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4.2.1.49: urocanate hydratase

This is an abbreviated version!
For detailed information about urocanate hydratase, go to the full flat file.

Word Map on EC 4.2.1.49

Reaction

3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate
=
Urocanate
+
H2O

Synonyms

Urocanase, Hydratase, urocanate, 4'-Imidazolone-5'-propionate hydro-lyase, 4-Imidazolone-5'-propionate hydrolyase, Imidazolonepropionate hydrolase, 4,5-dihydro-4-oxo-5-imidazole-propanoate hydro-lyase, UROC1, HutU

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.49 urocanate hydratase

Crystallization

Crystallization on EC 4.2.1.49 - urocanate hydratase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging-drop vapour-diffusion method, crystallized after removing one of the seven free thiol groups. The crystal structure is solved by multiwavelength anomalous diffraction using a seleno-methionine derivative and then refined at 1.14 A resolution
the enzyme is crystallized in several conditions, with the best crystals obtained with 0.04 M dipotassium hydrogen phosphate, 16.00 % (w/v) polyethylene glycol 8000 and 22.0% (v/v) glycerol. X ray diffraction data are collected to 2.61 A resolution. The crystals belong to the monoclinic space group P21, with unit cell parameters a = 85.12, b = 137.63, c = 117.69 A, beta = 94.69° and are suitable for molecular replacement phasing