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4.2.1.47: GDP-mannose 4,6-dehydratase

This is an abbreviated version!
For detailed information about GDP-mannose 4,6-dehydratase, go to the full flat file.

Word Map on EC 4.2.1.47

Reaction

GDP-alpha-D-mannose
=
GDP-4-dehydro-alpha-D-rhamnose
 +
H2O

Synonyms

ATCV-1GMD, bceN, Dm-gmd, GDP-alpha-D-mannose 4,6-dehydratase, GDP-D-mannose 4,6-dehydratase, GDP-D-mannose 4-oxido-6-reductase, GDP-D-mannose dehydratase, GDP-D-mannose-4,6-dehydratase, GDP-mannose 4, 6-dehydratase, GDP-mannose 4,6 dehydratase, GDP-mannose 4,6-dehydratase, GDP-mannose dehydratase, GDP-mannose dehydrogenase, GDP-mannose-4,6-dehydratase, GMD, GMDS, guanosine 5'-diphosphate-D-mannose 4,6-dehydratase, Guanosine 5'-diphosphate-D-mannose oxidoreductase, Guanosine diphosphomannose 4,6-dehydratase, Guanosine diphosphomannose oxidoreductase, L-GMD, M-GMD, MUR1, ORF13.7, PBCV-1 GMD, S-GMD

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.47 GDP-mannose 4,6-dehydratase

Cloned

Cloned on EC 4.2.1.47 - GDP-mannose 4,6-dehydratase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
as fusion protein
-
coexpression with GDP-4-keto-6-deioxymannose-3,5-epimerase-4-reductase, which is required for enzymatic activity after expresseion in Saccharomyces cerevisiae
-
expressed in Corynebacterium glutamicum strain ATCC 13032
-
expressed in Escherichia coli
-
expressed in Escherichia coli as glutathione S-transferase fusion protein
expressed in Escherichia coli BL21(DE3) cells
expression in Agrobacterium tumefaciens
expression in Escherichia coli
GDP-D-mannose-4,6-dehydratase and GDP-4-keto-6-deoxymannose 3, 5-epimerase 4-reductase, the two crucial enzymes for the de novo GDP-L-fucose biosynthesis, are overexpressed in recombinant Escherichia coli by constructing inducible overexpression vectors. Optimum expression conditions in recombinant Escherichia coli BL21(DE3) are 25°C and 0.1 mM isopropyl-beta-D-thioglucopyranoside. Maximum GDP-L-fucose concentration of 38.9 mg/l is obtained in a glucose-limited fed-batch cultivation, and it is enhanced further by coexpression of NADPH-regenerating glucose-6-phosphate dehydrogenase encoded by the zwf gene to achieve 55.2 mg/l GDP-L-fucose under the same cultivation condition
-
GMD DNA and amino acid sequence determination and analysis
GMD sequence determination and analysis, sequence comparison and phylogenetic tree, functional overexpression of soluble GMD in Escherichia coli strain BL21(DE3) from an IPTG-inducible expression vector, optimum expression conditions are 30°C and 0.1 mM IPTG
-
His6-GMD expressed from the pQE-30-gmd vector
into pET28a+ to construct pLW1534, expressed in Escherichia coli BL21 as His-tagged fusion protein
L-GMD, S-GMD and M-GMD, expressed in Escherichia coli
recombinant expression of His-tagged GMD in Escherichia coli