4.3.2.2: adenylosuccinate lyase

This is an abbreviated version!
For detailed information about adenylosuccinate lyase, go to the full flat file.

Reaction

Synonyms

adenylosuccinase, adenylosuccinate lyase, ADL, ADSL, AMPS lyase, ASASE, ASL, Glutamyl-tRNA synthetase regulatory factor, lyase, adenylosuccinate, PurB, succino AMP-lyase, succino-AMP lyase

ECTree

     4 Lyases

         4.3 Carbon-nitrogen lyases

             4.3.2 Amidine-lyases

                4.3.2.2 adenylosuccinate lyase

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Results	in table
44124	AA Sequence
8	Application
1	CAS Registry Number
22	Cloned(Commentary)
13	Crystallization (Commentary)
462	Disease/ Diagnostics
1	Expression
24	General Information
6	General Stability
74	Inhibitors
9	kcat/KM [mM/s]
3	Ki Value [mM]
72	KM Value [mM]
2	Localization
3	Metals/Ions
72	Molecular Weight [Da]
88	Natural Substrates/ Products (Substrates)
69	Organism
11	Pathway
82	PDB ID
21	pH Optimum
3	pH Range
102	Protein Variants
19	Purification (Commentary)
9	Reaction
10	Reaction Type
69	Reference
29	Source Tissue
69	Specific Activity [micromol/min/mg]
7	Storage Stability
145	Substrates and Products (Substrate)
34	Subunits
52	Synonyms
1	Systematic Name
12	Temperature Optimum [°C]
5	Temperature Stability [°C]
12	Turnover Number [1/s]

General Stability

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General Stability on EC 4.3.2.2 - adenylosuccinate lyase

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GENERAL STABILITY

ORGANISM

UNIPROT

LITERATURE

dialysis for 24 h at 5°C, activity in mutant lysates is decreased by 95% while 50-60% of the initial activity remains in the normal cell lysates

Homo sapiens

-

34462

freezing at -80°C, followed by thawing, 20-40% loss of enzyme activity in extract

Homo sapiens

-

34466

stable to thawing and refreezing at a protein concentration of above 2 mg/ml

Rattus norvegicus

-

34449

the structure of adenylosuccinate lyase reveals that this protein contains three disulfide bonds that contribute significantly to its stability against thermal and chemical denaturation

Pyrobaculum aerophilum

Q8ZY28

728121

three cycles of freezing and thawing remove nearly all enzyme activity

Rattus norvegicus

-

34456

unstable to quick freezing in dry ice/acetone

Leishmania donovani

-

1541