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4.3.2.2: adenylosuccinate lyase

This is an abbreviated version!
For detailed information about adenylosuccinate lyase, go to the full flat file.

Word Map on EC 4.3.2.2

Reaction

(S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate
=
fumarate
 +
5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide

Synonyms

adenylosuccinase, adenylosuccinate lyase, ADL, ADSL, AMPS lyase, ASASE, ASL, Glutamyl-tRNA synthetase regulatory factor, lyase, adenylosuccinate, PurB, succino AMP-lyase, succino-AMP lyase

ECTree

     4 Lyases
         4.3 Carbon-nitrogen lyases
             4.3.2 Amidine-lyases
                4.3.2.2 adenylosuccinate lyase

Specific Activity

Specific Activity on EC 4.3.2.2 - adenylosuccinate lyase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000083
-
mutant R310Q, pH 7.0
0.004
mutant S295A, pH 7.0
0.008
mutant H171N, pH 7.0
0.009
mutant H171A, pH 7.0
0.031
mutant H171A, pH 8.5
0.063
-
mutant I62D/D65R, adenylsuccinate, 50 mM HEPES, pH 7.0, 25°C
0.068
-
complementation of inactive mutant ASLs, maximum specific activity, T93A + H141Q, 3.0% wild-type activity
0.069
-
mutant I62D/D65R, succinylaminoimidazole carboxamide, 50 mM HEPES, pH 7.0, 25°C
0.072
-
mutant I62D/D65R/D69E, adenylsuccinate, 50 mM HEPES, pH 7.0, 25°C
0.094
-
mutant I62D/D65R/D69E, succinylaminoimidazole carboxamide, 50 mM HEPES, pH 7.0, 25°C
0.096
-
complementation of inactive mutant ASLs, maximum specific activity, S306A + H141Q, 4.6% wild-type activity
0.1
-
mutant R310K, pH 7.0
0.18
-
mutant I62D/D65R + I62E/D65R/D69E, adenylsuccinate, 50 mM HEPES, pH 7.0, 25°C
0.2
wild-type enzyme at 4 mg/ml and 4°C
0.21
-
complementation of inactive mutant ASLs, maximum specific activity, T93A + K268Q, 10.1% wild-type activity
0.33
-
mutant I62E/D65R/D69E, adenylsuccinate, 50 mM HEPES, pH 7.0, 25°C
0.46
-
mutant I62E/D65R/D69E, succinylaminoimidazole carboxamide, 50 mM HEPES, pH 7.0, 25°C
0.53
-
mutant I62E/D65R/D69E, succinylaminoimidazole carboxamide, 50 mM HEPES, pH 7.0, 25°C
0.54
-
mutant I62E/D65R, adenylsuccinate, 50 mM HEPES, pH 7.0, 25°C
0.97
-
mutant I62E/D65R/D69E, adenylsuccinate + succinylaminoimidazole carboxamide, 50 mM HEPES, pH 7.0, 25°C
1
-
mutant R396H, pH 7.4, 25°C
1.07
-
adenylsuccinate and succinylaminoimidazole carboxamide, 50 mM HEPES, pH 7.0, 25°C
1.09
-
mutant I62D/D65R, adenylsuccinate + succinylaminoimidazole carboxamide, 50 mM HEPES, pH 7.0, 25°C
1.1
-
mutant R396H, after treatment with guanidiniumHCl, pH 7.4, 25°C
1.29
-
mutant I62D/D65R + I62E/D65R/D69E, adenylsuccinate + succinylaminoimidazole carboxamide, 50 mM HEPES, pH 7.0, 25°C
1.3
-
mutant I62D/D65R/D69E, adenylsuccinate + succinylaminoimidazole carboxamide, 50 mM HEPES, pH 7.0, 25°C
1.4
-
mutant I62E/D65R/D69E, adenylsuccinate + succinylaminoimidazole carboxamide, 50 mM HEPES, pH 7.0, 25°C
1.58
-
adenylsuccinate, 50 mM HEPES, pH 7.0, 25°C
1.6
-
mutant R396C, after treatment with guanidiniumHCl, pH 7.4, 25°C
1.75
-
wild-type enzyme, pH 7.0
1.8
-
mutant R396C, pH 7.4, 25°C
13.9
-
purified recombinant His-tagged wild-type enzyme, substrate succinyladenosine monophosphate, pH 7.4, 25°C
16.47
wild-type protein, pH 8.5
2.4
-
-
20
-
crude cell extract, presence of 1 M KCl, pH 7.5, temperature not specified in the publication
23.3
-
purified recombinant His-tagged wild-type enzyme, substrate phosphoribosylsuccinyl-aminoimidazole carboxamide, pH 7.4, 25°C
3.8
-
non tagged enzyme
4.65
wild-type protein, pH 7.0
40
-
crude cell extract, presence of 1 M KCl, pH 7.5, temperature not specified in the publication
6.4
-
purified recombinant His-tagged mutant R426H enzyme, substrate phosphoribosylsuccinyl-aminoimidazole carboxamide, pH 7.4, 25°C
6.7
-
purified recombinant His-tagged mutant R426H enzyme, substrate succinyladenosine monophosphate, pH 7.4, 25°C
7.5
-
His-tagged mutant L311V, after treatment with guanidiniumHCl, pH 7.4, 25°C
7.8
-
His-tagged mutant L311V, pH 7.4, 25°C
8.3
-
His-tagged wild-type, after treatment with guanidiniumHCl, pH 7.4, 25°C
8.4
-
wild-type, after treatment with guanidiniumHCl, pH 7.4, 25°C
8.5
-
His-tagged wild-type, pH 7.4, 25°C
8.6
-
wild-type, pH 7.4, 25°C
8.7
-
His-tagged mutant R194C, pH 7.4, 25°C
8.9
-
His-tagged mutant R194C, after treatment with guanidiniumHCl, pH 7.4, 25°C
additional information