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4.3.2.2: adenylosuccinate lyase

This is an abbreviated version!
For detailed information about adenylosuccinate lyase, go to the full flat file.

Word Map on EC 4.3.2.2

Reaction

(S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate
=
fumarate
 +
5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide

Synonyms

adenylosuccinase, adenylosuccinate lyase, ADL, ADSL, AMPS lyase, ASASE, ASL, Glutamyl-tRNA synthetase regulatory factor, lyase, adenylosuccinate, PurB, succino AMP-lyase, succino-AMP lyase

ECTree

     4 Lyases
         4.3 Carbon-nitrogen lyases
             4.3.2 Amidine-lyases
                4.3.2.2 adenylosuccinate lyase

Purification

Purification on EC 4.3.2.2 - adenylosuccinate lyase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-affinity chromatography
-
Ni-NTA agarose column chromatography
normal and mutant enzyme S413P
-
Q-Sepharose column chromatography and gel filtration
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain Rosetta 2(DE3)pLysS by nickel affinity chromatography
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain Rosetta 2(DE3)pLysS by nickel affinity chromatography, dialysis, cleavage of the His-tag through thrombin, and gel filtration, to homogeneity
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain Bl21 by nickel affinity chromatography
using a Ni-NTA column
using a Ni-NTA column, the 6-histidine tag is removed by cleavage with thrombin, separation of thrombin and histidine-tag from the enzyme is achieved by using a Sephadex G-100 column
-
using nickel affinity chromatography
-
using nickel nitrilotriacetic acid-agarose
-