4.2.1.34: (S)-2-methylmalate dehydratase
This is an abbreviated version!
For detailed information about (S)-2-methylmalate dehydratase, go to the full flat file.
Reaction
Synonyms
(+)-Citramalic hydro-lyase, Bxe_A3136, Dehydratase, citramalate, FumA, fumarase A, fumarase/mesaconase, FumD, Hydratase, mesaconate, Mesaconase, Mesaconase, mesaconate, mesaconase/fumarase, Mesaconate hydratase
ECTree
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Oxidation Stability
Oxidation Stability on EC 4.2.1.34 - (S)-2-methylmalate dehydratase
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4°C, 90% loss of activity after 24 h in air. Reactivation by Fe2+ and 2-mercaptoethanol
Aerobic bacterium
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5667
aerobic purification results in inactive protein, which can only partly be reactivated with Fe2+ and thiol
749072
inactivation by exposure to oxygen, full activation by anaerobic incubation with a sulfhydryl compound for 1-2 hours at 37°C
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5665
inactivation by oxygen. Reactivation by incubating the two protein components with a reducing agent and Fe2+ in absence of oxygen. Mn2+ inhibits activation. Effect of temperature and pH on activation
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5669
the enzyme is oxygen sensitive, and the aerobically measured activity of the (aerobically) purified protein is relatively low. The incubation of the enzyme with Fe2+ and thiol and following measurement of the activity under strictly anaerobic conditions leads to an 4fold increase in fumarate hydratase activity
746828