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4.3.2.2: adenylosuccinate lyase

This is an abbreviated version!
For detailed information about adenylosuccinate lyase, go to the full flat file.

Word Map on EC 4.3.2.2

Reaction

(S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate
=
fumarate
 +
5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide

Synonyms

adenylosuccinase, adenylosuccinate lyase, ADL, ADSL, AMPS lyase, ASASE, ASL, Glutamyl-tRNA synthetase regulatory factor, lyase, adenylosuccinate, PurB, succino AMP-lyase, succino-AMP lyase

ECTree

     4 Lyases
         4.3 Carbon-nitrogen lyases
             4.3.2 Amidine-lyases
                4.3.2.2 adenylosuccinate lyase

Storage Stability

Storage Stability on EC 4.3.2.2 - adenylosuccinate lyase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C or -80°C, pH 7.0, 150 mM KCl, 1 mM EDTA, 1 mM DTT and 10% glycerol, 3 months, the recombinant enzyme loses more than 50% of initial activity
-70°C, 10% glycerol, 1 mM dithiothreitol, 0.01 M Tris, pH 7.4, activity in lysates from all cell lines is stable for more than 6 weeks
-
-70°C, unstable to storage
-
-80°C or -20°C, recombinant enzyme is very unstable regardless of salt or glycerol concentration
-
-80°C, potassium phosphate buffer, KCl, DTT, EDTA, glycerol
-
2°C or -20°C, stable for two months
-
4°C, stable for several weeks
-