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4.2.1.34: (S)-2-methylmalate dehydratase

This is an abbreviated version!
For detailed information about (S)-2-methylmalate dehydratase, go to the full flat file.

Word Map on EC 4.2.1.34

Reaction

(S)-2-methylmalate
=
2-methylfumarate
+
H2O

Synonyms

(+)-Citramalic hydro-lyase, Bxe_A3136, Dehydratase, citramalate, FumA, fumarase A, fumarase/mesaconase, FumD, Hydratase, mesaconate, Mesaconase, Mesaconase, mesaconate, mesaconase/fumarase, Mesaconate hydratase

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.34 (S)-2-methylmalate dehydratase

Storage Stability

Storage Stability on EC 4.2.1.34 - (S)-2-methylmalate dehydratase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-10°C, pH 8.4, protein concentration above 1 mg/ml in 0.1 M Tris-HCl buffer, component I is stable for months
-
-196°C, activity of component II remains constant for at least 8 months
-
-20°C, in presence of 20% (NH4)2SO4 or 10% glycerol in air, about 80-90% can be reactivated even after several months
Aerobic bacterium
-
4°C, 70% loss of activity after 1 week under N2
Aerobic bacterium
-
storage of the protein for 6 months leads to almost complete loss of its activity, which can be fully restored by the reactivation with Fe2+ and thiol