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4.2.1.34: (S)-2-methylmalate dehydratase

This is an abbreviated version!
For detailed information about (S)-2-methylmalate dehydratase, go to the full flat file.

Word Map on EC 4.2.1.34

Reaction

(S)-2-methylmalate
=
2-methylfumarate
+
H2O

Synonyms

(+)-Citramalic hydro-lyase, Bxe_A3136, Dehydratase, citramalate, FumA, fumarase A, fumarase/mesaconase, FumD, Hydratase, mesaconate, Mesaconase, Mesaconase, mesaconate, mesaconase/fumarase, Mesaconate hydratase

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.34 (S)-2-methylmalate dehydratase

Oxidation Stability

Oxidation Stability on EC 4.2.1.34 - (S)-2-methylmalate dehydratase

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OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, 90% loss of activity after 24 h in air. Reactivation by Fe2+ and 2-mercaptoethanol
Aerobic bacterium
-
5667
aerobic purification results in inactive protein, which can only partly be reactivated with Fe2+ and thiol
749072
inactivation by exposure to oxygen, full activation by anaerobic incubation with a sulfhydryl compound for 1-2 hours at 37°C
-
5665
inactivation by oxygen. Reactivation by incubating the two protein components with a reducing agent and Fe2+ in absence of oxygen. Mn2+ inhibits activation. Effect of temperature and pH on activation
-
5669
the enzyme is oxygen sensitive, and the aerobically measured activity of the (aerobically) purified protein is relatively low. The incubation of the enzyme with Fe2+ and thiol and following measurement of the activity under strictly anaerobic conditions leads to an 4fold increase in fumarate hydratase activity
746828