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4.2.1.79: 2-methylcitrate dehydratase

This is an abbreviated version!
For detailed information about 2-methylcitrate dehydratase, go to the full flat file.

Word Map on EC 4.2.1.79

Reaction

(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate
=
(Z)-but-2-ene-1,2,3-tricarboxylate
 +
H2O

Synonyms

2-MC dehydratase, 2-methylcitrate dehydratase, 2-methylcitrate dehydratase 1, 2-Methylcitrate hydro-lyase, Dehydratase, 2-methylcitrate, methylcitrate dehydratase, mmgE, PrpD, SePrpD, STM0370

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.79 2-methylcitrate dehydratase

Cloned

Cloned on EC 4.2.1.79 - 2-methylcitrate dehydratase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, genomic organization, expression of AcnC in AcnABnull strain, overexpression of PrpD in AcnABnull strain
-
expression in Escherichia coli BL21(DE3)
expression of His-tagged AcnD and PrpF in Escherichia coli BL21(DE3), functional complementation of an enzyme-deficient mutant of Salmonella enterica only by expression of both AcnD and PrpF
-
gene mmgE, the mother cell metabolic gene (mmg) operon encodes homologues from the methylcitric acid cycle
gene prpD in the operon prpBCDE, DNA sequence determination nand analysis, overexpression as N-terminally His-tagged protein in Escherichia coli BL21(DE3)
gene prpD, the mother cell metabolic gene (mmg) operon encodes homologues from the methylcitric acid cycle
gene prpD, the sequence of prp genes is prpR-prpB-prpC-prpD-prpE, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
genomic structure of prp operon, expression studies in Escherichia coli K12 strain W3350, and overexpression of the N-terminally His-tagged enzyme
structural genetic organization of prp operon
-