 C116G
8.5
cysteine
-
recombinant MGL1
9.7
cysteine
-
recombinant MGL1, C113G
22.3
cysteine
-
recombinant MGL2
0.2
DL-homocysteine
pH 8.0, 37°C, recombinant mutant Y114F/C116H
0.24
DL-homocysteine
mutant enzyme C116S, at 37°C
0.6
DL-homocysteine
pH 8.0, 37°C, recombinant mutant K240R
0.8
DL-homocysteine
pH 8.0, 37°C, recombinant mutant K240N
0.97
DL-homocysteine
recombinant enzyme
0.97
DL-homocysteine
-
pH 8.0, 30°C
1
DL-homocysteine
-
30°C, pH 8.0
1.1
DL-homocysteine
wild-type
1.1
DL-homocysteine
pH 8.0, 37°C, recombinant wild-type enzyme
1.4
DL-homocysteine
pH 8.0, 37°C, recombinant mutant C166H
1.4
DL-homocysteine
pH 8.0, 37°C, recombinant mutant K240M
1.47
DL-homocysteine
wild-type ehMGL2, pH 7.0, 37°C
1.61
DL-homocysteine
mutant enzyme C116A, at 37°C
1.8
DL-homocysteine
mutant enzyme C116H, at 37°C
1.82
DL-homocysteine
wild type enzyme, at 37°C
1.87
DL-homocysteine
36°C, pH 6.8, recombinant EhMGL2
3.03
DL-homocysteine
wild-type ehMGL1, pH 7.0, 37°C
3.4
DL-homocysteine
36°C, pH 6.8, recombinant EhMGL1
3.46
DL-homocysteine
mutant Y58F, pH 8.0, 30°C
6
DL-homocysteine
pH 8.0, 37°C, recombinant mutant K240I
56.5
DL-homoserine
-
pH 8.0, 30°C
56.5
DL-homoserine
-
30°C, pH 8.0
0.457
ethiin
mutant C115H, pH 8.0, 30°C
5.48
ethiin
mutant C115A, pH 8.0, 30°C
0.12
L-cysteine
mutant enzyme C116S, at 37°C
0.16
L-cysteine
recombinant enzyme
0.16
L-cysteine
-
30°C, pH 8.0
0.18
L-cysteine
wild-type
0.18
L-cysteine
pH 7.6, 37°C
0.2
L-cysteine
pH 8.0, 37°C
0.2
L-cysteine
pH 8.0, 37°C, recombinant wild-type enzyme
0.2
L-cysteine
pH 8.0, 37°C, recombinant mutant Y114F/C116H
0.3
L-cysteine
pH 8.0, 37°C, recombinant mutant C166H
0.3
L-cysteine
pH 8.0, 37°C, recombinant mutants K240R and K240M
0.32
L-cysteine
pH 7.6, 37°C
0.34
L-cysteine
mutant C110G, pH 7.0, 37°C
0.39
L-cysteine
mutant enzyme C116A, at 37°C
0.46
L-cysteine
mutant C110S, pH 7.0, 37°C
0.53
L-cysteine
wild type enzyme, at 37°C
0.63
L-cysteine
pH 7.6, 37°C
0.63
L-cysteine
mutant enzyme C116H, at 37°C
0.64
L-cysteine
wild-type ehMGL1, pH 7.0, 37°C
1
L-cysteine
pH 8.0, 37°C, recombinant mutants K240I and K240N
1.01
L-cysteine
mutant Y108F, pH 7.0, 37°C
1.7
L-cysteine
wild-type ehMGL2, pH 7.0, 37°C
0.2
L-ethionine
-
-
0.2
L-ethionine
-
37°C, pH 8.0
0.27
L-ethionine
-
37°C, pH 8.0
0.54
L-ethionine
recombinant enzyme
0.56
L-ethionine
wild-type
6.2
L-ethionine
-
recombinant MGL2, C116G
12.2
L-ethionine
-
recombinant MGL1
15.2
L-ethionine
-
recombinant MGL1, C113G
37.2
L-ethionine
-
recombinant MGL2
1.78
L-homocysteine
pH 8.0, 37°C
30
L-homoserine
-
-
30
L-homoserine
-
37°C, pH 8.0
0.0013
L-methionine
-
-
0.04
L-methionine
pH 8.0, 37°C, recombinant mutant K240R
0.11
L-methionine
mutant enzyme C116S, at 37°C
0.19
L-methionine
mutant C110G, pH 7.0, 37°C
0.3
L-methionine
mutant C115A, pH 8.0, 30°C
0.432
L-methionine
-
pH 8.0, 30°C, recombinant enzyme
0.5
L-methionine
pH 8.0, 37°C, recombinant wild-type enzyme
0.61
L-methionine
wild-type ehMGL1, pH 7.0, 37°C
0.7
L-methionine
wild-type
0.7
L-methionine
recombinant enzyme
0.7
L-methionine
-
pH 8.0, 30°C
0.7
L-methionine
-
30°C, pH 8.0
0.72
L-methionine
mutant C110S, pH 7.0, 37°C
0.8
L-methionine
wild-type, pH 7.2, 30°C
0.9
L-methionine
-
37°C, pH 8.0
0.92
L-methionine
wild type enzyme, at 37°C
0.94
L-methionine
36°C, pH 6.8, recombinant EhMGL1
0.947
L-methionine
-
pH 8.0, 30°C, recombinant enzyme
1.2
L-methionine
pH 7.5, 37°C
1.42
L-methionine
mutant enzyme C116H, at 37°C
1.6
L-methionine
pH 8.0, 37°C
1.6
L-methionine
mutant P360Q, pH 7.2, 30°C
1.7
L-methionine
-
37°C, pH 8.0
1.77
L-methionine
-
pH 8.0, 30°C, recombinant enzyme
1.9
L-methionine
36°C, pH 6.8, recombinant EhMGL2
2.3
L-methionine
pH 8.0, 37°C, recombinant mutant C166H
2.7
L-methionine
mutant V358Y, pH 7.2, 30°C
2.85
L-methionine
mutant enzyme C116A, at 37°C
3.58
L-methionine
wild-type ehMGL2, pH 7.0, 37°C
3.7
L-methionine
mutant P357I, pH 7.2, 30°C
4.8
L-methionine
pH 8.0, 37°C, recombinant mutant K240I
5.5
L-methionine
mutant A366Y, pH 7.2, 30°C
5.7
L-methionine
pH 8.0, 37°C, recombinant mutant K240M
9.8
L-methionine
pH 8.0, 37°C, recombinant mutant K240N
10.6
L-methionine
-
pH 7.2, 30°C
15.12
L-methionine
mutant C113S, pH 7.0, 37°C
19.65
L-methionine
mutant Y58F, pH 8.0, 30°C
98
L-methionine
pH 8.0, 23°C
4
L-methionine sulfone
-
pH 8.0, 30°C
8.22
L-methionine sulfone
-
37°C, pH 8.0
38
L-methionine sulfone
pH 7.5, 37°C
6.2
L-methionine sulfoxide
-
pH 8.0, 30°C
7.07
L-methionine sulfoxide
-
pH 8.0, 30°C, recombinant enzyme
12.22
L-methionine sulfoxide
-
pH 8.0, 30°C, recombinant enzyme
33.51
L-methionine sulfoxide
-
pH 8.0, 30°C, recombinant enzyme
6.7
L-Vinylglycine
-
pH 8.0, 30°C
7.22
L-Vinylglycine
-
37°C, pH 8.0
0.57
methiin
mutant C115H, pH 8.0, 30°C
0.63
methiin
mutant C115A, pH 8.0, 30°C
2.22
O-acetyl-L-homoserine
-
37°C, pH 8.0
2.46
O-acetyl-L-homoserine
mutant C115H, pH 8.0, 30°C
6
O-acetyl-L-homoserine
mutant C115A, pH 8.0, 30°C
2.88
O-acetyl-L-serine
mutant C115A, pH 8.0, 30°C
3.68
O-acetyl-L-serine
mutant C115H, pH 8.0, 30°C
6.28
O-acetyl-L-serine
wild-type ehMGL1, pH 7.0, 37°C
52.33
O-acetyl-L-serine
wild-type ehMGL2, pH 7.0, 37°C
0.18
S-Benzyl-L-cysteine
recombinant enzyme
0.18
S-Benzyl-L-cysteine
-
30°C, pH 8.0
0.19
S-Benzyl-L-cysteine
wild-type
0.348
S-Benzyl-L-cysteine
-
pH 8.0, 30°C, recombinant enzyme
0.766
S-Benzyl-L-cysteine
-
pH 8.0, 30°C, recombinant enzyme
1.47
S-Benzyl-L-cysteine
-
pH 8.0, 30°C, recombinant enzyme
2.75
S-Benzyl-L-cysteine
mutant Y58F, pH 8.0, 30°C
0.17
S-ethyl-L-cysteine
recombinant enzyme
0.17
S-ethyl-L-cysteine
-
30°C, pH 8.0
0.17
S-ethyl-L-cysteine
pH and temperature not specified in the publication
0.26
S-ethyl-L-cysteine
mutant C115A, pH 8.0, 30°C
0.358
S-ethyl-L-cysteine
-
pH 8.0, 30°C, recombinant enzyme
0.48
S-ethyl-L-cysteine
-
37°C, pH 8.0
0.49
S-ethyl-L-cysteine
wild-type
0.72
S-ethyl-L-cysteine
-
pH 8.0, 30°C, recombinant enzyme
0.93
S-ethyl-L-cysteine
mutant C115H, pH 8.0, 30°C
2.17
S-ethyl-L-cysteine
-
pH 8.0, 30°C, recombinant enzyme
8.19
S-ethyl-L-cysteine
mutant Y58F, pH 8.0, 30°C
0.278
S-ethyl-L-homocysteine
-
pH 8.0, 30°C, recombinant enzyme
0.5
S-ethyl-L-homocysteine
-
30°C, pH 8.0
0.545
S-ethyl-L-homocysteine
-
pH 8.0, 30°C, recombinant enzyme
0.93
S-ethyl-L-homocysteine
-
pH 8.0, 30°C, recombinant enzyme
8.11
S-ethyl-L-homocysteine
mutant Y58F, pH 8.0, 30°C
0.4
S-methyl-L-cysteine
-
37°C, pH 8.0
0.4
S-methyl-L-cysteine
pH 8.0, 37°C, recombinant mutant Y114F/C116H
0.61
S-methyl-L-cysteine
-
-
0.61
S-methyl-L-cysteine
wild-type
0.69
S-methyl-L-cysteine
-
-
0.7
S-methyl-L-cysteine
pH 8.0, 37°C, recombinant wild-type enzyme
0.71
S-methyl-L-cysteine
recombinant enzyme
0.71
S-methyl-L-cysteine
-
30°C, pH 8.0
0.77
S-methyl-L-cysteine
-
-
0.8
S-methyl-L-cysteine
mutant C115A, pH 8.0, 30°C
1.4
S-methyl-L-cysteine
-
-
2.1
S-methyl-L-cysteine
pH 8.0, 37°C, recombinant mutant C166H
2.62
S-methyl-L-cysteine
mutant C115H, pH 8.0, 30°C
0.1
trifluoromethionine
-
-
0.1
trifluoromethionine
wild-type ehMGL1, pH 7.0, 37°C
0.29
trifluoromethionine
mutant Y111F, pH 7.0, 37°C
0.57
trifluoromethionine
mutant Y108F, pH 7.0, 37°C
0.83
trifluoromethionine
mutant R55A, pH 7.0, 37°C
0.92
trifluoromethionine
wild-type ehMGL2, pH 7.0, 37°C
1.62
trifluoromethionine
mutant R58A, pH 7.0, 37°C
5.45
trifluoromethionine
mutant C113S, pH 7.0, 37°C
additional information
L-methionine
-
values between 0.8 to 1.7, depending on publications
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
values for native and cyanylated enzyme
-
additional information
additional information
steady-state kinetics
-
additional information
additional information
-
steady-state kinetics for beta- and gamma-elimination reactions, overview
-
additional information
additional information
-
steady-state kinetics for beta- and gamma-elimination reactions, overview
-
additional information
additional information
-
steady-state kinetics for beta- and gamma-elimination reactions, overview
-
additional information
additional information
-
steady-state kinetics for beta- and gamma-elimination reactions, overview
-
4.4.1.11 methionine gamma-lyase
results (
results (
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