4.2.1.51: prephenate dehydratase

This is an abbreviated version!
For detailed information about prephenate dehydratase, go to the full flat file.

Word Map on EC 4.2.1.51

Reaction

prephenate
=
phenylpyruvate
+
H2O
+
CO2

Synonyms

Chorismate mutase/prephenate dehydratase, dehydratase, prephenate, monofunctional prephenate dehydratase, P-protein, cyclohexydienyl dehydratase, CM-PD, PDT, P-protein dehydratase, PheA, prephenate dehydratase, MjPDT, Sa-PDT, Ct-PDT, prephenate dehydratase 1, ADT1, ADT2, ADT6, chorismate mutase-prephenate dehydratase, PDT protein, MtbPDT, chorismate mutase prephenate dehydratase, CM–PDT, AroQ, CM/PDT/PDHG, PpADT-B, PpADT-C, PpADT-G, Cmut1, Gmut9, Gmut11

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.51 prephenate dehydratase

Cloned

Cloned on EC 4.2.1.51 - prephenate dehydratase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA sequence determination and analysis of the two-domain aroQ/pheA gene, constitutive expression due to the absence of an attenuator region, changes in the ESRP sequence leading to desensitization to inhibition by phenylalanine
expressed in Corynebacterium glutamicum
expressed in different engineered Escherichia coli WSH-Z06 strains (pAP-B, pAP-B01, pAP-B02 and pAP-B03)
expressed in Escherichia coli
-
expressed in Escherichia coli (DE3)-RIL, pIS85 expression plasmid
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3), pET-23a(+) expression vector
expressed in Escherichia coli, mtr1-D mutant gene, callus transformation by Agrobacterium tumefaciens
expressed in Escherichia coli, PB12 strain, pCR-BluntII-TOPO, additional strains and plasmids listed
expression of mutant enzymes in enzyme deficient strain NK6024, subcloning in strain DH5alpha, overexpression of the isolated His-tagged wild-type prephenate dehydratase domain in strain BL21(DE3)
-
expression of the His-tagged wild-type and mutant enzymes in Escherichia coli JM109
-
gene pheA, overproduced in Escherichia coli
-
generation of transgenic Arabidopsis plants expressing a bacterial feedback-insensitive chorismate mutase/prephenate dehydratase gene (truncated PheA) under the control of the 35S CaMV promoter, fused inframe at the 3' end of the coding sequence to DNA encoding a hemagglutinin epitope tag. Two chimeric constructs: in one, DNA encoding a Rubisco small subunit-3A plastid transit peptide is fused in-frame to the 5' end of the PheA open reading frame to direct the bacterial enzyme in to the plastid where aromatic amino acid biosynthesis is localized. The second construct lacks the Rubisco small subunit-3A plastid transit peptide in order to test whether aromatic amino acid metabolism is strictly localized to the plastid or whether at least some parts of it operate in the cytosol. Both constructs transformed into Arabidopsis plants, and homozygous T2 plants are generated
-
overexpressed in Escherichia coli
overexpressed in Escherichia coli KA13, transformed with the plasmid pAKZ11
-
pheA gene is cloned and expressed in Escherichia coli
-
the catalytic and regulatory domains of prephenate dehydratase are individually cloned in the NdeI/XhoI sites of pET23a vector and expressed in Escherichia coli BL21(DE3)
-