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4.2.1.10: 3-dehydroquinate dehydratase

This is an abbreviated version!
For detailed information about 3-dehydroquinate dehydratase, go to the full flat file.

Word Map on EC 4.2.1.10

Reaction

3-dehydroquinate
=
3-dehydroshikimate
 +
H2O

Synonyms

3-dehydroquinase, 3-dehydroquinate dehydratase, 3-dehydroquinate dehydratase /shikimate dehydrogenase isoform 1, 3-dehydroquinate dehydratase /shikimate dehydrogenase isoform 2, 3-dehydroquinate dehydratase/shikimate dehydrogenase, 3-DHQase, 5-dehydroquinase, 5-dehydroquinate dehydratase, 5-dehydroquinate hydro-lyase, AfDQ, aroD, AroQ, bifunctional 3-dehydroquinate dehydratase/shikimate dehydrogenase, chloroplast, dehydratase, 3-dehydroquinate, dehydroquinase, dehydroquinate dehydratase, dehydroquinate dehydratase-shikimate dehydrogenase, dehydroquinate synthase, DHD/SHD, DHQ synthase, DHQ-SDH, DHQ-SDH protein, DHQ/SDH, DHQ1, DHQ2, DHQase, DHQase I, DHQase-SORase, DHQD, DHQS, DQD, EC 4.2.1.10, EcDQD/SDH1, EcDQD/SDH2, mDQD, membrane-bound 3-dehydroquinate dehydratase, More, MtDHQase, NtDHD/SHD-1, NtDHD/SHD-2, pDQD, sDQD, SVCR3, type I 3-dehydroquinate dehydratase, Type I dehydroquinase, type I dehydroquinate dehydratase, Type I DHQase, type I DHQD, type II 3-dehydroquinate dehydratase, Type II dehydroquinase, Type II DHQase

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.10 3-dehydroquinate dehydratase

Purification

Purification on EC 4.2.1.10 - 3-dehydroquinate dehydratase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
bifunctional enzyme with activities of ec 1.1.1.25 and ec 4.2.1.10
-
catabolic isoenzyme
-
His-Trap HP Ni Sepharose column chromatography, and S-200 gel filtration
-
HiTrap-Q anion-exchange media is used
multienzyme complex with the activities of ec 1.1.1.25, ec 4.2.1.10, 5-dehydroquinate synthetase, 3-enolpyruvylshikimic acid 5-phosphate synthetase and shikimic acid kinase
-
Ni-NTA column chromatography
periplasmic and cytosolic 3-dehydroquinate dehydratases are purified using a DEAE-cellulose, a DEAE-Sephadex A-50, a Hydroxyapatite and a Sephadex G-200 column
-
purified by immobilized nickel-ion chromatography
-
Q Sepharose column chromatography, hydroxyapatite column chromatography, and Mono Q column chromatography
recombinant His-tagged DHQD from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, cleavage of the His-tag, and gel filtration
SuperQ Toyopearl 650 column chromatography, Resource Q column chromatography, hydroxylapatite column chromatography, and Superdex 200 gel filtration
-
using a Q Sepharose, a phenyl Sepharose, a hydroxyapatite and a Sephacryl S300 column