4.2.3.15: myrcene synthase

This is an abbreviated version!
For detailed information about myrcene synthase, go to the full flat file.

Word Map on EC 4.2.3.15

4.2.3.15

terpene

synthases

monoterpene

sesquiterpene

herbivore

limonene

emit

lepidopteran

enemies

gymnosperm

herbivore-induced

scent

jasmonate-induced

parasitoids

olfactometer

cotesia

e-beta-ocimene

carvone

carbocation

e-beta-farnesene

tepals

neryl

pinene

Reaction

Synonyms

AaTPS2, AcTPS2, AtTPS10, EC 4.1.99.9, myrcene synthase, myrcene/(E)-beta-ocimene synthase, TPS10, TPS2

ECTree

4 Lyases

4.2 Carbon-oxygen lyases

4.2.3 Acting on phosphates

4.2.3.15 myrcene synthase

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Results	in table
45	AA Sequence
1	CAS Registry Number
8	Cloned(Commentary)
2	Expression
2	General Information
1	kcat/KM [mM/s]
2	KM Value [mM]
10	Metals/Ions
5	Molecular Weight [Da]
7	Natural Substrates/ Products (Substrates)
10	Organism
5	Pathway
3	pH Optimum
1	pI Value
3	Protein Variants
2	Purification (Commentary)
1	Reaction
1	Reaction Type
10	Reference
9	Source Tissue
21	Substrates and Products (Substrate)
2	Subunits
8	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
1	Turnover Number [1/s]

Cloned

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Cloned on EC 4.2.3.15 - myrcene synthase

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Alstroemeria caryophyllaea

730124

expressed in Escherichia coli

2 entries

expression in Escherichia coli

3 entries

gene TPS2, cloning from a cDNA library, sequence comparisons and phylogenetic analysis and tree, quantitative RT-PCR expression analysis, recombinant expression of His-tagged enzyme without after removing the N-terminal plastid targeting sequence in Escherichia coli strain BL21(DE3)

Artemisia annua

A0A068L9J0

747873

mutated enzymes are generated using the QuickChange II site-directed mutagenesis kit. The PCR-based mutagenesis protocol is performed using pET200-D-TOPO harbouring the respective AcTPS2 cDNAs as template. The single-site mutant enzymes overexpressed in Escherichia coli.

Actinidia chinensis

693257