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4.2.1.96: 4a-hydroxytetrahydrobiopterin dehydratase

This is an abbreviated version!
For detailed information about 4a-hydroxytetrahydrobiopterin dehydratase, go to the full flat file.

Word Map on EC 4.2.1.96

Reaction

4a-hydroxytetrahydrobiopterin
=
6,7-dihydrobiopterin
 +
H2O

Synonyms

4 alpha-Hydroxy-tetrahydropterin dehydratase, 4-alpha-hydroxy-tetrahydropterin dehydratase, 4-alpha-hydroxytetrahydrobiopterin dehydratase, 4a-Carbinolamine dehydratase, 4a-Hydroxytetrahydrobiopterin dehydratase, 4a-Hydroxytetrahydropterin dehydratase, carbinolamine-4a-dehydratase, cDcoH, DCoH, DCoH/PCD, DcoH2, DCoHalpha, Dehydratase, 4a-carbinolamine, Dimerization cofactor of hepatocyte nuclear factor 1-alpha, Dimerization cofactor of HNF1, Dimerization factor of HNF1/pterin-4alpha-carbinolamine dehydratase, GenBank AE000671-derived protein GI 2982796, More, P4aCD, PCD, PCD/DCoH, PCD/PhhB, PCDH, Phenylalanine hydroxylase-stimulating protein, Phenylalanine hydroxylase-stimulating protein/pterin-4alpha-carbinolamine dehydratase, PhhB, PHS, PHS/PCD, pterin 4a-carbinolamine dehydratase, Pterin carbinolamine dehydratase, Pterin-4 alpha-carbinolamine dehydratase, Pterin-4-alpha-carbinolamine dehydratase, Pterin-4a-carbinolamine dehydratase, Pterin-4a-carbinolamine dehydratase (Aquifex aeolicus gene phhB), Pterin-4a-carbinolamine dehydratase/dimerization cofactor of HNF1, Pterin-4alpha-carbinolamine dehydratase, Pterin-4alpha-carbinolamine dehydratase (PCD)/dimerization cofactor for the transcription factor HBF-1alpha, XDCoH

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.96 4a-hydroxytetrahydrobiopterin dehydratase

Cloned

Cloned on EC 4.2.1.96 - 4a-hydroxytetrahydrobiopterin dehydratase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli as GST-tagged enzyme
-
expression in Escherichia coli BL21 (DE3). There is a high degree of structural similarity between Toxoplasma gondii PCD and the mammalian orthologue, Rattus norvegicus PCD, which is identical to the human enzyme
expression in Escherichia coli BL21(DE3)pLysS
expression in recombinant Escherichia coli strain BL21(DE3) tnaA-, co-expression with Chromobacterium violaceum L-phenylalanine 4-hydroxylase mutant L101Y-W180F, Escherichia coli dihydropteridine reductase, and Bacillus subtilis glucose dehydrogenase genes for installation of 5-hydroxy-L-tryptophan biosynthesis in the recombinant Escherichia coli strain, method optimization and development of a cofactor regeneration system for L-tryptophan hydroxylation for enhanced synthesis of 5-hydroxy-L-tryptophan
gene 100277322, the raf2 locus corresponds to GRMZM2G139123, an intron-less locus on chromosome 1
overexpression in Escherichia coli
-
PCD sequence comparisons and phylogenetic tree, enzyme expression analysis
-
plasmids pJS11, which carries phhA, and pBluescript, carrying phhB transformed simultaneously by electroporation into Escherichia coli strains JP2255, JP2255 folM, and JP2255 folX
-
targeting of full-length GFP-tagged PCD sequence to chloroplasts in Arabidopsis thaliana mesophyll protoplasts
the 18 amino acid-truncated mutant E87T cannot be overexpressed in Escherichia coli
-
wild type and mutant enzyme C82R overexpressed in Escherichia coli
-
wild-type and mutant enzymes
-