4.2.3.4: 3-dehydroquinate synthase

This is an abbreviated version!
For detailed information about 3-dehydroquinate synthase, go to the full flat file.

Word Map on EC 4.2.3.4

4.2.3.4

shikimate

dahp

3-deoxy-d-arabino-heptulosonate

chorismate

pentafunctional

3-phosphoshikimate

3-dehydroquinase

1-carboxyvinyltransferase

drug development

medicine

Reaction

3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate

Synonyms

3-dehydroquinate synthase, 3-dehydroquinate synthetase, 3-dehydroquinic acid synthetase, 5-dehydroquinate synthase, 5-dehydroquinic acid synthetase, aroB, dehydroquinate synthase, dehydroquinate synthetase, DHQ synthase, DHQS, EC 4.6.1.3, hpDHQS, synthase, 5-dehydroquinate, Xoo1243

ECTree

4 Lyases

4.2 Carbon-oxygen lyases

4.2.3 Acting on phosphates

4.2.3.4 3-dehydroquinate synthase

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Results	in table
1	Activating Compound
35355	AA Sequence
4	Application
1	CAS Registry Number
20	Cloned(Commentary)
26	Cofactor
10	Crystallization (Commentary)
1	General Information
1	General Stability
3	IC50 Value
65	Inhibitors
11	kcat/KM [mM/s]
3	Ki Value [mM]
27	KM Value [mM]
1	Localization
35	Metals/Ions
47	Molecular Weight [Da]
14	Natural Substrates/ Products (Substrates)
81	Organism
6	Pathway
69	PDB ID
6	pH Optimum
2	pH Range
17	Purification (Commentary)
3	Reaction
10	Reaction Type
69	Reference
6	Source Tissue
34	Specific Activity [micromol/min/mg]
6	Storage Stability
42	Substrates and Products (Substrate)
15	Subunits
43	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
1	Temperature Range [°C]
6	Temperature Stability [°C]
15	Turnover Number [1/s]

Purification

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Purification on EC 4.2.3.4 - 3-dehydroquinate synthase

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5 entries

arom-multienzyme complex

Neurospora crassa

137628, 137638, 137639

by Ni2+-NTA affinity chromatography, enzyme is subjected to a TEV protease cleavage reaction to cleave the six His-tag residues at the N-terminus, further purification by ion-exchange chromatography

Xanthomonas oryzae

690254

expressed HpDHQS protein is purified by immobilized nickel-ion chromatography

partial

together with chorismate synthase

Bacillus subtilis

137636

using a Q-Sepharose Fast Flow, a HiLoad 16/10 phenyl-Sepharose, and a Sephacryl S-200 HR column

Mycobacterium tuberculosis

P9WPX9

680499